GLYCOARRAYS FOR STUDYING OF GLYCAN-PROTEIN INTERACTION

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Abstract

The capacities of glycoarray – a unique method for investigation of carbohydrate-binding proteins including antibodies and lectins – are presented. It was shown that array is very flexi ble device and it can be directional changed for solution of a wide range of diff erent tasks of both fundamental and applied nature.

About the authors

N. V. Shilova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov of the Ministry of Healthcare of Russian Federation;
Federal Budget Institution of Science «M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry»;
Semiotik LLC

Author for correspondence.
Email: pumatnv@gmail.com

PhD, senior scientist of Department of chemical biology of glycans and lipids;

senior scientist of laboratory of clinical immunology;

chief microchip specialist,

Moscow

Russian Federation

N. R. Khasbiullina

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov of the Ministry of Healthcare of Russian Federation;
Semiotik LLC;
Federal Budget Institution of Science «N. D. Zelinsky Institute of Organic Chemistry»

Email: fake@neicon.ru

junior scientist of laboratory of carbohydrate chemistry;

staff scientist of laboratory of clinical immunology;

microchip specialist of Semiotik LLC,

Moscow

Russian Federation

A. Yu. Nokel

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov of the Ministry of Healthcare of Russian Federation;
Semiotik LLC

Email: fake@neicon.ru

PhD, senior scientist of laboratory of clinical immunology;

chief engineer,

Moscow

Russian Federation

M. M. Ziganshina

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov of the Ministry of Healthcare of Russian Federation

Email: fake@neicon.ru

PhD, senior scientist of laboratory of clinical immunology,

Moscow

Russian Federation

N. V. Bovin

Federal Budget Institution of Science «M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry»

Email: fake@neicon.ru

Dr. habil., professor, chief of Department of chemical biology of glycans and lipids,

Moscow

Russian Federation

References

  1. Бовин Н. В. Естественные антитела к гликанам. Биохимия. 2013, 78(7), 1008.
  2. Зиганшина М. М., Шилова Н. В., Хасбиуллина Н. Р., Ракитько А. С., Тютюнник Н. В., Мантрова Д. А., Тютюнник В. Л., Бовин Н. В., Сухих Г. Т. Спектр и диагностическое значение антигликановых антител, выявляемых при развитии больших акушерских синдромов, связанных с дефектами плацентации. Росс. Аллергол. Журн. 2018, 15(1), 37.
  3. Butvilovskaya V. I., Popletaeva S. B., Chechetkin V. R., Zubtsova Z. I., Tsybulskaya M. V., Samokhina L. O., Vinnitskii L. I., Ragimov A. A., Pozharitskaya E. I., Grigor Eva G. A., Meshalkina N. Y., Golysheva S. V., Shilova N. V., Bovin N. V., Zasedatelev A. S., Rubina A. Y. Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips. Cancer Med. 2016. 5(7), 1361.
  4. Ludwig A.K., Michalak M., Shilova N., André S., Kaltner H., Bovin N. V., Kopitz J., Gabius H. J. Studying the Structural Significance of Galectin Design by Playing a Modular Puzzle: Homodimer Generation from Human Tandem-Repeat-Type (Heterodimeric) Galectin-8 by Domain Shuffling. Molecules. 2017, 22(9), 1572.

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Copyright (c) 2019 Shilova N.V., Khasbiullina N.R., Nokel A.Y., Ziganshina M.M., Bovin N.V.

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