EFFECT OF PREGNANCY-SPECIFIC β1-GLYCOPROTEIN ON THE DIFFERENTIATION AND PROLIFERATION OF TH17-POLARIZED HELPER T CELLS

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Abstract

The effect of the native (non-recombinant) pregnancy specific β1-glycoprotein (PSG) preparation on the differentiation and proliferation of Th-17 polarized helper T cells was studied. The object of the study was the culture of “naive” helper T cells (CD4+) isolated by the immunomagnetic separation. Cells were polarized into the Th17 phenotype using TCR-activator and pro-inflammatory cytokines (IL-1β and IL-6). It was shown that PSG had a suppressive effect on the Th17 cell frequency in culture (CD4+ROR-γt+), and inhibited the proliferation of these cells, as measured by the method of differential gating. Thus, in the experimental model used, PSG had a pronounced suppressive effect on the differentiation and proliferation of T-helpers polarized into the Th17 phenotype.

About the authors

V. P. Timganova

«Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences»

Author for correspondence.
Email: timganovavp@gmail.com

Ph.D. (Biology), junior researcher, laboratory of ecological immunology,

Perm

Russian Federation

M. S. Bochkova

«Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences»

Email: fake@neicon.ru

Ph.D. (Biology), researcher, laboratory of ecological immunology,

Perm

Russian Federation

A. P. Kalugina

Perm State University

Email: fake@neicon.ru

student of the department of microbiology and immunology,

Perm

Russian Federation

P. V. Khramtsov

«Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences»;
Perm State University

Email: fake@neicon.ru

Ph.D. (Biology), researcher, laboratory of ecological immunology;

assistant professor of the department of microbiology and immunology, Faculty of Biology,

Perm

Russian Federation

M. B. Raev

«Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences»;
Perm State University

Email: fake@neicon.ru

Ph.D., MD (Biology), Leading Researcher, Laboratory of Ecological Immunology;

professor of the department of microbiology and immunology, Faculty of Biology,

Perm

Russian Federation

S. A. Zamorina

«Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences»;
Perm State University

Email: fake@neicon.ru

Ph.D., MD (Biology), leading researcher, laboratory of ecological immunology;

professor of the department of microbiology and immunology, Faculty of Biology,

Perm

Russian Federation

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Copyright (c) 2019 Timganova V.P., Bochkova M.S., Kalugina A.P., Khramtsov P.V., Raev M.B., Zamorina S.A.

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