EVALUATION OF THE EFFECT OF ENDONUCLEASE ON EXTRACELLULAR NEUTROPHILIC TRAPS

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Abstract

The paper presents the results of the analysis of the dynamics of the formation of extracellular DNA networks by neutrophils isolated from peripheral blood and their fragmentation. During the experiment, it was found that deoxyribonuclease I (DNase I) cleaves DNA – the basis of extracellular traps. The ability to secrete this enzyme component in some pathogenic bacteria (S. pyogenes, S. aureus or S. pneumoniae) probably explains their ability to release from extracellular structures.

About the authors

I. V. Samuseva

South Ural Medical State University

Author for correspondence.
Email: i.v.samuseva@yandex.ru

Junior Researcher, Research Institute of Immunology, 

Chelyabinsk

Russian Federation

A. Y. Savochkina

South Ural Medical State University

Email: fake@neicon.ru

MD, Professor of the Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics,

Chelyabinsk

Russian Federation

K. V. Nikushkina

South Ural Medical State University

Email: fake@neicon.ru

PhD, Leading Researcher, Research Institute of Immunology,

Chelyabinsk

Russian Federation

I. V. Yemelyanov

South Ural Medical State University;
Chelyabinsk State University

Email: fake@neicon.ru

Senior Assistant, Research Institute of Immunology,

Chelyabinsk

Russian Federation

T. I. Nikonova

South Ural Medical State University

Email: fake@neicon.ru

Senior Assistant, Research Institute of Immunology,

Chelyabinsk

Russian Federation

M. A. Zotova

South Ural Medical State University

Email: fake@neicon.ru

PhD, Researcher, Research Institute of Immunology, 

Chelyabinsk

Russian Federation

References

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  2. Herant E. Mechanisms of neutrophil phagocytosis. Journal of cell science 2003,19; 45–50
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  4. Halliwell B, Gutteridge J., Modulating A. Role for Antioxidants in Desiccation Tolerance/ Halliwell B. Biology 1998, l (45); 734–740
  5. Гольдштейн Н. И. Активные формы кислорода как жизненно необходимые компоненты воздушной среды. Биохимия, 2002, 67; 194–204.
  6. Delanty N., Dichter M. Oxidative injury in the nervous system. Acta Neurologica Scandinavica 1998, 9; 145–153.
  7. Савочкина А. Ю. Нейтрофильные внеклеточные ловушки: механизмы образования, методы обнаружения, биологическая роль: диссертация доктора медицинских наук. Челябинская государственная медицинская академия. – Челябинск, 2012; 212.

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Copyright (c) 2019 Samuseva I.V., Savochkina A.Y., Nikushkina K.V., Yemelyanov I.V., Nikonova T.I., Zotova M.A.

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