INVESTIGATION OF GROWTH AND VIABILITY OF HYBRIDOMA BAP3 CELLS IN DIFFERENT CULTURE MEDIA IN VITRO

Cover Page

Cite item

Full Text

Abstract

Growth and viability in different culture media after thawing of hybridoma BAP3 cells that produces monoclonal antibodies against pregnancy-specific b1-glycoprotein (PSG) were studied in vitro. We show that cultivation in [DMEM+12 % FCS] leads to maximal cell growth (1,8-fold in two days) with retention of viability of more than 70 %. There were 1,1-1,2-fold growth and viability of 55-59 % in [DCCM-1]-based media and 1,6-fold growth in two days in [RPMI-1640+12 % FCS]. Therefore, application of [DMEM+12 % FCS] medium seems to be the most appropriate for cultivation of hybridoma BAP3 cells after their thawing from cryopreservation.

About the authors

M. B. Raev

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

S. A. Zamorina

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

V. P. Timganova

Institute of Ecology and Genetics of Microorganisms UB RAS

Email: noemail@neicon.ru
Russian Federation

M. S. Bochkova

Institute of Ecology and Genetics of Microorganisms UB RAS

Email: noemail@neicon.ru
Russian Federation

P. V. Khramtsov

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

References

  1. Раев М. Б. Способ выделения и очистки трофо-бластического β-1-гликопротеина. Патент РФ № 2367449, опубликован 20.09.2009, Бюл. № 26.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2017 Raev M.B., Zamorina S.A., Timganova V.P., Bochkova M.S., Khramtsov P.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies