Vol 22, No 3 (2019)

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Full Issue

ORIGINAL ARTICLES

COMPARATIVE EVALUATION OF INDICATORS OF APOPTOSIS AND PROLIFERATION IN THE ISOLATED FRACTURE OF THE FEMORAL BONE IN CONDITIONS OF SYSTEM OZONOTHERAPY

Abramov K.S., Davydova E.V., Osikov M.V.

Abstract

The cytometric parameters of apoptosis and proliferation were studied in 20 men with an isolated fracture of the femur under the conditions of using systemic ozone therapy. It is shown that in case of a fracture of the femur, there is an increase in the absolute and relative number of T-lymphocytes with the expression of receptor readiness for apoptosis and the level of lymphocytes with cytometric signs of apoptosis (hypodiploid peak). The use of ozone therapy has a positive effect on the mechanisms of survival of immune cells, regardless of their phenotype, by increasing the expression of bcl-2 to the values of the control group, which can be regarded as one of the mechanisms that allow to regulate and carry out the selection of effective B-lymphocytes capable of accumulating a sufficient anti-infective antibodies in response to injury.
Russian Journal of Immunology. 2019;22(3):1085-1088
pages 1085-1088 views

ASSESSMENT OF CELL-MEDIATED IMMUNITY IN WORKERS EMPLOYED IN UNDERGROUND CHROME ORE MINING

Alikina I.N.

Abstract

Immunological study of the violation of cellular component of immune system in workers employed in chrome ore mining revealed excessive production of suppressor T-regulatory cells CD4+CD127, apoptotic Bax protein. What is more, there was an increase in the rate of specific sensitization of IgE to chrome in relation to the comparison group associated with elevated chrome level in the blood (more than 5 times in relation to the reference level). A significant decrease in CD-immunogram parameters was found: CD3+CD25+, CD3+CD95+. Besides, there was reduction in expression of intracellular protein factor Bcl-2, transcription factor regulating the cell cycle – p53, receptor for tumor necrosis factor TNFR compared with the values of employees working outside the influence of the studied industrial factors (p<0.05). Thus, the production conditions associated with hapten load (chrome) create an imbalance of cellular regulation, disrupting the programmed procedure of cell death (apoptosis). Key words: clusters of cell differentiation, specific sensitization, chrome>˂ 0.05). Thus, the production conditions associated with hapten load (chrome) create an imbalance of cellular regulation, disrupting the programmed procedure of cell death (apoptosis). 

 

Russian Journal of Immunology. 2019;22(3):1089-1092
pages 1089-1092 views

IMMUNOLOGICAL EVALUATION AND TREATMENT OF RESISTANCE ACUTE MYELOID LEUKEMIA IN YOUNG ADULT (CASE REPORT)

Vinogradov A.V., Rezaykin A.V., Partylova E.A., Sazonov S.V., Sergeev A.G.

Abstract

Aim: to analyze the case of immunological detection and treatment of young male with resistant acute myeloid leukemia in Sverdlovsk Regional Hematological Centre. Bone marrow and peripheral blood samples obtained from male, aged 38, treated using intensive chemotherapy programs and then obtained supportive care and palliative chemotherapy in municipal hospital. Immunohistochemical characterization of AML blast cells: medium and small cells with one or several nucleoli, MPO, CD34, CD117, CD68 positive. In cytochemistry lipids were positive in 29.0% of blasts, glycogen – 81.0%. Immunophenotype of blast cells was CD45, CD13, CD33, CD34, CD38, CD117, MPO-cyt (+). Karyotype was 47, XY, +13 and changed during AML clonal progression. In case, two non-synonymous substitution were co-existed in samples: NRAS gene с. 182 A>C and TP53 gene с. 215 С>G. The number of NRAS gene point mutations changed during AML clonal progression (additional c. 35 G>С substitution was found). Immunophenotype of blast cells not changed during progression. Overall time of patient observation was 42 week.
Russian Journal of Immunology. 2019;22(3):1093-1098
pages 1093-1098 views

THE CONTENTS OF CYTOKINES IN SEMEN IN INFERTILITY ASSOCIATED WITH CHRONIC PROSTATITIS

Galimova E.F., Travnikov O.Y., Khaibullina Z.G., Murtazina A.Z., Asadullina T.S., Galimov S.N.

Abstract

The study assessed using the ELISA method the content of 16 cytokines in seminal plasma of 85 patients with chronic prostatitis and concomitant infertility in order to determine their significance as potential markers of prognosis and treatment efficacy. A statistically significant increase in the concentration of IFN-γ and IL-18 was established against the background of a significant decrease in the levels of IL-1β, TGF-β, SDF-1α and IL-7. The most informative predictor of the limitation of the fertilizing ability of spermatozoa in inflammation of the prostate gland is the imbalance of IL-18 and TGF-β as key regulators of the immune status of the reproductive system.
Russian Journal of Immunology. 2019;22(3):1099-1102
pages 1099-1102 views

THE INFLUENCE OF A NUMBER OF SUBSTITUTED 1,3,4-6H-THIADIAZINES ON THE CONTENT OF CYTOKINES IN BLOOD PLASMA OF RATS WITH EXPERIMENTAL DIABETES MELLITUS TYPE2

Gette I.F., Emelianov V.V., Belousova A.V., Danilova I.G., Sidorova L.P., Tseitler T.A.

Abstract

A study was carried out to investigate the impact of the L-17, a representative of 1,3,4-6H-thiadiazines, on the cytokine spectrum of rat blood plasma when simulating type 2 diabetes mellitus. Doses of nicotinamide and streptozotocin were respectively, 110 mg / kg and 65 mg / kg. The introduction of L-17 to diabetic rats was accompanied by a decrease in hyperglycemia and a partial correction of the imbalance in the content of pro-inflammatory and anti-inflammatory cytokines: a decrease in the level of IL-1α and IFN-γ without normalization of the IL-10 and TGF-β1 level.
Russian Journal of Immunology. 2019;22(3):1103-1107
pages 1103-1107 views

BACTERICIDAL EFFECTS OF MONOCHROMATIC LIGHT 635 NM AND 450 NM, WITH RESPECT TO STAPHYLOCOCCUS AUREUS IN PATIENTS WITH STAFILODERMY

Gizinger O.A., Ziganshin O.R., Laknitskaya A.O.

Abstract

The paper presents the results of a study of the antibacterial properties of monochromatic incoherent light with wavelengths of 635 nm with an average power of 50 mW, a density of 5 J / cm2 and 450 nm with an average power of 50 mW, an energy density of 5 J / cm2 , the area of the illuminated zone – 9.25 cm2 , illumination – 11 mW / cm2 in vitro using Staphylococcus aureus strains isolated from the skin of patients with staphylococcal skin lesions, the exposure time to the isolates of microorganisms is 900 seconds, the area of the illuminated zone is 9.25 cm2 , the illumination is 11 mW / cm2 . It was established that light with a wavelength of 635 ± 10 nm has poorly pronounced bacteriostatic effects on Staphylococcus aureus, which was confirmed by the growth of Staphylococcus aureus in isolates after irradiation, light with a wavelength of 450 ± 10 nm, has bacteriostatic properties, which was confirmed by a decrease in the number of Staphylococcus aureus colonies after exposure to light with a wavelength of 450 ± 10 nm. 

Russian Journal of Immunology. 2019;22(3):1108-1112
pages 1108-1112 views

EFFICIENCY OF COMPLEX THERAPY OF CHRONIC ADENOIDITIS USING MONOCHROMATIC COHERENT RADIATION WITH A WAVE LENGTH OF 635 NM

Gizinger O.A., Kornova N.V., Zyryanova K.S., Ivanova R.A., Nazarova V.E.

Abstract

The paper presents the results of a study of the effectiveness of complex therapy of chronic adenoiditis using monochromatic coherent radiation with a wavelength of 635 nm generated by the Mustang apparatus. Radiation parameters: 635 nm wavelength, output power 6–8 mW, endonasal, exposure time 60 s, dose radiation of 0.5 J / cm2 , a course of 10 sessions. It was established that the use of monochromatic coherent radiation with a wavelength of 635 nm contributed to the normalization of factors of innate and adaptive peripheral immunity Blood Coy, landscape restoration lymphocyte receptor, reduced the degree of contamination of pathogenic microorganisms pharyngeal tonsils, reduced the number of exacerbations of chronic adenoiditis. 

Russian Journal of Immunology. 2019;22(3):1113-1117
pages 1113-1117 views

IMMUNOPOIESIS-MODULATING PROPERTIES OF BIOFLAVONOIDES ORIGINAL COMPLEX «EPIGENORM ANTIVIR»

Goldina I.A., Markova E.V., Orlovskaya I.A., Toporkova L.B.

Abstract

Food plants bioflavonoids have chemoprotective and immunomodulatory properties, including the ability to affect the earliest stages of immunopoiesis, therefore the development of complex adjuvant therapeutic compositions on their basis is of undoubted relevance. The purpose of this study was to identify the immunopoiesis modulating and chemoprotective properties of the natural food polyphenols original complex “Epigenorm antivir”. As a result of the study, it was found that “Epigenorm antivir” increases the functional activity of bone marrow stem cells – the early common precursors of hematopoiesis, and also it has chemoprotective properties, the ability to increasing of the hematopoietic stem cell functional activity under the immunosuppression conditions.
Russian Journal of Immunology. 2019;22(3):1118-1124
pages 1118-1124 views

ASSESSMENT OF CD127⁻ AND CD95⁺ EXPRESSION AND GENETIC PROFILE OF WOMEN OF REPRODUCTIVE AGE EXPOSED TO PHENOL

Dolgikh O.V., Kazakova O.A., Krivtsov A.V.

Abstract

The paper presents the results of immunological and genetic blood studies of women of reproductive age living permanently under aerogenic exposure to phenol and having reproductive disorders as recurrent pregnancy loss. Immunological values – expression of T-regulatory CD127 lymphocytes and expression of CD95⁺ lymphocytes involved in apoptosis were studied. The genetic profile of women was analyzed and the polymorphic variants of genes FOXP3 T3499C rs3761547 and FAS C14405T rs1159120 participating in the expression of clusters were determined. It was found out that women having reproductive disorders and living under increased contamination of biological media with phenol have reduced level of expression of T-regulatory cells CD127 relative to healthy and unexposed women, and a lower level of CD95⁺ cells relative to the unexposed women and the norms. It results in malignant course of processes of reproductive disorders (recurrent pregnancy loss). 

Russian Journal of Immunology. 2019;22(3):1125-1128
pages 1125-1128 views

IMMUNOBIOCHEMICAL INDICATORS AND COMPARATIVE EVALUATION OF POLYMORPHISM OF CHILDREN’S POPULATION GENES IN SOUTH SIBERIA

Dolgikh O.V., Mazunina A.A., Krivtsov A.V., Guselnikov M.A.

Abstract

The analysis of the features of immunoregulatory indicators of the child population living in the zone of airborne aluminum pollution was carried out. According to the results of immunological studies of the health of the child population, the presence of pathological changes in the immune system was revealed, which are manifested in a decrease in the phagocytic activity of cells, an increase in the level of the main classes of immunoglobulins, activation of T cell receptors CD127, an increase in the content of cell death receptor CD95+ (р<0,05). The comparative polymorphism of the eNOS gene (rs1799983) was studied. The excessive frequency of the T allele of the eNOS gene (rs1799983) was established, which, under the conditions of the technogenic “aluminum” province, can cause the occurrence of diseases of the cardiovascular system in children. Key words: aluminum, children population, eNOS gene polymorphism, risk, diseases of the cardiovascular system>˂0,05). The comparative polymorphism of the eNOS gene (rs1799983) was studied. The excessive frequency of the T allele of the eNOS gene (rs1799983) was established, which, under the conditions of the technogenic “aluminum” province, can cause the occurrence of diseases of the cardiovascular system in children. 

Russian Journal of Immunology. 2019;22(3):1129-1133
pages 1129-1133 views

COMPARATIVE CHARACTERISTICS OF THE EFFECT OF SUBSTITUTED 1,3,4-6H-THIADIAZINES ON THE PERIPHERAL BLOOD INDICED OF RATS WITH ALLOXAN DIABETES MELLITUS

Emelianov V.V., Danilova I.G., Brilliant S.A., Gette I.F., Sidorova L.P., Tseitler T.A., Klyuyeva Y.N.

Abstract

The article presents data on the effect of substituted 1,3,4-thiadiazines (L-14, L-17) on the change in hematological parameters of rats with alloxan diabetes. The particular interest is the study of hematological parameters of rats in the development of alloxan diabetes mellitus and the possibility of its correction with compounds from a number of substituted 1,3,4-thiadiazines.
Russian Journal of Immunology. 2019;22(3):1134-1139
pages 1134-1139 views

INFLUENCE OF CERULOPLASMIN ON CYTOKINE PRODUCTION DURING CHRONIC PHYSICAL LOADS OF SUBMAXIMAL POWER

Ermolaeva E.N., Sashenkov S.L., Kantyukov S.A., Petuhova V.I., Jakovleva V.P.

Abstract

Exercise was modeled in an experiment on 42 white mongrel rats. Chronic physical exercise submaximal power was modeled by daily swimming for 30 minutes – 21 days: the fi rst seven days the animals swam without load for 30 minutes, the next two weeks with a load of 2% of body weight. On the 9th, 15th and 21st day of the experiment, the animals were additionally subjected to maximum exercise: they swam for 4 minutes with a weight of 20% of their body weight. Ceruloplasmin was administered on the 1st, 4th and 7th day of physical activity, in a total dose of 60 mg / kg body weight. Blood sampling was carried out on the 9th, 15th and 21st day of the experiment. Under the experimental conditions in rats, it was shown that chronic physical exertion of submaximal power leads to the activation of the functional activity of leukocytes and an increase in the production of IL-6 by 15, 21 days and IL-4 by 21 days of the experiment. The introduction of ceruloplasmin during physical exertion of submaximal power levels out elevated levels of cytokines (IL-6, IL-4) and leads them to control indicators. 

Russian Journal of Immunology. 2019;22(3):1140-1144
pages 1140-1144 views

IMMUNOBIOLOGICAL ASPECTS OF THE APPLICATION OF THE NEW METABIOTICS

Zabokritskiy N.A.

Abstract

The paper presents the results of preclinical studies on the choice of microorganisms for the design of a complex probiotic drug with a pronounced hepatoprotective effect. It has been experimentally established that probiotic strains Bacillus subtilis B-2335, B-4759, B-9906 showed the greatest antagonistic activity against pathogenic and opportunistic microorganisms. Experimental data have been obtained that strains Bacillus subtilis B-9906, Bacillus subtilis B-4759 had the ability to produce proteolytic and amylolytic enzymes not only relatively in higher concentrations, but also for earlier periods of cultivation;. It was found that the most promising strains for the design of a combined biohepatoprotector are three strains: Bacillus subtilis B-2335, B-4759, B-9906.
Russian Journal of Immunology. 2019;22(3):1145-1149
pages 1145-1149 views

IMMUNOBIOLOGICAL ASPECTS OF THE APPLICATION OF THE NEW METABIOTICS

Zabokritskiy N.A.

Abstract

The paper presents the results of clinical and laboratory studies of a new metabiotic drug based on biologically active substances of the probiotic strain Bacillus subtilis B-3679. The assessment of the state and dynamics of changes in such integral immunological parameters characterizing the state of health of patients as the level of lysozyme activity and the concentration of s Ig A, and Ig A, M, G in salivary fluid of patients with chronic generalized periodontitis of moderate severity is given. It was found that the metabiotic was safe for use and had a pronounced positive clinical and therapeutic effect, normalizes the microbiocenosis of the oral cavity, which is accompanied by a decrease in the number of opportunistic microflora and an increase in the concentration of representatives of normal microflora. It was experimentally established that the studied metabiotic has a high antagonistic activity against various types of opportunistic microorganisms secreted in patients with periodontitis and promotes increased activity of salivary fluid lysozyme and immunoglobulins A and G.
Russian Journal of Immunology. 2019;22(3):1150-1156
pages 1150-1156 views

INFLUENCE OF ALPHA-FETOPROTEIN ON CYTOKIN PROFILE OF ACTIVATED T-HELPERS

Zamorina S.A., Litvinova L.S., Todosenko N.M., Timganova V.P., Bochkova M.S., Shardina K.Y., Khramtsov P.V., Raev M.B., Chereshnev V.A.

Abstract

The effect of alpha-fetoprotein (AFP) on the pro-infl ammatory cytokine profi le of activated T-helpers, polarazed in phenotyp of Th17. It was found that AFP did not affect the level of IL-4, IL-5, IL-7, IL-8, IL-10, IL-17, MCP-1, MIP-1α, TNF-α, but in high concentrations (50, 100 IU/ml) increased IL-2 production by activated T-helper cells. At the same time, AFP in high concentration (100 IU/ml) suppressed the synthesis of IFN-γ, and in low (10 IU /ml) synthesis of G-CSF and GM-CSF. Thus, new aspects of the AFP action regarding the regulation of the cytokine network of Th17 are demonstrated.
Russian Journal of Immunology. 2019;22(3):1157-1162
pages 1157-1162 views

ESTIMATION OF CYTOKIN-LIKE ACTIVITY OF STAPHYLOCOCCUS AUREUS DEPENDING ON THE AVAILABILITY OF GENETIC DETERMINANT OF STYPHYLOCOCCAL PROTEIN A

Zurochka A.V., Zurochka V.A., Fomina L.O., Fayzullina A.I., Dobrynina M.A., Gritsenko V.A.

Abstract

In this work, the production of cytokine-like substances was studied in 5 strains of Staphylococcus aureus, with and without genetic determinants of staphylococcal protein A (spa gene). The spa gene in S. aureus was determined by the polymerase chain reaction (PCR) method. The cytokine-like activity of the supernatants of S. aureus daily broth cultures was determined using Multiplex (Luminex, United States) immunofluorescence test systems (G-CSF, GM-CSF, INF-γ, IL-1Ra, IL-1β, IL-2, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17a, TGFα, MCP1, MIP1b, TNF-α) on a MAGPIX-100 instrument (USA). It was established that, regardless of the presence or absence of genetic markers of staphylococcal protein A, in the supernatants of the studied strains of S. aureus, cytokine-like substances were detected. Thus, the cytokine-like activity of S. aureus does not depend on the presence in bacteria of the spa gene, which is responsible for the production of staphylococcal protein A.
Russian Journal of Immunology. 2019;22(3):1163-1167
pages 1163-1167 views

DETECTION OF CYTOKINE-LIKE SUBSTANCES WITH THE HELP OF A TEST SYSTEM FOR THE DETERMINATION OF CYTOKINES IN A MOUSE IN STAPHYLOCOCCUS AUREUS, OPPOSITE FOR SPA GENE

Zurochka A.V., Zurochka V.A., Fomina L.O., Fayzullina A.I., Dobrynina M.A., Gritsenko V.A.

Abstract

In the work, cytokine-like activity was studied in 6 strains of Staphylococcus aureus, with and without genetic determinants of staphylococcal protein A (spa gene). The spa gene in S. aureus was determined by the polymerase chain reaction (PCR) method. The production of cytokine-like substances in the supernatants of S. aureus daily broth cultures was determined using an 8-plex immunofl uorescence test system for the determination of mouse cytokines from BIO-RAD (USA) (GM-CSF, INF-γ, IL-1b, IL-2, IL-4, IL-5, IL-10, TNF-α) on the device MAGPIX-100 (USA). It was established that, regardless of the presence or absence of genetic markers of staphylococcal protein A, cytokine-like substances were detected in the supernatants of the studied strains of S. aureus. Thus, the cytokine-like activity of S. aureus, detected using a multiplex mouse test cytokine test system, does not depend on the presence of the spa gene in bacteria, which is responsible for the production of staphylococcal protein A.
Russian Journal of Immunology. 2019;22(3):1168-1172
pages 1168-1172 views

MONOCLONAL ANTIBODY TO HUMAN PD-1 END DETERMINATION OF ITS SPECIFIC ACTIVITY BY FLOW CYTOMETRY

Isaeva E.N., Sineva S.A., Pigareva N.V., Simbirtsev A.S.

Abstract

The cell receptor PD-1 is a member of the CD28. This coinhibitory receptor is a member of subfamily “immunological checkpoints”. For this reason, testing the activities of such preparations is important for clinical medicine. In this study, a human monoclonal anti PD-1 antibody was developed and tested for functional activity by flow cytometry immunophenotyping analysis. This method was demonstrated to be eff ective for protein identifi cation and for fast screening of monoclonal antibody functional activity.
Russian Journal of Immunology. 2019;22(3):1173-1176
pages 1173-1176 views

COMPARISON OF THE EARLY IMMUNE RESPONSE OF MICE IMMUNIZED BY TULAREMIA VACCINE STRAIN AND ITS DERIVATIVES

Kartseva A.S., Silkina M.V., Kalmantaeva O.V., Mironova R.I., Firstova V.V., Pavlov V.M., Shemyakin I.G.

Abstract

The paper presents comparative data of a specific mice immune response to immunization with F. tularensis 15 NIIEG and its derivatives: F. tularensis SRI-1 or F. tularensis 15/23–1ΔrecA. The results of our experiments showed that after immunization of mice with a vaccine strain and its derivatives, antibodies to F. tularensis LPS appeared in the blood of animals and their level did not significantly diff er between groups. Protection levels of these groups of immune mice against challenge with the highly virulent strain of F. tularensis Schu were different. A comparative analysis showed different expression levels of activation markers and co-stimulating molecules were observed on the surface of B and T lymphocytes on day 3 after mice immunization with different strains.
Russian Journal of Immunology. 2019;22(3):1177-1183
pages 1177-1183 views

IMBALANCE IN PERIPHERAL BLOOD “POLARIZED” T-HELPER SUBSETS DURING MULTIPLY SCLEROSIS

Kudryavtsev I.V., Ilves A.G., Il’chenko A.V., Krobinets I.I., Novoselova O.M., Rubanik K.S., Serebryakova M.K., Prakhova L.N.

Abstract

Multiple sclerosis (MS) is the inflammatory disease of the central nervous system characterized by multifocal areas of demyelization and immune cell infiltration. In the current study, we analysed the frequencies and the phenotypes of “polarized” Th cell subsets in peripheral blood of multiple sclerosis patients (n=38, with low disease duration – 1 year (8 month; 1,5 years) and low EDSS – 1,0 (1,0; 1,5)) and healthy control group (n=48). We show that within CD45RACD3+CD4+ subset the frequencies of Th17 and follicular Th (Tfh) cells were signifi cantly higher in patients while the levels of Th1 cells were significantly lower in comparison to control group. Similarly, in central memory CD45RACD62L+ Th the relative number of Th1 cells was reduced while frequencies of Tfh subset were increased in MS patients if compared to control. Finally, MS patients showed increased frequency of Th17 and Tfh cells in CD45RACD62L Th subset compared to control while Th1 were lower in patients group. It seems that Th17 cells infiltrating the nervous tissue could be responsible for neutrophil infiltration, while Tfh cells could take part in B-cell-mediated immune response nearby to site of infl ammation and may be accompanied by production of self-reactive antibodies as well as augmented neutrophilic activity. 

Russian Journal of Immunology. 2019;22(3):1184-1191
pages 1184-1191 views

THE CONTENT OF CD-LYMPHOCYTES AND HUMORAL FACTORS IN ATHLETES WITH A DIFFERENT STATE OF PERIPHERAL BLOOD NEUTROPHILS

Kolupaev V.A., Sashenkov S.L., Mel’nikov I.Y., Lifanov N.A., Kolupaeva I.L.

Abstract

The research is devoted to different rates of CD-lymphocytes and humoral factors in athletes. Athletes were divided into groups according to phagocytic, lysosomal and NBT-activity of neutrophils in peripheral blood. Also we took into account type of muscle energy supply (aerobic or anaerobic) and physical activity dynamics. It was determined that type of physical load has no significant effect on the studied parameters. At the same time, different combinations of the phagocytic and lysosomal activity rates accompanied by differences in CD20+-lymphocytes rate with normal level of NBT-test. At the low level of NBT-activity of neutrophils differences in CD20+-lymphocytes and IgG rates were noted. At the high level of NBT-activity of neutrophils significant differences in CD4, CD20, CD34 - lymphocytes and IgA rates were determined. 

Russian Journal of Immunology. 2019;22(3):1192-1200
pages 1192-1200 views

THE ROLE OF CYTOKINES IN A SURGICAL STRESS RESPONSE TO EARLY POSTOPERATIVE PERIOD IN RHINOSURGERY PATIENTS

Korkmazov A.M., Kornova N.V., Beloshangin A.S., Soltys A.M., Sanyukovich A.A.

Abstract

Тhe paper presents the results of a study of the content of cytokines in washes from the mucous membrane of the nasal cavity in the early postoperative period in patients operated on for the curvature of the nasal septum. It has been established that in rhinosurgical patients on the first day after surgery, an imbalance in the cytokine system is recorded, consisting in increasing the amount of IL-8, TNF-α, IL-1β, IL-4, and the content of IL-2 decreases. IL-6. We regard the results obtained as the result of the compensatory-adaptive response of the nasal mucosa to surgical stress that occurs in the early postoperative period.
Russian Journal of Immunology. 2019;22(3):1201-1205
pages 1201-1205 views

DEPENDABLE EFFECT OF LYOPHYLIZED EXTRACT OF CHICKEN EMBRYO CELLS ON INDUCED APOPTOSIS OF MONOCYTES UNDER IN VITRO CONDITIONS

Kostolomova E.G., Suhovej U.G., Unger I.G.

Abstract

One of the promising areas is the use of new technologies, in particular the development of drugs that have the ability to neutralize and eliminate pathogenic microorganisms that penetrate well into the wound and have reparative properties. The research results showed that the embryonic tissues of chickens have signifi cant prospects for further use as a substrate in the preparation of various forms of effective biologics. A more in-depth study of the mechanisms of reparative regeneration of skin wounds, in particular the role of apoptosis, can facilitate the search for new approaches to therapy associated with effects on immune mechanisms regulating cell death and assessing the effectiveness of the drugs used.
Russian Journal of Immunology. 2019;22(3):1206-1210
pages 1206-1210 views

IMMUNOLOGICAL FEATURES OF THE DEVELOPMENT EARLY FORM PERIIMPLANTS AND THE TOPICAL IMMUNOTHERAPY EFFECT ON THE SALIVATOR LEVEL CYTOKINES DURING DENTAL IMPLANTATION

Latyushina L.S., Piotrovich A.V., Malysheva L.J., Artyukhevich T.V., Kuzmina E.V.

Abstract

Currently, prosthetics based on dental implants is widely used for the replacement of missing teeth, and consequently the number of complications associated with the occurrence of infl ammation surrounding tissues is growing. The object of the research is to conduct analysis the level oral cytokines in the patients with a closed sinus lift with simultaneous dental implantation, ending in the development of early form of periimplants, and to assess the topical immunotherapy effect on concentration saliva cytokines in this implantation method. 105 patients who received implant treatment were comprehensively examined using laboratory and immunological methods – the dynamics of concentrations of IL-1β, IL-1Ra, IL-8, IL-2 and IL-10 of saliva on the 6th, 12th and 24 th day of the postoperative period was evaluated. Based on the analysis of the salivator level of cytokine in patients with early periimplants the imbalance of the pro- and anti-infl ammatory cytokines of the oral cavity was diagnosed. The topical cytokine therapy with betaleukin and roncoleukin leveled out the revealed immunological changes and prevented the chronic infl ammatory response in the early postoperative period.
Russian Journal of Immunology. 2019;22(3):1211-1216
pages 1211-1216 views

PSYCHONEUROIMMUNOMODULATORY EFFECTS OF THE ORIGINAL ANTICONVULSANT AT EXPERIMENTAL ALCOHOLISM

Markova E.V., Savkin I.V., Knyazheva M.A., Shushpanova T.V., Novozheeva T.P.

Abstract

An essential link in the pathogenesis of alcohol dependence is a violation of the mechanisms of neuroimmune interaction, which determines the prospect of finding new effective and safe pharmacological correction tools that act on the molecular targets of the ethanol infl uence in the central nervous system and can correct neuroimmune dysregulation. A positive psychoneuroimmunomodulating effect was demonstrated in the experimental alcohol dependence of the original anticonvulsant, which has a modulating effect on the GABA / benzodiazepine receptor complex. The effect was expressed in the reduction of pathological craving for alcohol, stimulation of parameters of exploratory behavior and immune response, which makes the use of anticonvulsant in the therapy of chronic alcoholism promising and can provide a new pharmacotherapeutic approach to the prevention and treatment of this disease.
Russian Journal of Immunology. 2019;22(3):1217-1223
pages 1217-1223 views

THE EFFECT OF STREPTOCOCCUS PYOGENES ARGININE DEIMINASE ON HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS PROLIFERATION AND ACTIVATION MARKERS EXPRESSION

Mammedova J.T., Burova L.A., Malashicheva A.B., Semenova D.S., Freidlin I.S., Starikova E.A.

Abstract

Arginine is an important nutrient involved in the regulation of homeostasis, vascular tone, as well as the development of infl ammation and immune response. Arginine defi ciency caused by bacterial arginine-deiminase can lead to endothelial dysfunction. In the present study, using the Supernatants of Destroyed Streptococcal Cells (SDSCs) of the original strain (S. pyogenes M49-16) and its isogenic mutant with the inactivated gene of arginine-deiminase ArcA (M49-16delArcA), the effect of arginine deiminase on the proliferative activity and expression of adhesion molecules on human umbilical vein endothelial cells (HUVEC) was evaluated. Cell distribution by cell cycle phases and expression of surface molecules were investigated by fl ow cytometry using monoclonal antibodies. It was shown that the original strain SDSC signifi cantly reduced the proliferative activity of HUVEC and increased the expression of adhesion molecules CD62P, ICAM-1 and tissue factor CD142. The SDSC of the mutant strain did not have any effect. In the presence of supernatants of both strains, the expression of VEGFR-2 receptor did not change. The results showed that streptococcal arginine deiminase promotes development of endothelial dysfunction, infl ammation, and coagulation.
Russian Journal of Immunology. 2019;22(3):1224-1231
pages 1224-1231 views

IMMUNOLOGICAL CHANGES IMMUNOLOGICAL CHANGES

Nikonova S.E., Ryabova L.V., Nikushkina K.V., Emelyanov I.V.

Abstract

21 patients were diagnosed with stage II–IV COPD, the smoking experience was 36,9 ± 2,92 years. In this group of patients a study of immunological status was carried out. The immunological study confi rmed the presence of chronic immunological infl ammation in patients with COPD.
Russian Journal of Immunology. 2019;22(3):1232-1234
pages 1232-1234 views

COMPARATIVE ANALYSIS OF THE INFLUENCE OF RECTAL SUPPOSITORIA WITH CUCRUMIN ON THE CONCENTRATION OF IgG AND IgM IN BLOOD SERUM IN EXPERIMENTAL CROHN’S DISEASE

Osikov M.V., Simonyan E.V., Bakeeva A.E., Boiko M.S.

Abstract

The use of anti-infl ammatory drugs (5-aminosalicylic acid (5-ASA), glucocorticoids) for Crohn’s disease (CD) as a basic therapy is limited to a wide range of side eff ects and requires the search of new therapeutic approaches. The aim of the work is to study the eff ect of rectal suppositories of the original composition with 80% spirituous extract of curcumin on the serum concentration of IgG and IgM in experimental CD. The work was performed on 70 Wistar white rats, CD was modeled by rectal administration of 100 μl of a 3% solution of trinitrobenzenesulfonic acid (TNBS), the extract of curcumin in rectal suppositories of the original composition based on polyethylene glycol was used every 12 hours, in the comparison group rectal suppositoria with 5-ASC were used every 12 hours also. The serum concentration of IgG and IgM was determined using specifi c test systems for rats on an immunoassay analyzer on 3, 5 and 7 days of the experiment. It was determined that in experimental TNBS-induced Crohn’s disease in the serum of rats, the concentration of IgG and IgM increases on days 3, 5 and 7 of the experiment. The use of curcumin extract in rectal suppositories of the original composition in experimental CD leads to a decrease in the concentration of IgG and IgM on days 3 and 5 of observation.
Russian Journal of Immunology. 2019;22(3):1235-1238
pages 1235-1238 views

CLINICAL-LABORATORY CHARACTERISTIC OF PATIENTS WITH SYNDROME DIGEORGE

Pashnina I.A., Vlasova E.V., Deryabina S.S.

Abstract

Cases of children (n=34) and adults (n=3) with DiGeorge syndrome were investigated. In 33 of 34 children 22q11.2 deletion was revealed. In 30 children a cardiac malformation was diagnosed, in 12 cases – in combination with other congenital malformations, all children had face dysmorphisms. Young children had more severe physical and speech development delay. All children showed signs of antiinfectious protection disorders: acute and chronic viral and bacterial infections of different localization. Lethal outcomes were observed in four children: three of them – because of sepsis, one – because of hypocalcemic crisis. The decreased level of the T-cells, T-helpers and cytotoxic T-cells absolute count in peripheral blood was observed in 59–68% of children. The decreased count of B-lymphocytes and natural killers was detected less frequently: in 26% and 15% of patients, respectively.
Russian Journal of Immunology. 2019;22(3):1239-1243
pages 1239-1243 views

THE LEVEL OF INTERLEUKIN17 AND INTERFERON γ IN CELL’S CULTURES SUPERNATANTS UNDER STIMULATION BY ANTIBODIES TO CD3 AND CD28 IN CHILDREN WITH ARTHRITIS OF DIFFERENT ETIOLOGY

Pashnina I.A., Krivolapova I.M.

Abstract

Children of 2–17 years old with juvenile idiopathic arthritis (n = 100), unspecified reactive arthropathy (n = 15) and 31 healthy children (controls) were examined. The spontaneous, anti-CD3-stimulated and anti-CD3/CD28-stimulated concentrations of IL-17 and IFN-γ in the cells cultures supernatants were determined by ELISA. The spontaneous IFN-γ level in children with arthritis of various etiologies was higher than in control, while the IFN-γ concentrations in both variants of stimulation in the patients were lower. The spontaneous IL-17 production in the patients and in healthy children was the same, while stimulated synthesis of IL-17 in the groups with arthritis was higher than in the control group.
Russian Journal of Immunology. 2019;22(3):1244-1248
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PARTICIPATION OF PLASMA OF PREGNANT WOMEN DURING FORMATION OF EXTRACELLULAR TRAPS NEUTROPHIL

Savochkina A.Y., Abramovskikh O.S., Myakisheva E.N., Abramovskikh K.A.

Abstract

Pregnancy is a unique phenomenon of the “peaceful coexistence” of the maternal organism and the semi-allogenic fetus. For maintenance of ability to live, growth, progress of an embryo and a fruit in an organism of future mother there are essential variations which concern practically all systems of an organism, including immune system and system of a hemostasis. In work results of an assessment of capacity neutrophils to formation NETs in I a trimester of pregnancy are presented.
Russian Journal of Immunology. 2019;22(3):1249-1253
pages 1249-1253 views

CHARACTERISTIC OF IMMUNOLOGIICAL STATUS AT PATIENTS WITH DIABETES MELLITUS TYPE2 COMBINED WITH ATHEROSCLEROTIC ARTERIAL DISEASE OF LOWER LIMBS

Savchuk K.S., Ryabova L.V., Nikushkina K.V., Baturina I.L.

Abstract

This article shows results of immunological status’ research at patients with diabetes mellitus type 2 (DM2) combined with atherosclerotic artery disease of lower limbs. Depending on presence of coronary artery disease (CAD) two groups of patients were formed up. With the view to validation of immunological changes’ qualities, we detected levels of IgA, IgG and IgM, circulating immune complex and blood serum cytokine’s levels. It’s elucidated, that patients with DM2 without CAD, compared with patients with CAD, have significant high levels of Interleukin 8. There were no high level markers of chronic infl ammation (IgA, IgG, circulating immune complex and pro-infl ammatory cytokines Il-1β and Il-6) detected in groups of interest.
Russian Journal of Immunology. 2019;22(3):1254-1257
pages 1254-1257 views

EXPERIMENTAL EVALUATION OF THE EFFICIENCY OF THE PEPTIDECONTAINING DRUG IN THE TREATMENT OF CHRONIC PERIODONTITIS

Sarkisyan N.G., Chumakov N.S., Grenaderova M.A.

Abstract

This article addresses the problem of treating chronic periodontitis. On the model of chronic inflammation of the gums created in an experimental study on Wistar rats, a method of topical therapy was proposed and evaluated using a peptide containing silicon glycerogel composition, and the composition was also compared with silicon glycerogel. It has been established that, according to the main clinical features, the composition has a good reparative and antimicrobial eff ect, which makes it possible to approach the therapy of chronic periodontitis both in the pathogenetic and etiological aspects. The timing of clinical improvement increased in the group using the composition with an antimicrobial peptide and silicon glycerogel by 57%, compared with the group in which only glycerohydrogel silicon was used.
Russian Journal of Immunology. 2019;22(3):1258-1262
pages 1258-1262 views

PSYCHOPHYSIOLOGICAL INDICATORS IN MICE RECIPIENTS AFTER MODULATED IMMUNE CELLS TRANSPLANTATION

Serenko E.V., Knyazheva M.A.

Abstract

This article deals with the problem of the regulatory relationships of the main adaptive systems of the body, immune and nervous. Increasing evidence suggests that the immune system is able to have a coordinating infl uence on the behavior and emotional responses of animals and humans. Exploratory behavior is one of the most important types of behavior, which provides the individual with knowledge of the environment and is an essential psychological mechanism of adaptation of higher vertebrates. The purpose of the article was to analyze the psychophysiological indicators in experimental animals after transplantation of immune cells, the functional activity of which was in vitro modulated by the treatment with a psychoactive substance. It was demonstrated that in recipient mice after transplantation of modulated with aminazine immune cells exploratory behavioral editing is recorded against the background of cytokines IL-1ß, TNFα, IL-6 increase in the brain; as well as a decrease in the intensity of the immune response. The presented results show the possibility of using immune cells as model objects for infl uencing the neuroimmune functional relationship; as well as the promising application of cellular technologies for behavioral patterns editing.
Russian Journal of Immunology. 2019;22(3):1263-1268
pages 1263-1268 views

THE STABILITY OF NEOEPITOPES RECOGNIZED BY REGULATORY RHEUMATOID FACTOR AFTER REMOVING THE MODIFYING AGENT REQUIRED FOR THEIR FORMATION

Sidorov A.Y., Korenchuk Y.V., Beduleva L.V., Menshikov I.V., Terentiev A.S., Khramova T.V.

Abstract

Fc fragments of IgG exposing neoepitopes recognized by the regulatory rheumatoid factor have the ability to suppress autoimmune reactions and reduce symptoms of rat collagen-induced arthritis. To induce neoepitopes in Fc fragments of IgG a modifying agent is used. This modifying agent can cause undesirable reactions when introduced into the body. Therefore it is necessary to remove it after Fc fragments modifying. The aim of the study was to fi nd out whether neoepitopes recognized by the regulatory rheumatoid factor remain on Fc fragments of IgG after removal of the modifying agent. It has been found that Fc IgG fragments can be purifi ed from a modifying agent by size exclusion chromatography on a Superdex 200. Removal of the modifying agent leads to the loss of neoepitopes recognized by the regulatory rheumatoid factor on Fc IgG fragments. The loss of neoepitopes after the removal of the modifying agent is accompanied by the oxidation of the sulfhydryl groups of the hinge region which were present on Fc fragments carrying the neoepitopes. This fact suggests that in order to preserve on the Fc fragments of IgG neoepitope recognized by the regulatory rheumatoid factor the reduced state of cysteine residues of the hinge region must be maintained and the modifying agent used creates the necessary conditions for this.
Russian Journal of Immunology. 2019;22(3):1269-1273
pages 1269-1273 views

ESTIMATION OF IMMUNOLOGICAL EFFICIENCY OF PEOPLE VACCINATED AGAINST ANTHRAX

Silkina M.V., Kartseva A.S., Kalmantaeva O.V., Rogozin M.M., Firstova V.V., Shemyakin I.G.

Abstract

Vaccine prevention of anthrax in Russia is carried out according to epidemiological indications 1 time per year with the use of live anthrax vaccine. The main problem is the lack of a method to control the immunological efficacy of vaccination. The article presents data that analyzes the anthrax human humoral and cellular immunity among donors vaccinated 10–12 months ago with live anthrax vaccine. It was shown that in the blood serum of the most vaccinated donors did not detect antibodies to the protective antigen (PA). However, in all vaccinated patients (with the exception of one donor) T and B lymphocytes circulated in the blood enhanced proliferative activity ant the expression of activation receptors on their cell surface in response to PA in vitro. The obtained data suggested that predominantly specific cellular immunity is persisted in the long-term after vaccination against anthrax.
Russian Journal of Immunology. 2019;22(3):1274-1279
pages 1274-1279 views

STUDY OF THE CONTENT OF IgG AND IgM LEVEL IN EXPERIMENTAL ULCER AND IN THE CONDITIONS OF RECTAL SUPPOSITORIES WITH VITAMIN D3

Simonyan E.V., Boyko M.S.

Abstract

Basic therapy of ulcerative colitis (UC) includes 5-aminosalicylic acid derivatives (5-ASA), glucocorticoids, and other drugs that have side effects that limit their use, therefore, the search for and justification of the use of new drugs for pharmacotherapy is necessary. The aim of the work is to study the effects of rectal suppositories with vitamin D3 on serum IgG and IgM concentrations in experimental UC. Work performed on 70 Wistar rats. UC was modeled in two stages: 1 – skin sensitization of an animal with a 3% oxazolone solution, 2 – rectal administration of 3% oxazolone. An aqueous solution of vitamin D3 in the composition of rectal suppositories of the original composition based on polyethylene glycol was used every 12 hours; in the comparison group, rectal suppositories with 5-ASA were used in the same way. The serum concentration of IgG and IgM was determined by ELISA using specific test systems on days 2, 4 and 6 of the experiment. The work revealed a statistically significant decrease in the concentration of immunoglobulins in terms of the use of rectal suppositories with vitamin D3 on days 4 and 6 of experimental ulcerative colitis.
Russian Journal of Immunology. 2019;22(3):1280-1283
pages 1280-1283 views

FEATURES OF CYTOKINE PRODUCTION EX VIVO IN CHILDREN UNDER STRONTIUM EXPOSURE

Starkova K.G., Otavina E.A.

Abstract

The features of spontaneous and induced synthesis of cytokines ex vivo in children under strontium exposure were analyzed. The levels of spontaneous, mitogen-induced (phytohemagglutinin 4 μg/ml, concavalin A 4 μg/ml, lipopolysaccharide 2 μg/ml) and strontium (0.01 mg/ml) cytokine production in supernatants of whole peripheral blood of children living in the endemic area of stable strontium exposure were determined by enzyme immunoassay. When compared to the spontaneous production of cytokines by peripheral blood cells of children in the observation group, we revealed a significant decrease in IL-12 levels by 3.1 times, TNF-α by 5.6 times regarding the comparison group and the absence of differences in the spontaneous synthesis of IL-6, IL-10, IL-17, GM-CSF. Under the conditions of inducing effect of strontium, a decrease in production of TNF-α, IL-6, IL-12, IL-17, GM-CSF was found to be by 5.0, 1.7, 2.4, 1.6, 5.0 times, respectively, at the overall stimulating effect of the metal on the indicators of the observation group, IL-6 by 133.1 times, IL-12 by 1.4 times, GM-CSF by 2.2 times, TNF-α by 1.8 times. The data obtained indicate a modulating effect of strontium on cytokine production in whole blood cultures with a predominantly activating effect on mediators of the pro-infl ammatory action of IL-6, IL-12, GM-CSF, TNF-α.
Russian Journal of Immunology. 2019;22(3):1284-1288
pages 1284-1288 views

GENETIC POLYMORPHISM OF TLR2 IN RHEUMATOID ARTHRITIS PATIENTS RUSSIAN POPULATION OF CHELYABINSK REGION

Stashkevich D.S., Burmistrova A.L.

Abstract

The paper presents the results of a study on the frequency distribution of the occurrence of alleles of single nucleotide polymorphism 2258 G A (Arg753Gln) TLR2 in patients with rheumatoid arthritis of the Russian population of the Chelyabinsk region. Genotyping was performed by allele-specific PCR, followed by detection of the results in a 3% agarose gel. It was shown that in patients with RA, the frequency of occurrence of the allele 2258 * A and the heterozygous genotype G/A TLR2 was increased and the frequency of the homozygous genotype on the ancestral allele was reduced. The established significance of single nucleotide polymorphism of 2258 (Arg753Gln) TLR2 for the formation of resistance / susceptibility to RA in the Russians of the Chelyabinsk region is confirmed by the overdominant and dominant inheritance models.
Russian Journal of Immunology. 2019;22(3):1898-1992
pages 1898-1992 views

EFFECT OF PREGNANCY-SPECIFIC β1-GLYCOPROTEIN ON THE DIFFERENTIATION AND PROLIFERATION OF TH17-POLARIZED HELPER T CELLS

Timganova V.P., Bochkova M.S., Kalugina A.P., Khramtsov P.V., Raev M.B., Zamorina S.A.

Abstract

The effect of the native (non-recombinant) pregnancy specific β1-glycoprotein (PSG) preparation on the differentiation and proliferation of Th-17 polarized helper T cells was studied. The object of the study was the culture of “naive” helper T cells (CD4+) isolated by the immunomagnetic separation. Cells were polarized into the Th17 phenotype using TCR-activator and pro-inflammatory cytokines (IL-1β and IL-6). It was shown that PSG had a suppressive effect on the Th17 cell frequency in culture (CD4+ROR-γt+), and inhibited the proliferation of these cells, as measured by the method of differential gating. Thus, in the experimental model used, PSG had a pronounced suppressive effect on the differentiation and proliferation of T-helpers polarized into the Th17 phenotype.
Russian Journal of Immunology. 2019;22(3):1293-1297
pages 1293-1297 views

THE EFFECT OF THE IMMUNE SYSTEM ON REGENERATIVE PROCESSES OF THE LIVER IN ACUTE TOXIC HEPATITIS

Tуumentseva N.V., Khramtsova Y.S., Artashyan O.S., Gafarova R.K.

Abstract

The immune system plays an important role in the regulation of recovery processes in different tissues and organs, including the liver. Changing the functional state of the immune system with Аzoximeribromidum in acute toxic hepatitis does not remove the signs of toxic damage to the liver, while increasing the migration of lymphocytes to this organ. The drug did not have a significant positive effect on the reparative regeneration of the liver, which may be due to the insufficiently long or delayed in time from the moment of poisoning the drug administration.
Russian Journal of Immunology. 2019;22(3):1298-1303
pages 1298-1303 views

PECULIARITIES OF IMMUNOREGULATION IN ADULTS LIVING UNDER AEROGENIC EXPOSURE TO ALUMINIUM

Chelakova J.A., Guselnikov M.A.

Abstract

A diagnostic examination of 78 people living permanently under the negative impact of emissions resulted from enterprise producing aluminum was carried out. The control group consisted of 52 people. Examination of the adult population revealed changes in the immune system: there is a decrease in the phagocytic activity of cells and IgG deficiency in relation to the norm and control; a significant increase in the relative and absolute level of CD4+CD127 T lymphocytes was observed. What is more, we have seen hyperproduction of antiapoptotic factor Bcl-2 and a decrease in the values of activation markers CD3+CD25+ and CD3+CD95+. At the same time, the level of specific sensitization of the organism to aluminum according to the criterion of immunoglobulin of G class was significantly heightened in comparison with the age norm and the control group. Thus, in adults living under aerogenic exposure to aluminum, changes in cellular immunoregulation characterized by the imbalance of the receptor apparatus of immunocytes and intracellular transcription factors associated with increased specific sensitization of the body to aluminum, which results in slowing of cell death and the formation of hapten-mediated proliferative reactions, were determined. 

Russian Journal of Immunology. 2019;22(3):1304-1307
pages 1304-1307 views

POSTSTRESSOR CHANGES IN FUNCTIONAL ACTIVITY OF CELLS OF PERITONEAL CAVITY OF MICE

Sharav’eva I.L., Gein S.V.

Abstract

The effect of acute and chronic cold stress in the conditions of the blockade of opiate receptors on the oxidative activity of mouse peritoneal macrophages was studied. It was established that acute cold stress (4°C, 4 hours), regardless of the blockade of opiate receptors, inhibits zymosan-induced oxidative activity of mouse peritoneal macrophages. Chronic cold stress (4°C, 4 hours, for 7 days) on the 7th day activates zymosan-induced oxidative activity of macrophages, blockade of opiate receptors leads to the cancellation of this stimulation. An increase in the concentration of corticosterone is observed both in acute and in chronic hypothermia and does not depend on the blockade of opiate receptors.
Russian Journal of Immunology. 2019;22(3):1308-1312
pages 1308-1312 views

ANTIMICROBIAL EFFECT OF SOME COMPOUNDS OF PLATINUM AND ONE OF PYRIDINIUM DERIVATIVES IN RELATION TO ESCHERICHIA COLI

Shlepotina N.M., Kolesnikov O.L., Shishkova Y.S., Galagudin I.V., Kalita E.V., Tkachyova A.R., Kim D.G.

Abstract

At present, the resistance to antibacterial substances being formed in microorganisms opens up new areas of search for alternative compounds with similar properties. In scientific research in recent years, the antimicrobial properties of platinum compounds and pyridinium derivatives have been actively studied. The purpose of this study was to study effect against Escherichia coli of aqueous solutions of 1-allyl-2- aminopyridinium bromide and two hexachloroplatinate complexes in three experiments. As a result of the carried out research, a reliable antibacterial effect of one of the platinum compounds was revealed in relation to Escherichia coli.
Russian Journal of Immunology. 2019;22(3):1313-1318
pages 1313-1318 views


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