IDIOTYPICAL MECHANISMS OF INDUCTION AND REGULATION OF AUTOIMMUNE REACTIONS IN EXPERIMENTAL AUTOIMUNE ENTSEPHALOMYELITIS IN RATS

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Abstract

The aim of research was to confirm that induction and regulation of autoreactive lymphocytes in model of the experimental autoimmune encephalomyelitis (EAE) in rats is carried out indirectly through idiotip-antiidiotipic (IAI) interactions with participation of the lymphocytes producing antiidiotipic antibodies with rheumatoid factors (RF) properties. Methods. Rats were immunized by the main guinea pig myelin protein (MMPgp). The kinetics of antibodies and autoantibodies formation during the immune response was investigated. MMPgp antibodies and MMP rats autoantibodies were determined by ELISA. RF was determined during agglutination test of tannised loaded IgG erythrocytes. The presence of IAI interactions was studied by competitive ELISA method. Results. Existence of IAI interactions between MMPgp antibodies and RF on the one hand, and, between MMP rats antibodies and RF on another hand was demonstrated. Association between RF high level in a blood and lack of clinical signs of EAE was found in immunized animals. Conclusion. The mechanism of induction of MMP rats autoantibodies during MMPgp immunization is mediated by IAI interactions of that clones of lymphocytes, which producing antiidiotipic antibodies with RF properties. Antiidiotipic antibodies with RF properties is carry out a specific negative regulation of a humoral immune response against a heterological antigen inductor of EAE, MMPgp through IAI interactions.

About the authors

P. V. Ivanov

Federal State Budgetary Educational Institution of Higher Education «Udmurt State University»

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

I. V. Menshikov

Federal State Budgetary Educational Institution of Higher Education «Udmurt State University»

Email: noemail@neicon.ru
Russian Federation

L. V. Beduleva

Federal State Budgetary Educational Institution of Higher Education «Udmurt State University»

Email: noemail@neicon.ru
Russian Federation

References

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  2. Stahl D. et al. Analysis of human self-reactive antibody repertoires by quantitative immunoblotting. J Immunol Methods. 2000 Jun 23;240(1-2):1-14.
  3. Beduleva L., Menshikov I. Role of idiotype-anti-idiotype interactions in the induction of collagen-induced arthritis in rats. Immunobiology. 2010 Dec;215(12):963-70
  4. Menshikov I., Beduleva L. Evidence in favor of a role of idiotypic network in autoimmune hemolyt-ic anemia induction: theoretical and experimental studies. Int Immunol. 2008 Feb;20(2):193-8.

Copyright (c) 2017 Ivanov P.V., Menshikov I.V., Beduleva L.V.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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