Vol 22, No 2-1 (2019)

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Full Issue

ORIGINAL ARTICLES

EFFICIENCY OF HYPOXIC THERAPY AT SUBCLINICAL THYROID DYSFUNCTION WITH AUTOIMMUNE GENESIS

Abazova Z.K., Borukaeva I.K., Kumykov V.K., Shibzukhova L.A.

Abstract

The efficiency of hypoxic therapy in treatment of subclinical thyroid dysfunction with autoimmune genesis is shown. After treatment the immunological parameters, which changes led to formation of thyroid dysfunction, normalized. An indicator of recovery after treatment was the normalization blood levels of pituitary thyroid-stimulating hormone.

Russian Journal of Immunology. 2019;22(2-1):124-126
pages 124-126 views

INFLUENCE OF OZONOTHERAPY ON IMMUNE STATUS AT ISOLATED FRACTURES OF THE FEMORAL BONE

Abramov K.S., Davydova E.V., Osikov M.V., Kochengina S.A.

Abstract

The study of the dynamics of the immune system parameters in men of working age with an isolated fracture of the femur in the early post-traumatic period showed the formation of an immunosuppressive background, due to an increase in the circulation of T-regulatory cells. The use of ozone therapy in the form of small autohemotherapy reduces the manifestations of immunosuppression by reducing the number of T-regulatory cells in the bloodstream against the background of an increase in the number of natural killer cells, which will make it possible to reduce the number of infectious complications.

Russian Journal of Immunology. 2019;22(2-1):127-129
pages 127-129 views

INFLUENCE OF OZONOTHERAPY ON IMMUNE STATUS AT ISOLATED FRACTURES OF THE FEMORAL BONE

Abramov K.S., Davydova E.V.

Abstract

The study of the functional activity of blood monocytes under conditions of ozone therapy for isolated femoral fractures showed that post-traumatic femoral fractures are accompanied by functional activation of cells of the innate compartment of the immune system in the form of increased adhesive and bactericidal activity. The use of ozone therapy in the form of a small autohemotherapy procedure increases the level of monocyte functional activity, contributing to the activation of wound cleansing processes and the prevention of infectious complications.

Russian Journal of Immunology. 2019;22(2-1):130-131
pages 130-131 views

MEDICINES OF MONOCLONAL ANTIBODIES IN ONCOLOGY

Avdeeva Z.I., Soldatov A.A., Kiselevskiy M.V., Bondarev V.P.

Abstract

The medicines of monoclonal antibody which are successfully applied in clinical practice to treatment of oncological diseases are result of scientific achievements of biotechnology developments and researches in the field of molecular immunology. However, despite efficiency of use of the specified biotherapeutic drugs, remains many unresolved questions evolving when developing new medicines from recombinant proteins and at their clinical use. It is connected with the choice of a target antigen taking into account carcinogenesis mechanisms when developing medicine, comprehensive assessment of qualitative characteristics of recombinant protein, providing appropriate conditions of production, assessment of safety of a clinical use connected, first of all, with manifestations of «undesirable» immunogenicity of medicine.

Russian Journal of Immunology. 2019;22(2-1):133-135
pages 133-135 views

IMMUNOLOGICAL CORRELATES OF A CANDIDATE DNA VACCINE AGAINST HIV-1 SUBTYPE A

Akulova E.B., Murashev B.V., Nazarenko O.V., Verevochkin S.V., Masharsky A.E., Al Shekhadat R.I., Lioznov D.A., Kozlov A.P.

Abstract

Since 1981, when AIDS was identified as a disease about 60 million of HIV infected people have been registered and 30 million have died according to WHO. The development of vaccines against HIV/AIDS is an important step in solving this problem. In the absence of an adequate animal model for HIV, vaccine development requires an understanding of immunological correlates of HIV protection. In this study we investigated the immunological correlates of a candidate HIV-1 DNA vaccine. We analyzed the specific immune responses in phase I clinical trial participants and in HIV exposed seronegative individuals. In both groups, an increase in TNFα cytokine by CD4+ T cells in response to specific peptide stimulation was found. This could be an indicator of the possible vaccine efficacy.

Russian Journal of Immunology. 2019;22(2-1):136-138
pages 136-138 views

MICROVESICLES IN FOLLICULAR FLUID WITH WOMEN IN IVF PROGRAM

Andreeva E.A., Khonina N.A., Pasman N.M.

Abstract

Microvesicles has a signifi cant potential as a new diagnostic and prognostic biomarker including reproductive pathologies. In this paper shown that an elevated level of microvesicles in women’s follicular fluid is associated with the age, the duration of infertility and clinical pregnancy.

Russian Journal of Immunology. 2019;22(2-1):139-141
pages 139-141 views

MYELOID-DERIVED SUPPRESSOR CELLS IN MULTIPLE MYELOMA PATIENTSDURING THE MOBILIZATION OF HEMATOPOETIC STEM CELLS

Aristova T.A., Batorov E.V., Ostanin A.A.

Abstract

We investigated the relative content of circulating early (LinCD33+HLA-DR–CD66b), granulocyte (LinCD33+HLA-DR–CD66b+) and monocyte (CD14+HLA-DRlow/–) myeloid-derived suppressor cells (MDSCs) in patients with primary multiple myeloma (MM) at the mobilization of hematopoietic stem cells (HSC). The relative count of all the studied subtypes of MDSCs in patients with both complete remission and partial response did not significantly differ from those in healthy donors. Mobilization of HSCs was not associated with any changes in the content of CD14+HLA-DRlow/– and LinCD33+HLADR–CD66b, however, led to a significant increase in Lin–CD33+HLA-DR–CD66b+. Thus, carrying out the mobilization of HSCs in patients with MM maybe accompanied by the accumulation of granulocyte MDSCs in the autograft. 

Russian Journal of Immunology. 2019;22(2-1):142-143
pages 142-143 views

HIV INFECTION AND HIV/HCV CO-INFECTION PROBLEM

Balmasova I.P., Malova E.S., Efratova E.P.

Abstract

The patterns of coinfection, which extend to coinfection of HIV/HCV, make it expedient to test the hypothesis about the impact of the order of viral pathogens admission into host for the development of the infectious process. It was found that this factor has a significant impact on the course of liver fibrosis, the viral load of HIV and HCV, the state of the immune response. In cases where HIV infection develops before hepatitis C virus infection, liver fibrosis often takes a progressive course, the viral load of HIV is reduced, and HCV load increases, CD8+ T-lymphocytes and immunoregulatory index values become lower.

Russian Journal of Immunology. 2019;22(2-1):144-146
pages 144-146 views

IMMUNOLOGICAL PARAMETERS IN PSORIASIS DEPENDING ON THE DISEASE SEVERITY

Barilo A.A., Smirnova S.V.

Abstract

Psoriasis is one of the most common skin diseases and occupies one of the leading places among the medical and social problems of modern dermatology. The results of a comparative analysis of the quantitative characteristics of T- and B-lymphocytes, phagocytic cells in peripheral blood, the concentration of immunoglobulins (A, M, G), circulating immune complexes (CIC-C1q, CIC-C3d) and features of changes in immunological parameters depending on the severity of psoriasis were determined. The features of changes in immunological parameters in psoriasis, depending on the severity.

Russian Journal of Immunology. 2019;22(2-1):147-149
pages 147-149 views

FEATURES OF THE SPECTRUM OF FOOD SENSITIZATION WITH ATOPIC BRONCHIAL ASTHMA IN CHILDREN

Barilo A.A., Borisova I.V., Smirnova S.V.

Abstract

Bronchial asthma is one of the most common chronic diseases of broncho-pulmonary system affecting all age groups, and is a global public health problem. The prevalence of food allergies among patients with asthma varies widely, which determines the relevance of the study. The results of studying the spectrum of sensitization to food allergens are given, and the peculiarities of food allergy in children with bronchial asthma living in the Krasnoyarsk Territory are determined.

Russian Journal of Immunology. 2019;22(2-1):150-152
pages 150-152 views

MOLECULAR COMPONENT-RESOLVED DIAGNOSTICS FOR CAT ALLERGY IN CHILDREN WITH ALLERGIC RHINITIS

Bass E.A.

Abstract

Hypersensitivity to cat allergens is a serious clinical problem in many countries because of the high prevalence of these animals. The aim of this study is to assess the spectrum of sensitization to the cat allergen’smolecular components and its role in allergic rhinitis in children. IgE-aB to rFel d 1 were detected in 91.8% of children, to nFel d 2 ‒ in 16.4%, to rFel d 4 ‒ in 43.8% of patients. At the same time ‒ 56.2% of children were monosensitizated to rFel d 1; 27.4% with identifi ed IgE-aB to rFel d 1 and rFel d 4; 8.2% ‒ from IgE-aB to rFel d 1 + nFel d 2 + rFel d 4; and 8.2% ‒ with IgE-aB to nFel d 2 + rFel d 4. Sensitization only to minor allergens did not lead to the development of asthma, but exacerbations of AR in these patients occurred more frequently. To assess the prognosis of the disease and possible reactions to cross-allergens, we recommend component-resolved diagnostics.

Russian Journal of Immunology. 2019;22(2-1):153-155
pages 153-155 views

ABILITY OF CONDITIONALLY-PATHOGENIC MICROORGANISM TO INACTIVATE ANTI-MICROBIAL PROTEIN BY LYSOZYM UNDER THE INFLUENCE OF METABOLITES ASSOCIATION OF BIFIDOBACTERIA

Bekpergenova A.V., Perunova N.B., Andryushenko S.V., Zdvizhkova I.A., Mironova A.R.

Abstract

Materials on the eff ect of the supernatants of the associations of bifi dobacteria on the antilysozyme activity of microorganisms are presented. An increase in the antilysozyme activity was detected in the lactosopositive non-hemolytic strains of E. coli isolated during human intestinal eubiosis, and, conversely, a decrease in hemolytic E. coli, S. aureus and C. albicans isolated in dysbiosis. The available materials contribute to the study of the mechanisms of formation of microsymbiosis in the associative symbiosis of humans and contribute to the identifi cation of strains of bifi dobacteria, suitable as pro- and metabiotics.

Russian Journal of Immunology. 2019;22(2-1):156-158
pages 156-158 views

ROLE OF GENE POLYMORPHISM OF VITAMIN D RECEPTOR (VDR FokI) IN THE DEVELOPMENT OF PULMONARY TUBERCULOSIS IN RUSSIAN CHELYABINSK REGION

Belyaeva S.V., Stashkevich D.S.

Abstract

For the fi rst time the analysis of the VDR gene in Russian patients with pulmonary tuberculosis living in Chelyabinsk region has been carried out. It is established that the frequency of occurrence of allele FokI(f) in patients does not diff er from the control. A genotype FokI(f/f) was not found in patients with a focal form.

Russian Journal of Immunology. 2019;22(2-1):159-161
pages 159-161 views

SOME BIOLOGICAL FEATURES OF ANTI-IDIOTIPIC AND TERTIARY ANTIBODIES AGAINST TWO MORPHINE DERIVATIVES

Berzina A.G., Ulyanova L.I., Gamaleya N.B., Klimova T.A., Trofimov A.V., Stankova N.V.

Abstract

The horse anti-idiotypic antibodies (Ab2) were obtained on the base of 2 mouse monoclonal antibodies (mAb) specific for two morphine derivatives – 3-0-carboxymethyl (CMM) and 6-hemisuccinyl (GSM). Immunization of rabbits and mini-pigs with Ab2 led to the formation of tertiary antibodies (Ab3). It was found that Ab2 antibodies have morphine-like properties, i. e. stimulate DNA synthesis in cultured human glioblastoma cells of the T98G line, which expresses a significant amount of opioid receptors on cell membrane. The addition of Ab3 antibodies to the System in vitro led to the abolition of the stimulating effect.

Russian Journal of Immunology. 2019;22(2-1):162-164
pages 162-164 views

USE OF STATINS FOR THE TREATMENT OF NON-INFECTIOUS UVEITIS

Biryukova A.A., Shirinsky I.V.

Abstract

Inhibitors of inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase or statins, are used for the treatment of cardiovascular diseases and dyslipidemia. Immunomodulating and anti-infl ammatory properties of statins provide a rationale for their use in various autoimmune diseases including noninfectious uveitis. In this article we present literature data on the use of statins in the experimental models of non-infectious uveitis and on the epidemiological studies showing prophylactic eff ect of statins against uveitis. In our pilot, open-label, randomized study we demonstrated effi cacy and safety of treatment of uveitis with simvastatin in addition to conventional treatment. In conclusion, current data provide a rationale for a randomized, placebo controlled study of statins in uveitis.

Russian Journal of Immunology. 2019;22(2-1):165-167
pages 165-167 views

ANALYSIS OF IMMUNOLOGICAL INDEXES AND EXTERNAL BREATH FUNCTION IN PATIENTS WITH BRONCHIAL ASTHMA UNDER THE TREATMENT WITH T-CELL IMMUNOTHERAPY AND STANDARD THERAPY

Blinova E.A., Makarova A.E., Pashkina E.A., Demina D.V., Kozlov V.A.

Abstract

The study presents the clinical and immunological characteristics of patients with bronchial asthma, which had administrated with standard therapy and T-cell immunotherapy. After cell therapy, it was shown an improvement of external breath function, an increase in the number of CD8+ lymphocytes, a decrease in CD19+ cell subset, as well as a reducing of IgE level in the atopic form of the disease. All together it indicates the eff ectiveness of cell therapy. In patients with standard therapy, there was no change of investigated parameters in dynamics.

Russian Journal of Immunology. 2019;22(2-1):168-170
pages 168-170 views

ANALYSIS OF THE CAUSES OF EMERGIND AND PECULIARITIES OF THE DIAGNOSTICS OF ALLERGIC DISEASES IN CHILDREN LIVING ON THE TERRITORY OF THE KURSK REGION

Bogomazov A.D., Zaitseva L.Y., Seryozhkina A.S.

Abstract

The growth of allergic diseases in the world attracts more and more specialists to this problem. According to the work carried out at the State Research Center – Institute of Immunology, Ministry of Health of the Russian Federation, the prevalence of allergic diseases in diff erent regions of Russia ranges from 15.0 to 35.0%. In our study, conducted on the territory of the Kursk region, we studied the causes of, as well as the features of the diagnosis of atopic diseases in children.

Russian Journal of Immunology. 2019;22(2-1):171-173
pages 171-173 views

EFFECT OF INTERVAL HYPOXYTHERAPY AND ENTЕRAL OXYGENOTHERAPY ON IMMUNOLOGICAL REACTIVITY IN PATIENTS WITH BRONCHAL ASTHMA

Borukaeva I.K., Abazova Z.K., Ragimbekova M.R., Temirzhanova F.K.

Abstract

Bronchial asthma is a classic, antigen-specific disease, in the formation of which the reagin-dependent type of allergic reaction is crucial. The combined use of interval hypoxitherapy and enteral oxygen therapy has proven to be an eff ective method for improving immunological reactivity in patients with bronchial asthma. A significant increase in the content of IgA in the blood led to increased antimicrobial and antiviral immunity. The decrease in the content of IgE in the blood can be seen as a manifestation of a decrease in the chronic infl ammatory process in the bronchial tree of patients and an increase in anti-infective immunity. A significant increase in the content of T-lymphocytes led to an improvement in cellular and humoral immunity and a decrease in immuno-regulatory index.

Russian Journal of Immunology. 2019;22(2-1):174-176
pages 174-176 views

IMMUNORABILITATION IN CHRONIC ISCHEMIA OF THE BRAIN

Bystrova N.A., Shulginova A.A., Sunyaykina O.A., Mashoshina D.O.

Abstract

We examined 26 patients of the neurological department of the BMU “Kursk Regional Clinical Hospital” with chronic cerebral ischemia stage II against the background of hypertensive disease stage II. All were divided equally by random randomization and within two weeks received a comprehensive basic pharmacological therapy (enalapril, vinpocetine, ceraxon and mexicor). Half of the patients additionally received polyoxidonium. Immune disorders were assessed by indicators in the peripheral blood of the functional metabolic activity of neutrophils, plasma levels of cytokines, complement components and their inhibitors. Prior to treatment, violations indicating the presence of immune infl ammation were identifi ed. Inclusion of polyoxidonium into the complex pharmacotherapy eff ectively corrects the altered immune status parameters.

Russian Journal of Immunology. 2019;22(2-1):177-179
pages 177-179 views

LABORATORY POSSIBILITY IN THE DIAGNOSIS OF DRUG HYPERSENSITIVITY

Bychkova N.V.

Abstract

Drug hypersensitivity reactions are not very common in the population, but can be a serious problem for patients and physicians. Diagnosis of this disorder should consist of a complex of clinical and laboratory methods. A promising method of in vitro diagnostics is the basophil activation test. The study showed the high diagnostic significance of this test in determining hypersensitivity to radiocontrast media and local anesthetics. The presence of in vitro hypersensitivity to drugs can be latent in nature and manifest itself clinically only if the regulatory capabilities are impaired.

Russian Journal of Immunology. 2019;22(2-1):180-182
pages 180-182 views

FACTORS OF IMMUNOENDOCRINE REGULATION IN ALCOHOL DEPENDENCE AT THE STAGE OF FORMATION OF THERAPEUTIC REMISSION

Vetlugina T.P., Nikitina V.B., Mandel A.I., Boiko A.S., Prokopieva V.D., Bokhan N.A.

Abstract

The aim of the study was to study the dynamics of the immunoendocrine factors in patients with alcohol dependence in the process of therapy of withdrawal syndrome and post-withdrawal state. In 35 men with alcohol dependence in the dynamics of formation of therapeutic remission, the level of spontaneous production of spectrum of cytokines by immunocompetent cells was investigated; in the blood serum – hormones. Control – 19 practically healthy men. Hyperproduction of proinfl ammatory cytokines, increase in the concentration of cortisol, decrease in the concentration of T3 and T4 were established. Dependence of IFNγ, IL-1ra, IL-2 and testosterone on character of remission was revealed that allows considering these factors as candidates into biomarkers of prediction of the stability of remission.

Russian Journal of Immunology. 2019;22(2-1):183-186
pages 183-186 views

THE ROLE OF CD4+CD25+CD127– T CELLS AND CYTOKINE RESPONSE IN THE REGULATION OF SYSTEMIC INFLAMMATION IN CHRONIC OBSTRUCTIVE PULMONARY DISEASE

Vitkina T.I., Lysak A.A., Sidletskaya K.A.

Abstract

The regulatory mechanisms of systemic infl ammation in chronic obstructive pulmonary disease (COPD) are not well understood. The aim is to establish the features of immunosuppressive reactions in COPD patients. The study included 112 patients with stable mild-to-severe COPD and 32 healthy volunteers. Cytokine levels (IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α, IFN-γ) and T-regulatory cell (Treg) number were determined using fl ow cytometry. A decrease in Treg number has been foundinsevere COPD. The mechanism of this process may be their competitive interaction with T helper (Th) 17 cells, which is mediated by IL-6action. Ultimately, it contributes to Th17/Treg disbalance and the switching of Th1 immune response to Th17, leading to worsening COPD.

Russian Journal of Immunology. 2019;22(2-1):187-189
pages 187-189 views

THE EXPRESSION OF DIFFERENT ISOFORMS OF TGFβ AND ITS RECEPTORS IN UTERINE LEIOMIOMA

Voronin D.N., Voskresenskaya D.L.

Abstract

The distinctive feature of the myomatous node tissue is the increased reception of TGFbeta. The main role in the activation of fi broblasts and their production of collagen I type in the myomatous node plays TGFbeta1, TGFbet2 and TGFbeta3. The higher level of synthesis and production of TGFbeta1 isoform in the nodes with large amount of collagen I type allow to suggest the leading role of this cytokine in the regulation of the intensity of fi brosis in the leiomyoma tissue.

Russian Journal of Immunology. 2019;22(2-1):190-191
pages 190-191 views

RELATIONSHIP CHARACTER OF COLLAGEN SYNTHESIS IN UTERINE LEIOMYOMA TISSUE WITH FEATURES OF TUMOR GROWTH

Voskresenskaya D.L., Malyshkina A.I., Antsiferova Y.S.

Abstract

The aim of the work was to elucidate the features of collagen 1A1 mRNA expression in uterine leiomyoma. It was found that the high level of collagen synthesis is associated with rapidly increase of leiomyoma size, more frequently noted in small tumors with minimal proliferative activity. Thus, the activity of tissue fi broblasts defi nes the features of tempo and character growth of uterine leiomyoma.

Russian Journal of Immunology. 2019;22(2-1):192-193
pages 192-193 views

ANTIPATERNAL ANTI-LEUKOCYTE ANTIBODIES AS MARKERS OF THE EFFICACY OF ALLOIMMUNIZATIONIN WOMEN WITH RECURRENT PREGNANCY LOSS

Vtorushina V.V., Krechetova L.V., Tetruashvili N.K., Nikolaeva M.A.

Abstract

The indicator of efficacy of lymphocyte immunotherapy (LIT) in women with recurrent pregnancy loss(RPL) is the level of antipaternal anti-leukocyte antibodies (APAB) detected by method of flow cytometry. The article presents the results of determining APAB in the dynamics of pregravid preparation and pregnancy in women with RPL and pregnancy outcomes after LIT.

Russian Journal of Immunology. 2019;22(2-1):194-196
pages 194-196 views

FUNCTIONAL POLYMORPHISMS OF THE TNFα (G-308A), IL-1β (T-31C), IL-10 (C-592A) GENES AS FACTORS AFFECTING THE EFFECT OF CHEMOTHERAPY FOR ADVANCED OVARIAN CANCER

Gening S.O., Abakumova T.V., Antoneeva I.I., Gening T.P., Dolgova D.R.

Abstract

The paper discusses the role of a genetically determined imbalance in the cytokine network in assessing the eff ectiveness of platinum-containing chemotherapy in patients with serous ovarian adenocarcinoma. The frequency of functional polymorphisms of the cytokine IL-1β (T-31C), TNFα (G-308A), IL-10 (G-592A) genes was determined by qRT-PCR in control and in patients with advanced ovarian cancer. We studied the frequency distribution of alleles and genotypes of the IL-1β, TNFα, IL-10 genes in patients with OC in groups with relapses within a year after platinum-containing chemotherapy and without recurrence. The alleles -308G TNFα, -31T IL-1β, -592A IL-10 were found to be associated with disease progression or recurrence within 1 year after completion of treatment.

Russian Journal of Immunology. 2019;22(2-1):197-199
pages 197-199 views

SUBPOPULATIONS OF MYELOID DERIVED SUPRESSORS CELLS IN CHILDREN WITH PSORIASIS

Gerasimova D.G., Radygina T.V., Zakirov R.S., Ambarchyan A.I., Materikin A.I., Epishev N.N., Murashkin A.I., Petrichuk S.V.

Abstract

The absolute and relative number of myeloid derived suppressor cells, regulatory T-lymphocytes and Th17-lymphocytes were assessed in 115 patients with psoriasis and 53 healthy children aged 1.5 to 18 years. An increase in the number of MDSCs due to G-MDSCs was found, depending on the duration of the disease. As the severity of the disease increases, the relative number of M-MDSCs increases. The number of MDSCs directly correlates with the number of Th17 lymphocytes and Treg.

Russian Journal of Immunology. 2019;22(2-1):200-201
pages 200-201 views

A MONOCHROMATIC INCOHERENT LIGHT OF THE450 NM OPTICAL RANGE AFFECTS THE CONTENT OF CYTOKINES IN THE MAXILLARY WASHES IN RHINOLOGIC PATIENTS

Gizinger O.A., Ziganshin O.R., Laknitskaya A.O., Karandashov V.I., Zavgorodniy E.S.

Abstract

The data on the state of the functional metabolic status-neutrophilic granulocytes in washes from the skin surface aff ected by S. aureus after therapy with incoherent monochromatic light with a wavelength of 450±10 nm are presented. The results of the study showed that in patients with staphylococcal lesions of the skin a violation of phagocytic activity, oxygen-dependent metabolism in the NBT-test is recorded. The use of light with a wavelength of 450±10 nm helps to reduce the infl ammatory response, edema, and restore the functional potential of neutrophilic granulocytes.

Russian Journal of Immunology. 2019;22(2-1):203-205
pages 203-205 views

MORPHOLOGICAL CHANGES IN ENDOMETRY IN INFERTILE WOMEN UNDER CHRONIC INFLAMMATION

Godovalov A.P., Toropitsyn S.A., Karpunina T.I.

Abstract

In the development of infertility, the role of chronic inflammatory diseases that are involved in the formation of the morphological basis for fertility decline is being actively considered. In the present study, a retrospective analysis of the results of the histological examination of the pipeline biopsy specimens of 64 women with an established diagnosis of chronic endometritis was carried out. The presence of cell inf ltration was shown in 67% of cases, as well as a combination of dystrophic and proliferative processes (33%), which in general may indicate the presence of persistent infection.

Russian Journal of Immunology. 2019;22(2-1):206-207
pages 206-207 views

THYMUS IN AUTOIMMUNE DISEASES CLINIC. THE ACTUALITY AND PROSPECTIVES OF “IN VIVO” MONITORING

Goloviznin M.V., Lakhonina N.S., Timofeev V.T., Buldakova Y.R.

Abstract

The leading role of Thymus in adoptive immunity development was established 50 years ago. Nevertheless T-cells immunity monitoring in clinical immunology is now testing of growing number of “immune periphery” items disregarding thymus as a central T-immunity organ. We showed that T-cells rearrangement excision circles (TREC) could be the important chain in thymus and peripheral T-cells function assessment in adult and aged healthy donors and rheumatoid arthritis patients. It will create the possibility to form more complex “in vivo” monitoring of thymus activity in autoimmunity.

Russian Journal of Immunology. 2019;22(2-1):208-211
pages 208-211 views

BIOFLAVONOIDS EFFICIENCY IN EXPERIMENTAL ALCOHOLISM

Goldina I.A., Markova E.V., Savkin I.V.

Abstract

The prolonged alcohol consumption has a negative effect on the immune and nervous systems, causing a breakdown in their functional connections. The purpose of this work was to study the effect of turmeric bioflavonoids on the behavior and the cellular immune response in animals in a state of experimental alcoholism. It was found that in chronic ethanol intoxication, the use of turmeric extract led to the stimulation of motor and exploratory activity in the «open field» test and an increase in the cellular immune response to the level peculiar to that of healthy animals of a corresponding age, indicating a positive effect of turmeric in experimental alcoholism.

Russian Journal of Immunology. 2019;22(2-1):212-214
pages 212-214 views

OBTAINING OF MONOCLONAL ANTIBODY FOR IMMUNOAFFINITY PURIFICATION OF MYELOPEROXIDASE AND PROTOTYPE OF HEMOSORBENT

Gorbunov N.P., Kostevich V.A., Sokolov A.V.

Abstract

Myeloperoxidase (MPO) is a promising target for anti-inflammatory therapy and prevention of atherosclerosis. It is known that MPO is an independent predictor of complications of cardiovascular pathologies, including secondary myocardial infarction. For developing an enzyme-linked immunoassay, we obtained monoclonal antibodies against MPO (clone 2F7), which, interacted with MPO according to the electrostatic principle according to the results of surface plasmon resonance analysis. When antibodies were immobilized on cyanogen bromide-activated agarose, the resulting sorbent selectively bound MPO at 0.15 M NaCl. The elution of MPO was provided by 1 M NaCl (pH 7.4), which did not disrupt the properties of the prosthetic heme group. When analyzing the sorption of MPO from plasma samples, the dissociation constant about 0.07 nM was determined. Given that the pathological concentration of MPO exceeds 0.15 nM, the resulting sorbent can be a prototype of a hemosorbent for MPO.

Russian Journal of Immunology. 2019;22(2-1):215-217
pages 215-217 views

STUDYING OF THE ASSOCIATION BETWEEN HLA-G GENE POLYMORPHISM AND RISK RECURRENT MISCARRIAGE IN WOMEN WITH PERSISTENT BACTERIAL-VIRAL INFECTION

Gordeeva L.A., Voronina E.N., Polenok E.G., Mun S.A., Olennikova R.V., Filipenko M.L., Glushkov A.N.

Abstract

The associations between polymorphisms HLA-G (rs41551813, rs12722477, rs41557518 and rs16375) and recurrent miscarriage and risk of recurrent miscarriage (RM) in women with persistent bacterial-viral infection were investigated. It is found that the HLA-G rs12722477 110Ile allele is associated with the risk of RM only in women with persistent bacterial-viral infection (OR=2,49 (1,91–5,21); Pс=0,04).

Russian Journal of Immunology. 2019;22(2-1):218-220
pages 218-220 views

IDIOTYPIC AND ANTI-IDIOTYPIC ANTIBODIES AGAINST POLYCYCLIC AROMATIC HYDROCARBONS IN HUMAN BLOOD SERUM ARE NEW BIOMARKERS OF LUNG CANCER

Grebenshchikov I.S., Studennikov A.E., Glushkov A.N., Ustinov V.A.

Abstract

We studied the levels of idiotypic and anti-idiotypic antibodies against benzo[a]pyrene in the blood serum of lung cancer patients and healthy donors by direct ELISA. It was shown that antibody levels between these groups was statistically signifi cantly different. Based on these results, a quantify antibodies immunoassay in the human serum was developed using a multiplex method.

Russian Journal of Immunology. 2019;22(2-1):221-223
pages 221-223 views

THE LEVEL OF THE INFLAMMATORY AND ANTI-INFLAMMATORY CYTOKINES IN COPROFILTRATES OF THE PATIENTS WITH REACTIVE ARTHRITIS DEPENDING ON THE DEGREE OF INTESTINAL DYSBIOSIS

Danilova E.I., Chelpachenko O.E., Chainikova I.N., Ivanova E.V., Nikiforov I.A.

Abstract

A comparative clinical and immunological examination of children with reactive arthritis (ReA) and healthy revealed an increase in the level of pro-infl ammatory (INF-γ, TNF-α, IL-6) and anti-infl ammatory (IL-10) cytokines in coprofilters depending on the increase in the severity of dysbiotic disorders of the intestinal microbiota, which indicates the possibility of using these criterions as diagnostic and prognostic markers of Re A.

Russian Journal of Immunology. 2019;22(2-1):224-226
pages 224-226 views

IMMUNOGENETIC ASPECTS OF THE FORMATION OF CONGENITAL CONOTRUNCUS WITHOUT CHROMOSOMAL DISEASES

Deeva N.S., Tsepokina A.V., Shabaldin A.V., Shmulevich S.A., Voronina E.N., Filipenko M.L., Shabaldina E.V.

Abstract

The study showed the role of the gene HLA-G 3’UTR in the development of congenital malformations of conotruncus. The study included 14 children with tetralogy of Fallot and 103 healthy children. HLAG 3’UTR typing was performed by amplification of polymorphic gene sites by allele-specific polymerase chain reaction (PCR), in accordance with the manufacturer’s Protocol (Applied Biosystems, USA), with further detection of results in polyacrylamide gel. The study found that in the group of children with a tetralogy of Fallot homozygous genotype HLA-G 3’UTR14-bp ins/ins met statistically significantly more often than in the control group.

Russian Journal of Immunology. 2019;22(2-1):227-229
pages 227-229 views

CONGENITAL AND ADAPTIVE IMMUNITY UNDER EXPERIMENTAL ACUTE PANCREATITIS DUE TO ALCOHOLIC INTOXICATION; CORRECTION OF DISORDERS

Dolgareva S.A., Bushmina O.N., Konoplya N.A., Khorlyakova O.V.

Abstract

In experimental acute destructive pancreatitis due to 60-day-long chronic alcohol intoxication, in comparison with the introduction of ethanol only, more pronounced suppression of adoptive cellular and humoral forms formation of the immune response to sheep erythrocytes, a decrease in the phagocytic capabilities of neutrophils with an increase in their oxygen-dependent activity have been determined. The use of glutoxim, mexidol and heptral has normalized and corrected most of the altered immune parameters.

Russian Journal of Immunology. 2019;22(2-1):230-232
pages 230-232 views

PREDICTOR SIGNIFICANCE OF POLYMORPHISMS OF INTERFERON GAMMA AND INTERLEUKIN4 GENES IN CHILDREN IN BRONCHIAL ASTHMA

Dolgopolov M.S., Prosekova E.V.

Abstract

At present, in spite of a considerable number of studies, the questions of studying the polymorphisms of genes associated with the regulation of immune homeostasis in allergic diseases remain relevant. The aim of the work included the analysis of the frequency of occurrence of variations in the interferongamma and interleukin-4 genotypes in children with asthma and healthy peers. As a result of the study, a signifi cant prevalence of the heterozygous variant of the IL-4 gene was found in the group of healthy children and the peer group with BA, the haplotype CC prevails in healthy peers. When analyzing the genetic variation of the IFNγ gene, a protective eff ect was revealed in the haplotype of TT and the heterozygous variant of AT in relation to the risk of developing bronchial asthma. The results of the study allow us to recommend haplotype TT as a protective marker for the development of allergic BA. The studies established the predictor signifi cance of polymorphisms of the IFNγ and IL-4 genes in children with bronchial asthma.

Russian Journal of Immunology. 2019;22(2-1):233-235
pages 233-235 views

COMPARATIVE ANALYSIS OF THE INDICATORS OF THE FUNCTIONAL ACTIVITY OF NEUTROPHILS PERIPHERAL BLOOD IN PATIENTS WITH SEVERE SEPSIS AND SEPTIC SHOCK

Dolgushin I.I., Savochkina A.Y., Pykhova L.R., Abramovskihk O.S., Chetvernina E.A., Poltorak A.E.

Abstract

A comparative analysis of the functional activity of peripheral blood neutrophils in patients with sepsis, depending on the severity of the disease, has been carried out. An increase in the total number of leukocytes, neutrophil extracellular networks, indicators of neutrophil absorption activity and the activity of intracellular oxygen-dependent metabolism of neutrophils in patients with septic shock was detected.

Russian Journal of Immunology. 2019;22(2-1):236-238
pages 236-238 views

RESULTS OF THE PRE-CLINICAL STUDY OF A NEW IMMUNOCORRECTOR OF FLORAL ORIGIN

Dutova S.V., Karpova M.R.

Abstract

The article presents the results of the preclinical study of the immunocorrective properties of the total ethanol extract from the raw material of the medicinal plant of the traditional medicine of Siberia – Coluria geoides (Pall.) Ledeb.), Rosaceae. The eff ect of extract on the phagocytic activity of peripheral blood neutrophils and the main indicators of the humoral immune response (the number of antibody-forming cells of the spleen and the synthesis of anti-erythrocyte antibodies) were evaluated on a model of experimental immunodefi ciency in inbred mice. Extract from raw materials C. geoides showed a pronounced immunomodulatory eff ect, in some parameters superior to the eff ect of offi cinal tincture of Echinacea.

Russian Journal of Immunology. 2019;22(2-1):239-241
pages 239-241 views

CATALASE ACTIVITY OF CATALYTIC ANTIBODIES IN SCHIZOPHRENIA

Ermakov E.A., Smirnova L.P., Krotenko N.M., Semke A.V., Buneva V.N., Nevinsky G.A.

Abstract

In this work, the ability of catalytic antibodies (abzymes) in patients with schizophrenia and healthy donors to catalyze the decomposition of hydrogen peroxide was investigated. The polyclonal IgG in patients with schizophrenia and healthy donors was found to have catalase activity, and the activity of patient’s IgG was 1.7 times higher. The heterogeneity of abzymes with catalase activity by affi nity to metal ions, pH optimum, kinetic parameters is shown. A possible antioxidant function of catalytic antibodies with catalase activity is discussed.

Russian Journal of Immunology. 2019;22(2-1):242-244
pages 242-244 views

INFECTOLOGICAL PARAMETERS AS PREDICTORS OF FERTILITY FAILURE

Zherebyateva O.O., Mironov A.Y., Mikhailova E.A., Makhalova G.O., Kirgizova S.B., Fomina M.V., Lyashenko I.E.

Abstract

The state of factors of antimicrobial resistance in the secretions of the genital tract, and the immunoneutralizing ability of the urogenital microfl ora of healthy and infertile patients were investigated. Differences in the studied features are used as predictors of infertility.

Russian Journal of Immunology. 2019;22(2-1):245-247
pages 245-247 views

INFLUENCE TRIGGER INFECTIONS ON THE IMMUNE STATUS OF PATIENTS URINOGENOUS REACTIVE ARTHRITIS

Zhuravleva M.O., Meshcheryakov A.V.

Abstract

The influence of trigger infections on the immune status of 150 patients with UReA was studied. There was inhibition of T-dependent reactions, hyperproduction of Ig and CIC, inhibition of phagocytic activity of neutrophils, which can contribute to the persistence of infection in the body, chronic infl ammation and maintenance of autoimmune process. No signifi cant differences in immune parameters depending on the identifi ed infectious agent were found.

Russian Journal of Immunology. 2019;22(2-1):248-250
pages 248-250 views

THE CONDITION OF SYSTEMIC IMMUNITY IN WOMEN WITH EXTERNAL GENITAL ENDOMETRIOSIS AND HUMAN PAPILLOMA VIRUS

Zaynetdinova L.F., Koryaushkina A.V., Telesheva L.F.

Abstract

Genital endometriosis is an estrogen-dependent inflammatory disease, the etiology of which is not known. Given the high prevalence of HPV among women of reproductive age, as well as the development of endometriosis mainly in the same age group, we can assume the participation of HPV in the formation of features of systemic immunity in this disease. The results of the study showed the presence of HPV in 13 (18,8%) women with 1–2 stages of endometriosis and in 22 (24,4%) – with 3–4 stages of endometriosis. In the presence of HPV in women with 1–2 stages of endometriosis, it was established: an increase in the relative number of T-NK lymphocytes, a decrease in IL-2, IL-6 and an increase in serum IL-4, TNFα, IFNγ. With 3–4 stages – an increase in the absolute number of T-NK-lymphocytes, IL-4, IL-8, IL-10, TNFα, IFNγ in serum. Thus, in women with endometriosis in the presence of HPV, the features of systemic immunity have been identified, which may be associated with disease progression.

Russian Journal of Immunology. 2019;22(2-1):251-253
pages 251-253 views

THE DYNAMICS OF CHANGES IN THE IMMUNE STATUS OF THE MILITARY PERSONNEL IN TERMS OF PROFESSIONAL TASKS

Zaitseva N.S., Sizyakina L.P.

Abstract

The article presents the results of dynamic monitoring of soldiers who participated in special operations. The changes in the immune status were documented immediately upon the return of military personnel from a mission to areas with difficult operational conditions and the absence of their spontaneous recovery after 6 months.

Russian Journal of Immunology. 2019;22(2-1):254-256
pages 254-256 views

EFFECT OF PREGNANCY-SPECIFIC β1-GLYCOPROTEIN ON CHEMOKINE PROFILE OF CULTURED T-HELPER AND MONONUCLEAR CELLS

Zamorina S.A., Litvinova L.S., Yurova K.A., Khaziakhmatova O.G., Timganova V.P., Bochkova M.S., Khramtsov P.V., Rayev M.B.

Abstract

The effect of human pregnancy-specific β1-glycoprotein (PSG) on the production of chemokines by intact mononuclear cells and monocultures of CD4+ cells in vitro by Bio-Plex® multiplex immunoassay technology was studied. It has been established that PSG suppresses the production of IL-8, MIP-1α, MIP-1β, RANTES and MCP-1, chemokines, an elevated level of which is associated with spontaneous abortion. As a result, it can be concluded that PSG forms the fetoprotective chemokine profile of the studied immune cells.

Russian Journal of Immunology. 2019;22(2-1):257-259
pages 257-259 views

NEW ASPECTS OF IMMUNOPATHOGENESIS OF GRAVES’ DISEASE – CYTOKINES REGULATION AND CELLULAR INFILTRATION OF A THYROID GLAND AT AN AUTOIMMUNE THYROTOXICOSIS

Zdor V.V., Geltser B.I.

Abstract

It is revealed that significant changes in serum levels of a number of opposed cytokines with a significant increase of IFN-γ and IL-2 on the background of TGF-β1 deficiency initially and on the background of thiamazole therapy, which indicates Tregs dysfunction followed by a depletion phenomenon occur in Graves’ disease. Preservation of elevated IL-10 levels on the background of 6 months of thyrotoxicosis therapy indicates a negative role of the cytokine due to the well-known induction of Treg diff erentiation into Th17 cells with subsequent reduction in the number of Tregs. Along with the study of thyroid hormones, TSH and thyroid autoantibodies, it is advisable to monitor the serum levels of IL-2, IFN-γ, IL-6, sIL-6R, IL-17 and TGF-β1, initially and during the thiamazole therapy for adequate monitoring of the autoimmune process and prediction of a possible recurrence of the disease, including when deciding on the radical treatment. The study obtained convincing data on the participation of mastocytes in the pathogenesis of Graves’ disease, but not in the nodal euthyroid goiter. The active migration of mast cells to the thyroid gland in Graves’ disease, their degranulation in the intra- and interfollicular space and their expression of CD86 predetermines the ability of mast cells to participate in the antigen-presenting function in autoimmune thyrotoxicosis, which opens up prospects for personalized pathogenetic therapy of the disease.

Russian Journal of Immunology. 2019;22(2-1):260-263
pages 260-263 views

THE RELATIONSHIP OF POSTOPERATIVE COGNITIVE DYSFUNCTION WITH THE LEVEL OF METALLOPROTEINASE-2 AND INTERLEUKIN-17 AND TRANSFORMING GROWTH FACTOR β2

Zenina A.A., Bekerova D.F., I A., Markina L.D.

Abstract

30 patients after CABG were examined. The patients were divided into two groups on the basis of MoCa test results. Outcome monitoring of MMP-2, IL-17, TGFβ2 level in the blood serum was carried out 4 times by means of microparticle enzyme immunoassay before the surgery, after the surgery, as well as on the 1st and 7th day following the surgery. In patients with PCD the higher level of IL-17 was distinguished. Alongside with the low levels of GFβ2 and MMP-2 after the surgery, which is determined by a more vivid proinflammatory response and the insuffi ciency of the proinflammatory reaction.

Russian Journal of Immunology. 2019;22(2-1):264-266
pages 264-266 views

GLYCOPATHOLOGY OF FETOPLACENTAL-MATERNAL SYSTEM IN THE «GREAT OBSTETRICAL SYNDROMES» ASSOCIATED WITH DISORDERS OF DEEP PLACENTATION

Ziganshina M.M., Shilova N.V., Kulikova G.V., Khasbiullina N.R., Shchegolev A.I., Nokel A.Y., Dolgopolova E.L., Bovin N.V.

Abstract

The features of the glycophenotype of the placenta, the repertoire and the content of anti-glycan antibodies of the blood were studied using lectin histochemistry and glycochip in preeclampsia (PE) and fetal growth restriction (FGR), which indicate the development of glycopathology in the fetoplacentalmathernal system and the different pathogenesis of early and late PE and FGR.

Russian Journal of Immunology. 2019;22(2-1):267-269
pages 267-269 views

PROGNOSTIC SIGNIFICANCE OF CYTOKINES` TISSUE LEVELS IN SOME MALIGNANT TUMORS

Zlatnik E.Y., Bazaev A.L., Kochuev S.S.

Abstract

Levels of cytokines TNF-α, IL-1β, IL-2, IL-6, IL-8, IL-10, IFN-α, IFN-γ were evaluated in samples of tumor and peritumoral tissue of 36 patients with esophageal cancer and 56 patients with skin melanoma in comparison with their clinical course. By means of ROC-analysis we showed that cytokines` levels in tissues are of some use for prognosis of the risk of the tumors` early progression. High levels of IL-1β and IL-6 in tumor, IL-1β and IL-2 in peritumoral tissue characterize the melanoma`s microenvironment as the most dangerous in course of negative prognosis. As for the esophageal cancer it is related to high amounts of IL-1β, IL-8 and IL-10 in tumor, IL-8 and TNF-α in peritumoral tissue, TNF-α and IL-10 in the resection line.

Russian Journal of Immunology. 2019;22(2-1):270-271
pages 270-271 views

IMMUNOLOGICAL PREDICTORS OF SOCIAL FUNCTIONING IN CHILDREN WITH ENDOGENOUS PSYCHOSES

Zozulya S.A., Nikitina S.G., Simashkova N.V., Klyushnik T.P.

Abstract

The problem of the outcomes of the first psychotic episodes occurring in childhood as well as the level of disruption of social functioning (SF) is an actual problem of psychiatry. Taking into account the results of recent studies demonstrating the involvement of the immune system in the pathogenesis of endogenous mental disorders, the purpose of this work was to identify the immunological predictors of SF in children undergoing endogenous psychosis in accordance with the PSP scale. It is shown that the level of activation of the immune system (based on the “Neuro-immuno-test” immune indicators) at the psychotic stage can serve as a predictor of further social functioning SF of the child in the post-attack period.

Russian Journal of Immunology. 2019;22(2-1):273-275
pages 273-275 views

A METABOLIC SPECTRUM OF THE GUT MICROBIOTA FOR THE PATIENTS WITH ASTHMA

Zolnikova O.Y., Ivashkin V.T.

Abstract

A content and profiles of short chain fatty acids (SCFAs) were studied into the feces of the patients with bronchial asthma and healthy persons too. A decrease of the absolute content and profiles changing of the short chain fatty acids (SCFAs) were detected after the lactic acid fl ora metabolic activity decreasing of (bifidobacteria and lactobacilli). An increasing of these parameters (isoCn and isoCn / Сn) indicates on a destruction of the epithelial layer of the intestinal wall. The anaerobic index values have refl ected the redox potential of the intraluminal environment. As it was detected the anaerobic index changing could lead to the growth of the facultative aerobic and residual (conditionally pathogenic) aerobic microfl ora. The revealed SCFA’s changes give evidence about the pronounced disorders in the intestinal biotope’s microbiocenosis and this fact could be considered as one of the reasons of the bronchial asthma development.

Russian Journal of Immunology. 2019;22(2-1):276-277
pages 276-277 views

MOLECULAR CLONING OF HUMAN THYROID STIMULATING HORMONE RECEPTOR GENE

Zubkov A.V., Andreeva M.A., Sidorov A.V., Milovanova A.V., Butova L.G.

Abstract

Producing of recombinant TSHR fragments opens up new possibilities in the development of immunochemical tests to detect stimulating and blocking antibodies to TSHR, in studying the role of the antigenic structure of TSHR in the pathogenesis of autoimmune diseases of the thyroid gland. The original mRNA sequence of the α-subunit of the thyroid stimulating hormone receptor, not containing nucleotide substitutions, was cloned. The obtained recombinant vector pVAX1-TSHR, prepared for transfection into cells of mammals and for the expression of the recombinant protein of the α-subunit of the thyroid stimulating hormone receptor.

Russian Journal of Immunology. 2019;22(2-1):278-280
pages 278-280 views

IMMUNOPATOGENIC FEATURES OF HEMORRHAGIC FEVER WITH RENAL SYNDROME IN THE DYNAMICS OF THE INFECTIOUS PROCESS

Ivanov M.F., Zhestkov A.V., Suzdalcev A.A., Popova L.L., Balmasova I.P.

Abstract

Hemorrhagic fever with renal syndrome (HFRS) is an acute zoonotic viral infection, clinically manifested by a hemorrhagic syndrome and acute renal failure. The aim of the work was to study the dynamics of lymphocytes of innate and adaptive immune response in patients with HFRS during feverish, oliguric, polyuric periods and in reconvalescence. There were some features of the immunopathogenesis of HFRS: a high content in blood of regulatory T-lymphocytes, the prevalence in the initial stages of the disease reaction of natural killer cells, the delay of the development of adaptive cellular immune response involving cytotoxic T-lymphocytes.

Russian Journal of Immunology. 2019;22(2-1):281-283
pages 281-283 views

COMPLEX CORRECTION OF IMMUNE VIOLATIONS IN INTERNAL ENDOMETRIOSIS

Ivanova O.Y., Telegina O.V., Ponomareva N.A., Elagina K.A., Kolomoets I.I.

Abstract

42 patients with a verifi ed diagnosis of adenomyosis stage II‒III were under constant observation. The concentration of pro-, anti-infl ammatory and regulatory cytokines, components of the complement and their inhibitors was determined in the blood plasma and aspirate from the uterine cavity. Prior to treatment, an increase in systemic and local concentrations of pro-infl ammatory (TNFα, IL-1β, IL-6, IL-8, IL-17, IL-18), IFNγ, G-CSF, IL-2, anti-infl ammatory (IL-4, IL-1RA) cytokines, an increase in the level of the complement components (C3, C3a, C4, C5, C5a) with an imbalance in the content of their regulators (an increase in factor H and a decrease in the C1 inhibitor). The combination of standard treatment in combination with Ridostin, Hypoxen and Essentiale forte H eff ectively corrected altered immune status parameters. 

Russian Journal of Immunology. 2019;22(2-1):284-286
pages 284-286 views

THE ATOPIC ASTHMA DISEASE COMPLICATED BY THE SMALL INTESTINE BACTERIAL OVERGROWTH (SIBO)

Ivashkin V.T., Zolnikova O.Y.

Abstract

An efficacy of a gut microbiota control was investigated for the patients with atopic asthma. 45 patients with atopic asthma were included in the study. The results of our clinical and lab tests, PFTs, the lactulose hydrogen breath tests have been presented to evaluate SIBO. Under the standard SIBO’s therapy, the firrst group had being tested with Rifaximinum for the SIBO therapy during 7 days or Rifaximinum and with a succeeding probiotics therapy during 1 month. SIBO was diagnosed for 30 (67%) patients. We have detected the higher IgE level (p<0,01), the higher eosinophils level (р<0,001) in sputum and more significant decrease for FEV1 (р<0,01) in SIBO. The disorders correction of the intestinal microflora is accompanied the statistically significant decreasing of the immunological activity (p<0.01) and improvement of the external respiration function (p<0.001). So, SIBO is a significant factor aggravating the atopic asthma in patients.

Russian Journal of Immunology. 2019;22(2-1):287-289
pages 287-289 views

LOCAL PROTEIN LEVEL OF ACUTE PHASES IN WOMEN WITH CERVICAL INTRAEPITELIAL NEOPLASIA

Kalandarova A.N., Aripova T.U., Eshimbetova G.Z.

Abstract

The study of the level of proteins of the acute phase in cervical mucus in 48 women with cervical intraepithelial neoplasia (CIN). It was revealed that the level of C3 component of complement and lactoferrin was signifi cantly increased in women with CIN I and II degree. Moreover, a higher level of the studied proteins of the acute phase was observed in women with CIN against the background of PVI.

Russian Journal of Immunology. 2019;22(2-1):290-292
pages 290-292 views

INTERRELATION OF PROCALCITONIN AND CD64⁺ WITH BACTEREMIA AND SEVERITY OF ORGAN DYSFUNCTION

Kalashnikova A.A., Voroshilova T.M., Slashcheva O.M.

Abstract

To assess the risk of developing infectious complications and sepsis in critically ill patients, a number of laboratory markers are used, the main of which is procalcitonin, the less well known is the expression of CD64 on neutrophils. There is a close correlation between these indicators, however, in some cases, the results of tests for procalcitonin and CD64 contradict each other. The paper assesses the interrelation of procalcitonin and CD64 with bacteremia and severity of organ dysfunction. It was determined that the level of expression of CD64 is closely related to the presence of bacteria in the blood, whereas procalcitonin is not specifi c for bacteremia. A direct correlation was found between the severity of organ dysfunction and both indicators. This interrelation is more signifi cant for procalcitonin. The use of tests for procalcitonin and CD64 in the complex will improve the quality of examination of seriously ill patients.

Russian Journal of Immunology. 2019;22(2-1):293-295
pages 293-295 views

IMMUNE ASPECTS OF CANCER DEVELOPMENT

Karmakova T.A.

Abstract

Local and systemic immune responses are involved in the development of the malignant tumor at all stages of the process, from the appearance of transformed cellsto the clinical progression of the disease. The review includes the main provision of the modern concepts of oncogenesis, antitumor immune surveillance, immunoediting and the role of tumor-promoting chronic infl ammation.

Russian Journal of Immunology. 2019;22(2-1):296-298
pages 296-298 views

ANALYSIS OF PHAGOCYTIC ACTIVITY OF NEUTROPHILS DURING ATHEROSCLEROSIS

Kilinа O.Y., Dutova S.V., Saranchina Y.V., Matrosova T.A., Pol’shcha N.G., Kulakova T.S., Chanarin N.V., Shandakov P.I.

Abstract

The article presents the results of a comparative analysis of phagocytic activity of neutrophils, isolated from explants of atherosclerotic plaques obtained during endarterectomy in patients with atherosclerosis of the brachiocephalic and femoral arteries and peripheral blood neutrophils of the same patients. Phagocytic activity of neutrophils in atherosclerotic plaques was found to be statistically significantly reduced. We assume that the depletion of the functional ability of neutrophils in the composition of atherosclerotic plaques is associated with chronic inflammation in the intima of vessels in atherosclerosis.

Russian Journal of Immunology. 2019;22(2-1):299-301
pages 299-301 views

TUMOR NECROSIS FACTOR RECEPTORS IN PATIENTS WITH ACTIVE PULMONARY TUBERCULOSIS

Kireev F.D., Alshevskaya A.A., Laushkina Z.A., Sennikov S.V.

Abstract

Tumor necrosis factor (TNF) is a key cytokine in the immune response against Mycobacterium tuberculosis (M. tuberculosis). TNF can exhibit its biological eff ects in the presence of a suffi cient number of specifi c receptors on cells – types 1 and 2 membrane receptors (TNFR1 and TNFR2). The objective of this research was to study the diff erences in TNFR expression in patients with active pulmonary tuberculosis (aPTB) in comparison with the parameters of healthy individuals. Expression levels of TNFR1/2 on peripheral blood mononuclear cells (PBMCs) were analysed by fl ow cytometry. TNF and its types 1 and 2 soluble receptors (sTNFR1 and sTNFR2) levels were measured by enzyme-linked immunosorbent assay (ELISA). TNFR1 expression on PBMCs in aPTB patients was increased compared to healthy donors. sTNFR levels were lower in pulmonary tuberculosis patients. Data received suggest that TNF receptors play an important role in the immunopathogenesis of active pulmonary tuberculosis.

Russian Journal of Immunology. 2019;22(2-1):302-304
pages 302-304 views

FEATURES OF T-LYMPHOCYTES SUBPOPULATIONS IN CHRONIC HERPETIC INFECTION

Knysh S.V., Malkov V.A., Shiraeva A.V., Baibarina E.V.

Abstract

The one of the most signifi cant factor of immunosuppression is chronic herpetic infection. The absence of vaccine and unclear knowledge about reactivation`s pathogenesis also emphasize the importance of additional immune response study. We research the level of T-cell subpopulations during chronic herpetic infection. Treg percentage in main group was exceed one`s level in healthy control group. Also the signifi cant increasing of Th1\Th2 index was examined.

Russian Journal of Immunology. 2019;22(2-1):305-307
pages 305-307 views

MONO- AND COMBINED IMMUNOTHERAPY IMMUNOCOMPREPARED CHILDREN WITH RETURN CO-INFECTIONS

Kovaleva S.V., Chudilova G.A., Lomtatidze L.V., Nesterova I.V.

Abstract

In recent years, there has been an increase in co-infections with a variety of microbial associations, which makes it impossible to achieve positive eff ectiveness of standard therapeutic and preventive measures, increases the duration and severity of acute infectious processes, and chronic diseases, primarily the respiratory tract. The proposed programs of mono- and combination immunotherapy for immunocompromised children with recurrent co-infections demonstrate high clinical and immunological efficacy.

Russian Journal of Immunology. 2019;22(2-1):308-310
pages 308-310 views

DIAGNOSTIC VALUE OF IMMUNOLOGICAL MARKERS OF ALLERGIC BRONCHOPULMONARY ASPERGILOSIS IN PATIENTS WITH ASTHMA

Kozlova Y.I., Frolova E.V., Uchevatkina A.E., Filippova L.V., Vasileva N.V., Klimko N.N.

Abstract

Allergic bronchopulmonary aspergillosis (ABPA) is a severe lung disease caused by hypersensitivity to Aspergillus spp. The search for new diagnostic markers is essential for early detection of the disease in patients at risk and identifying eff ective treatment strategies. 21 patients with ABPA, 37 patients with asthma and 16 healthy subjects were examined. The content of thymic stromal lymphopoietin (TSLP), thymus-associated regulatory chemokine (TARC), IL-8, eosinophil count, total IgE levels and specifi c IgE to Aspergillus fumigatus were determined in serum by ELISA. The positive correlation of the TARC level with the indicators of total IgE, sIgE to Aspergillus fumigatus, the number of eosinophils and the negative correlation relationship with the FEV1 value confi rms the important diagnostic value of this pro-infl ammatory chemokine in patients with ABPA.

Russian Journal of Immunology. 2019;22(2-1):311-313
pages 311-313 views

SEARCH FOR PREDICTORS OF TARGET TREATMENT DISCONTINUATION DUE TO SIDE EFFECTS IN RHEUMATOID ARTHRITIS PATIENTS. DATA FROM MOSCOW ARTHRITIS REGISTRY

Koltsova E.N., Lukina G.V., Schmidt E.I., Zhilyaev E.V.

Abstract

The aim: to detect predictors of target drug withdrawal due to adverse events among patients with rheumatoid arthritis. Materials and methods: The study includes patients with rheumatoid arthritis from the Moscow Arthritis Registry (MAR), receiving treatment with biologics or tofacitinib. Results: Analysis includes 1230 treatment events in 696 patients. The mean age was 54,6±12,9 years. The mean observation time – 5.3 years. There were 146 cases of therapy discontinuation due to adverse events. Рresence of rheumatoid nodules, higher doses of glucocorticoids, lower doses of methotrexate were independent signifi cant predictors of increased risk of target treatment withdrawal due to side eff ects. Used target drug also showed independent signifi cant correlation with this risk. Conclusion: The use of full doses of methotrexate, reduce the use of glucocorticoids can be considered as a measure to prevent the development of adverse events in patients treated with target therapy.

Russian Journal of Immunology. 2019;22(2-1):314-316
pages 314-316 views

EVALUATION OF THE MENSTRUAL CYCLE VIOLATIONS OF ADOLESCENTS LIVING IN THE ORENBURG REGION

Komleva M.O., Komleva N.V., Smolyagin A.I.

Abstract

The paper presents the results of a survey of patients aged 15–17 years with menstrual irregularities (NMCs) living in the Orenburg regions with diff erent anthropogenic load. The greatest number of changes was found among the indicators of the relative content of CD3+, CD4+ and CD19+ lymphocytes, phagocytic index, IgM, estradiol, progesterone, testosterone levels, LH, 17-OH-progesterone and cortisol. The results indicate the signifi cance of the negative impact of anthropogenous loading on the gynecological morbidity of girls.

Russian Journal of Immunology. 2019;22(2-1):317-319
pages 317-319 views

VIRAL INFECTIONS FROM HERPESVIRIDAE FAMILY AND AUTOREACTIVITY IN BRONCHIAL ASTHMA

Konishcheva A.Y., Gervazieva V.B.

Abstract

The immune response to the viruses from Herpesviridae family was evaluated by determining the levels of virus-specifi c IgG-AT and IgE-AT to HSV 1, EBV, CMV using an adapted ELISA method in patients with bronchial asthma (BA), along with IgE- and IgG4-autoAbs a number of tissue Ags. We revealed that in patients with BA in a prevailed percentage of cases (88%) IgG-Abs for CMV, HSV and EBV were detected in high titers (1:800‒1:2000) as compared with healthy individuals (1:200‒1:400) with greater elevation in patients with severe BA. Direct associations between IgG-Abs for herpes viruses and IgE-autoAbs for collagen III and VI types, myosin, elastin and levels of IL-4 and total IgE directly confi rms the contribution of chronic forms of herpes infection to the maintenance of both IgE-mediated immune infl ammation and autoreactivity in patients with asthma.

Russian Journal of Immunology. 2019;22(2-1):320-322
pages 320-322 views

IMMUNE DISTURBANCES AT THE LOCAL LEVEL IN CHRONIC ENDOMETRITIS; IMMUNE REHABILITATION

Konoplya A.A., Gavrish S.A., Omasharifa Z.P.

Abstract

42 patients of reproductive age with a verifi ed diagnosis of chronic endometritis in the stage of incomplete remission were divided into 3 groups: the control group, receiving complex standard treatment and the main ones, where pharmacotherapy was supplemented with the administration of Gepon and Cytofl avin or a course of magneto-infrared laser therapy. Initially, an increase in the level of pro-infl ammatory and regulatory cytokines, activation of the complement system, an imbalance in the content of anti-infl ammatory cytokines, regulators of the complement system, a decrease in the sIgA level was established at the local level. The lack of eff ectiveness of the use of complex standard treatment in the correction of immune disorders was determined. The use of Gepon and Cytofl avin or magneto-infrared laser therapy eff ectively corrects disturbed parameters of the immune status.

Russian Journal of Immunology. 2019;22(2-1):323-325
pages 323-325 views

MODERN REQUIREMENTS TO THE EXPERT EVALUATION THE SPECIFIC SAFETY OF MEDICINAL PRODUCTS OF HUMAN IMMUNOGLOBULIN

Kornilova O.G., Husnatdinova E.S., Krivykh M.A., Olefir Y.V.

Abstract

Regulation of processes to ensure specific safety of human immunoglobulin drugs is designed to ensure the availability of eff ective and safe drugs on the Russian pharmaceutical market. Expertise within the framework of registration ensures the formation of conditions for the issuance of drugs corresponding to the level of safety. Compliance with the requirements for expert evaluation of the stages of pharmaceutical development, technological process, taking into account the control of critical stages of production, the finished form of the drug in terms of specific safety, allows to predict the degree of their negative impact on the hemostatic system, complement and kallikrein-kinin.

Russian Journal of Immunology. 2019;22(2-1):326-328
pages 326-328 views

CD4+ T-LYMPHOCYTE FUNCTION IS VIOLATED IN HIV-INFECTED PATIENTS WITH DISCORDANT RESPONSE TO ANTIRETROVIRAL THERAPY

Korolevskaya L.B., Saidakova E.V.

Abstract

In HIV-infected patients with a discordant response to antiretroviral therapy, CD4+ T-cell proliferative capacity, ability to synthesize IL-2, and resistance to apoptosis were accessed. It was shown that in those subjects, CD4+ T-cells are characterized by a decreased mitotic activity and IL-2 production; and an increased rate of programmed death. Disorders found could be the basis for the ineff ective CD4+ Tcell counts restoration in patients with a discordant response to the treatment.

Russian Journal of Immunology. 2019;22(2-1):329-331
pages 329-331 views

FLOW CYTOMETRIC ANALYSIS EXPRESSION MARKERS OF ACTIVATION ON CD4 T-LYMPHOCYTES AT RHEUMATOID ARTHRITIS

Kostolomova E.G., Suhovej U.G., Unger I.G., Akuneeva T.V., Krivonosova O.A., Zenkova T.V., Makeeva O.V., Shvets O.J.

Abstract

In research, it is studied coexpression CD69 and HLA-DR on sinovial T-lymphocytes at rheumatoid arthritis. The received results allow to assume, that in sinovial fluid there is a constant activation of T-cell that leads expression a marker CD69 on a surface chronically it stimulates lymphocytes only at the location the inflammation.

Russian Journal of Immunology. 2019;22(2-1):332-334
pages 332-334 views

АSSESSMENT OF THE EFFICACY OF IMMUNOMODULATORS IN EXPERIMENTAL PNEUMONIA

Kostyushko A.V., Yakovlev A.A., Abakumov A.I., Chagina E.A., Kharina A.S., Sotnikova D.D.

Abstract

Leukinferon and Roncoleukin aff ects cytokine status in pneumonia caused by Pseudomonas aeruginosa in the experiment.

Russian Journal of Immunology. 2019;22(2-1):335-337
pages 335-337 views

CHARACTERISTICS OF LOCAL CYTOKINE PROFILE IN PATIENTS WITH CHRONIC TONSILLITIS DEPENDING ON ETIOLOGY

Krasnitskaya A.S., Bocharova O.S., Pervov Y.Y., Klimkina T.N.

Abstract

Chronic tonsillitis today is one of the most common pathologies of ENT organs. In our study, the content of cytokine status in the wash water from the Palatine tonsils was analyzed in 164 patients aged 18 to 61 years with this diagnosis. Patients were divided into groups depending on the etiology of the disease. Based on the data obtained, we can more accurately conclude about the viral course of the process and evaluate the effectiveness of the therapy.

Russian Journal of Immunology. 2019;22(2-1):338-340
pages 338-340 views

THE IMMUNOLOGICAL INDICATORS OF PERSONALIZED PRESCRIPTIONS FOR ALLOIMMUNIZATION IN THE THERAPY OF IDIOPATHIC RECCURENT MISCARRIAGE

Krechetova L.V., Tetruashvili N.K., Vtorushina V.V., Nikolaeva M.A., Vanko L.V.

Abstract

This article represents the results of the lymphocyte’s subpopulation analysis of women with idiopathic reccurent pregnancy loss (RPL) after lymphocyte immune therapy (LIT). It reveals the dependence of successful childbearing in women with RPL on the cellular immune system, validates the term of alloimmune genesis of recurrent miscarriage is eligible. It identifi es the indicators for personalized prescriptions for LIT and indicators of favorable prognosis of successful pregnancy in woman with RPL after LIT.

Russian Journal of Immunology. 2019;22(2-1):341-343
pages 341-343 views

THE ANALYSIS OF PERIPHERAL BLOOD CYTOKINES IN WOMEN WITH RECURRENT SPONTANEOUS ABORTION

Kroshkina N.V., Kust A.V., Kozelkova E.V.

Abstract

The level of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, IFNγ, TNFα, GM-CSF in the peripheral blood of women with recurrent spontaneous abortion and uncomplicated pregnancy was investigated at 5–12 weeks of gestation. It was established, that the serum level of IL-1β, IL-22, IL-23 and IL-27 in women with habitual abortion increased, and the tendency to the elevation of serum level of IL-10 и IL-13 was seen.

Russian Journal of Immunology. 2019;22(2-1):344-346
pages 344-346 views

DYNAMICS OF CYTOKINE PRODUCTION OF BLOOD SERUM AND AMNIOTIC FLUID AT A THREAT OF PREGNANCY INTERRUPTION

Krukier I.I., Erdzhanyan L.L., Avrutskaya V.V., Rozhkov A.V., Nikashina A.A., Petrov Y.A.

Abstract

The level of cytokines (TNF-α, TGF-β, IL-1β and IL-10) in serum and amniotic fluid medium be representative of the state of interleukin balance in gestational tissues, and its definition has a high informative value for judging the course of pregnancy and fetal development fetus.

Russian Journal of Immunology. 2019;22(2-1):347-349
pages 347-349 views

THE ROLE OF NEUTROFILS IN THE PATHOGENESIS OF PREECLAMPSIA

Kudryashova A.V., Panova I.A., Rokotyanskaya E.A.

Abstract

The aim of the work was to assess the nature of changes in the functional activity of neutrophils in women with preeclampsia and to identify their significance in the development of hypertension and proteinuria. For neutrophils of women with preeclampsia, the following were observed: increased levels of CD11b+, CD31+, CD49b, TLR4+, AnnexinV+PI+, IFNγ+, Granzyme B+, NBT-active cells, serum G-CSF, IL-8, IFNγ and Fractalkine; high level of MMP-9 synthesis; decreased levels of CD95+, CX3CR1+, CD62L+ and CD99+ cells. Correlation was noted between: the level of CD62L+, CD99+, CXCR1+ neutrophils, serum IL-8 and impaired elastic properties of blood vessels; CX3CR1+ neutrophil level and mean arterial pressure and proteinuria in a single portion of urine.

Russian Journal of Immunology. 2019;22(2-1):350-352
pages 350-352 views

SERUM CONTENT OF CYTOKINES REGULATING PROLIFERATION AND DIFFERENTION OF LYMPHOCYTES IN HYPERTENSIVE DISORDERS IN PREGNANT WOMEN

Kudryashova A.V., Panova I.A., Rokotyanskaya E.A., Panaschatenko A.S.

Abstract

Relative and absolute lymphocyte amounts in the clinical (or common) blood analysis, serum levels of IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-15, IL-17А were investigated in pregnant women with chronic arterial hypertension, preeclampsia, and with preeclampsia on the background of chronic arterial hypertension as well as uncomplicated pregnancy (control). In all groups of women with hypertensive disorders there was a decrease in the level of lymphocytes and IL-2 and IL-15 in the blood. In contrast to the control, preeclampsia increased the contents of IL-5 and IL-6. Besides, severe preeclampsia increased the level of IL-4, IL-9, IL-17A, with chronic arterial hypertension IL-12p70. Thus, in hypertensive disorders in pregnant women, the production of cytokines regulating the proliferation of lymphocytes decreases. However, in preeclampsia, the contents of cytokines, which determine the maturation and diff erentiation of eff ector cells, increases.

Russian Journal of Immunology. 2019;22(2-1):353-355
pages 353-355 views

THE STERIC AVAILABITY OF THYROID PEROXIDASE EPITOPES IN ENZYME IMMUNOASSAY FOR THE DETERMINATION OF AUTOANTIBODIES

Kuzmina N.S., Zubkov A.V., Sviridov V.V.

Abstract

Evaluation of circulating autoantibodies (auto-At) to thyroid peroxidase (TPO) is widely used in the clinic to identify / confirm autoimmune diseases of the thyroid gland. Despite significant progress in creating tests for auto-At detection, the issues of their validation, standardization of methods attract close attention of both developers and users. Comparative studies of the level of antibodies in the blood serum of patients with diff use toxic goiter, autoimmune thyroiditis using diagnosticumof diff erent manufacturers showed that the discrepancies are associated with the TPO epitope structure immobilized on the solid phase. It is proposed to use monoclonal antibodies to control the antigenic structure of TPO as the process of isolation of the native protein, and the production of tests for the analysis of At to TPO.

Russian Journal of Immunology. 2019;22(2-1):356-358
pages 356-358 views

GLUTAMAT-MEDIATED MECHANISMS OF REGULATION OF VLA-4 EXPRESSION IN MULTIPLE SCLEROSIS

Kuzmina U.S., Bakhtiyarova K.Z., Vakhitova Y.V.

Abstract

The effect of blockade of AMPA and NMDA glutamate receptors on the surface expression of integrin VLA-4 in intact and antigen-activated CD69+CD25, CD69CD25+ and CD69+CD25+ subpopulations of T-lymphocytes obtained from healthy individuals and patients with multiple sclerosis (MS) was studied. The assessment of the level of surface expression of integrin was performed using flow cytometry using immunofluorescent staining. According to obtained data, blockade of NMDA receptors is not accompanied by a change in the surface expression of VLA-4 in all experimental groups. At the same time, differences in the consequences of the blockade of AMPA receptors in patients with MS compared with the group of healthy individuals were revealed: against the background of NBQX in healthy individuals, an increase in surface expression of VLA-4 was observed on cells with the CD69CD25+ phenotype, while in patients with MS a decrease in the studied parameter was found in CD69+CD25 cells. Thus, glutamate receptors are involved in the regulation of the expression of VLA-4 localized on T-lymphocytes, which reveals the possible mechanisms by which the immunomodulating functions of glutamate are realized in MS. 

Russian Journal of Immunology. 2019;22(2-1):359-361
pages 359-361 views

INFECTIOUS STATUS IN WOMEN WITH THREATENED PREGNANCY LOSS AND HABITUAL MISCARRIAGE IN ANAMNESIS

Kust A.V., Kozelkova E.V., Sadygova A.V., Borzova N.Y., Smirnova A.V., Batrak N.V.

Abstract

The aim of the work was to define the type of the infectious status in women with early threatened abortion. It was established, that early threatened abortion is associated with the presence in the peripheral blood of markers of acuteviral and bacterial infection. Mixt-infection makes an important contribution to the genesis of early threatened abortion in recurrent spontaneous abortion.
Russian Journal of Immunology. 2019;22(2-1):362-364
pages 362-364 views

THE VALUE OF INFLAMMATORY MEDIATORS IN THE PATHOGENESIS OF PRETERM BIRTH

Levkovich M.A., Krukier I.I., Erganian L.L., Kalinina A.V.

Abstract

The study of the levels of proinflammatory cytokines and neopterin in pregnant women with preterm labor against the background of placental insufficiency (PN). Preterm labor has been found to be associated with an increase in serum and amniotic fluid in IL-6, IL-8 and neopterin levels. Identified biological markers can be used to predict preterm labor in patients with placental insuffi ciency for the purpose of early administration of adequate therapy.

Russian Journal of Immunology. 2019;22(2-1):365-367
pages 365-367 views

CYTOKINE PROFILE OF NEWBORN CHILDREN WITH GENERALIZED HERPETIC INFECTION

Levkovich M.A., Kravchenko L.V., Levkovich A.Y., Pyatikova M.V.

Abstract

New data confirming the role of immune system disorders in the pathogenesis of generalized herpes infection are presented. It has been established that in newborns the development of generalized herpes virus infection is associated with impaired cytokine balance, with a tendency to T2 directional type of immune response, insufficiency of IFNα and IFNγ production.

Russian Journal of Immunology. 2019;22(2-1):368-370
pages 368-370 views

CORRECTION OF IMMUNE DISORDERS IN EXPERIMENTAL ACUTE LIVER ISCHEMIA

Litvinova E.S., Konoplya A.I.

Abstract

In experimental acute ischemia of the liver, suppression of the formation of the cellular and humoral forms of the immune response to sheep erythrocytes, a decrease in the functional and metabolic activity of circulating blood neutrophils has been established. It was established that proteins of the culture fluid of allogeneic hepatocytes of newborn rats with MM less than 130 kD have a pronounced immunocorrective efficiency, and proteins more than 130 kD do not possess such activity.

Russian Journal of Immunology. 2019;22(2-1):371-373
pages 371-373 views

IMMUNOENDOCRINE DISTURBANCES IN PATIENTS WITH SCHIZOPHRENIA IN THE PROCESS OF ANTIPSYCHOTIC THERAPY

Lobacheva O.A., Vetlugina T.P., Kornetova E.G., Semke A.V.

Abstract

Study of dynamics of immunoendocrine parameters in 27 patients with schizophrenia has shown that acute period of schizophrenia after long-term maintenance treatment with quetiapine is accompanied by immune-hormonal imbalance characterized by cellular immune deficit, increase in the concentration thyroid-stimulating hormone, reduction in thyroid hormones and prolonged elevation of the level of cortisol in blood. The obtained data will allow further revealing the criteria of personalized approach to treatment of patients with possible joining additional pharmacotherapy from the first days of the admission of patients in the hospital.

Russian Journal of Immunology. 2019;22(2-1):374-376
pages 374-376 views

DETERMINATION OF THE FREQUENCIES OF IL17A AND IL17F GENE POLYMORPHISMS IN WOMEN WITH MISCARRIAGE

Loginova Y.V., Abramovskikh O.S., Nikonova T.I.

Abstract

The paper presents the results of the analysis of the frequency distribution of alleles and genotypes of polymorphic sites rs2275913 of gene IL17A and rs763780 of gene IL17F in women with miscarriage. Statistically signifi cant differences in the distribution of alleles and genotypes of oligonucleotide polymorphisms rs2275913 of gene IL17A and of gene IL17F in women with miscarriage in comparison with a group of conditionally healthy women with two or more successful pregnancies paired with the same partner have not been identified.

Russian Journal of Immunology. 2019;22(2-1):377-379
pages 377-379 views

IMMUNOREHABILITATION DRUGS OF MAGNESIUM IN ACUTE DESTRUCTIVE PANCREATITIS IN THE EXPERIMENT

Loktionova A.V., Bystrova N.A., Loktionov A.L.

Abstract

Studies were conducted on 108 healthy Wistar rats weighing 150‒200 g. Acute destructive pancreatitis (ADP) was modeled on the eve of the removal of animals from the experiment ligation of the ducts of the pancreas with subsequent stimulation by neostigmine. Investigated the influence of preparations of magnesium (Magne B6, Magnelis and Panangin) in the context of ADP on immunological reactivity. In ADP established reduction of phagocytosis, increased oxygen-dependent metabolism of circulating blood granulocytes, suppression of the formation of humoral and cellular forms of immune response. The most eff ective in correcting the parameters of innate and adoptive immunity was Magne B6, then, according to the degree of decrease in the effectiveness of Magnelis and Panangin.

Russian Journal of Immunology. 2019;22(2-1):380-382
pages 380-382 views

IMMUNOLOGICAL PHENOTYPES OF TIMOMEGALY IN INFANTS WITH ACUTE BRONCHITIS

Lyubimov D.S.

Abstract

Of the 104 hospitalized babies with acute bronchitis, an x-ray examination showed thymomegaly in 45 (46.8%). Against the background of depression of cellular immunity and hypocorticism, three immunological phenotypes of thymomegaly were revealed. Immunocorrection with taktivin and thymalin was carried out differentially, taking into account the clinical and immunological features of each phenotype.

Russian Journal of Immunology. 2019;22(2-1):383-384
pages 383-384 views

EFFICIENCY OF THE APPLICATION OF IMMUNE CORRECTIVE THERAPY IN OUTSIDE GENITAL ENDOMETRIOSIS

Magzumova N.M., Musakhodzhaeva D.A., Ismoilova D.U.

Abstract

38 women with external genital endometriosis (NGE) of II‒III degree were examined, which consisted of 2 groups: 1st group ‒ 20 patients in whom Roncoleukin was injected into the peritoneal cavity during laparoscopy, and after surgery for 2nd, 4th Every day and then ‒ 2 times a week for 3 weeks was administered intravenously at a dosage of 0.5 mg. The second group consisted of 18 women with NGE, in whom, after laparoscopy, buserelin depot was administered as an anti-relapse therapy with 3.75 mg intramuscularly once a month for 6 months. Cytokine levels (IL-1β, IL-2, IL-6, IL-8, TNFα and IFNγ) were studied in the peritoneal fl uid in women with HEG before and after treatment. An imbalance of the studied cytokines was detected in patients, which was restored after the application of immunocorrection.

Russian Journal of Immunology. 2019;22(2-1):385-387
pages 385-387 views

sIgE TO BACTERIAL ALLERGENES IN PATIENTS WITH CHRONIC INFLAMMATORY PULMONARY DISEASES

Mazurina S.A., Konishcheva A.Y., Ospelnikova T.P., Gervazieva V.B.

Abstract

Infections colonization colonization of the bronchopulmonary biotope is considered as one of the likely factors for maintaining the inflammatory process with a predominance of the Th2 immune response in patients with chronic diseases of the lung airway. The aim of the study was to assess the IgE immune response to the main microorganisms lung airway. Patients and methods: In the blood serum of 69 patients with BA, COPD and АСОS, the content of total IgE and sIgE for bacterial allergens, sIgA level in saliva was determined by ELISA. Results. In patients with АСОS, a wider spectrum of sIgE to bacterial allergens is presented, against the background of reduced levels of sIgA in saliva, in comparison with patients with asthma and COPD. This may indicate the involvement of innate immunity due to the IgE system in relation to anti-infective protection in patients with АСОS.

Russian Journal of Immunology. 2019;22(2-1):388-390
pages 388-390 views

THE EXPRESIION OF NCR RECEPTORS BY ENDOMETRIAL NATURAL KILLERS IN WOMEN WITH UTERINE LEIOMYOMA

Malyshkina D.A., Antsiferova Y.S., Dolgushina N.V.

Abstract

The patients with uterine leiomyoma demonstrate the signifi cant elevation of NKp44 and NKp46 expression by endometrial CD56+ lymphocytes. The high proliferation of leiomyoma is not associated with changes of NCR expression by endometrial NK. In the case of low proliferation the level of NK, CD56+NKp46+ and CD56+NKp46+NKp44+ lymphocytes is increased in comparison to the control group and group of patients with high expression of Ki67 in leiomyoma tissue.

Russian Journal of Immunology. 2019;22(2-1):391-393
pages 391-393 views

IMMUNOBIOCHEMICAL PREDICTORS FOR COMPLICATIONS IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION AFTER CORONARY STENTING

Markelova E.V., Grachev N.I., Semenikhin A.A., Karaulov A.V.

Abstract

The reason of study was assessing the prognostic ability of immunobiochemical parameters in patients with myocardial infarction after coronary arteries stenting. Blood sampling was performed before the operation, on the second and seventh days to determine IL-17, IFNγ and MMP-9. The predictive ability of IL-17 before intervention (10.21‒19.47 pg/ml), IFNγ (10.54‒51.27 pg/ml) and MMP-9 (426.80‒978.38 ng/ml) on the second day after surgery. These indicators increase the risk of complications in the early postoperative period.

Russian Journal of Immunology. 2019;22(2-1):394-396
pages 394-396 views

CAFFEINE-MODULATED IMMUNE CELLS EFFECT ON BEHAVIOR PATTERNS IN DEPRESSIVE-LIKE ANIMALS

Markova E.V., Knyazheva M.A., Amstislavskaya T.G.

Abstract

Disruption of neuroimmune interaction is an essential link in the pathogenesis of behavioral disorders.The unidirectional influence of most psychoactive substances on nervous and immune systems makes it possible to consider immune cells as model objects for influencing the intersystem functional relationship.The purpose of this work was to study the eff ect of the caff eine ‒ modulated immune cells transplantation on the behavioral patterns in depressive-like animals. The results show a corrective depressive-like behavior eff ect, resulting in a decrease in anhedonia, stimulation of behavior in the open field and a significant increase in the time periods of mobility in the forced swimming test registered against the background of changes in the braincytokines content.

Russian Journal of Immunology. 2019;22(2-1):397-399
pages 397-399 views

STEP-BY-STEP SIMULATION RISK REDUCTION OF PRIVATE ACUTE RESPIRATORY VIRAL INFECTION CASES IN PATIENTS WITH DIFFERENT IMMUNE STATUS

Martynov A.I., Fedoskova T.G., Feofanova T.V., Zelenova Z.V.

Abstract

A step-by-step statistical model for calculating the risks of frequent annual cases of acute respiratory viral infection in adults in the conditions of changing the magnitude of the harmful eff ects is proposed. The algorithm was tested on the data of two groups of workers of the chemical enterprise, which diff ered in size of the corresponding immune markers. It is shown that with the same reduction in the level of harmful eff ects, the rate of risk reduction in the groups diff ered by 2.5 times. Thus, the model can be used to select a priority group of employees in the conditions of a given impact on the production factor in order to minimize the value of the specifi ed risk for them.

Russian Journal of Immunology. 2019;22(2-1):400-402
pages 400-402 views

STUDY OF ANTIBODIES TO β2-GLYKOPROTEIN-I AND DOMAIN I IN WOMEN WITH RECURRENT PREGNANCY LOSS

Menzhinskaya I.V., Van’ko L.V.

Abstract

Antibodies to β2-GP-I are an independent risk factor for recurrent pregnancy loss (RPL).A good agreement between the results of ELISA and chemiluminescent immunoassay was shown in the determination of medium and high anti-β2-GP-I antibody concentrations. Antibodies to domain I were often found in the patients seropositive for anti-β2-GP-I antibodies. Detection of antibodies to domain I has important clinical significance for the stratification of patients by risk of thrombosis and obstetric complications.

Russian Journal of Immunology. 2019;22(2-1):403-405
pages 403-405 views

THE ROLE OF TLR9 AND COMPONENTS OF THE INFLAMMASOMA COMPLEX IN IMMUNOPATHOGENESIS OF PSORIASIS

Merkushova E.D., Khasanova E.M., Switich O.A., Batkaeva N.V., Gitinova M.M., Gankovskaya L.V.

Abstract

Psoriasis is an autoimmune disease in pathogenesis of which are involved autoinfl ammatory reaction too. An important role in the development of psoriatic infl ammation is given to the activation of infl ammasome. In this study, we investigate the expression of TLR9 genes, components of the infl ammasome complex (NLRP1, NLRP3), and eff ector molecules IL-1β and IL-18 in healthy and aff ected skin bioptates in 35 patients with severe psoriasis was studied using PCR-RT. In the psoriatic focus was found increasing the level expression of TLR9 genes 4.1 fold, NLRP1–6 fold. Analysis of interleukin gene expression revealed a 10-fold increase in IL-18 gene expression in the psoriatic focus, while IL-1β gene expression was comparable to its expression in healthy skin bioptates.

Russian Journal of Immunology. 2019;22(2-1):406-408
pages 406-408 views

IL-10 PRODUCTION BY PLACENTAL MACROPHAGES IN DEPENDENCE TO IL-10 POLYMORPHISM

Mileyeva P.L., Voronin D.N., Boyko Y.L., Fetisova I.N.

Abstract

IL-10 polymorpism А-1082G but not А-592C is the significant factor in the regulationof immune ractions at the placental level and it can be used as one of the predictors of the intrauterine growth retardation development.

Russian Journal of Immunology. 2019;22(2-1):409-410
pages 409-410 views

ESTIMATION OF PHYSIOLOGICAL AND MORPHOLOGICAL PARAMETERS IN RATS BORN FROM PASSIVALLY SMOKED FEMALES. MESSAGE2

Mikhailova I.V., Stadnikov A.A., Pushkareva L.A., Isengulova A.A., Kuzmicheva N.A., Shirshov O.V., Tikhonov V.V., Miroshnichenko I.V.

Abstract

It was established that the administration of the immunomodulator KK1 to pregnant rats subjected to passive smoking resulted in a positive dynamic of somatic development and morphological changes in the lymphoid organs.

Russian Journal of Immunology. 2019;22(2-1):411-413
pages 411-413 views

TRACE ELEMENT DISORDERS IN CHILDREN WITH ATOPIC DERMATITIS AND CONCOMITANT FORMS OF ALLERGY

Molokova A.V.

Abstract

All children with atopic dermatitis were found to have polymicroelement disorders – deficiency of essential (in 100% of cases), most often selenium, calcium, manganese, iron, copper, zinc; and accumulation of toxic trace elements in 83.6% – mainly molybdenum, bismuth, rubidium, lead, bromine, strontium. In patients with a combination of atopic dermatitis and bronchial asthma was dominated by politicalamity disorders – lack of selenium, calcium, copper, Nickel, molybdenum, iron, zinc and accumulation of chromium, lead, Nickel, rubidium, and bromine. In the groups of patients with isolated course of atopic dermatitis, with a combination of atopic dermatitis and bronchial asthma, it is possible to distinguish the same type of changes in the composition of trace elements: in both groups there was a deficiency of selenium, zinc, and the accumulation of lead.

Russian Journal of Immunology. 2019;22(2-1):414-416
pages 414-416 views

POLYMORPHISM OF ENZYMES OF BIOTRANSFORMATION OF XENOBIOTICS (GLUTATHIONE S-TRANSFERASES M1, T1, P1) IN PATIENTS WITH A COMBINATION OF ATOPIC DERMATITIS AND BRONCHIAL ASTHMA

Molokova A.V.

Abstract

The study of polymorphism of glutathione S-transferases M1, T1, P1 in children with allergic diseases showed a predominance of mutant enzyme genotypes in severe atopic dermatitis and asthma. Genotypes GSTM1«-», GSTT1«-» are signifi cant for the early formation of atopic dermatitis and bronchial asthma. Predisposition to combined atopy was registered for haplotypes M1«+»/T1«-»/P1ile105/Ile105 (OR=4.92; 95% CI 0.67–36.53) (р<0,05).

Russian Journal of Immunology. 2019;22(2-1):417-419
pages 417-419 views

CARACTERIZATION OF DISTRIBUTION OF MYELOID-DERIVED SUPPRESSOR CELLS AND MONOCYTES IN DIFFERENT CLINICAL VARIANTS OF ANKYLOSING SPONDYLITIS

Morenkova A.Y., Tikchonova M.A., Sizikov A.E., Chumasova O.A., Sulutyan A.E., Chernykh E.R.

Abstract

The frequencies of myeloid-derived suppressor cell (MDSC) and monocyte subpopulations in patients with ankylosing spondylitis (AS) have been investigated. AS patients, especially with axial disease, had decreased proportion of granulocytic MDSC. In contrast, the frequencies of monocytic MDSC were signifi cantly increased. The proportion of non-classical monocytes was increased in AS patients without peripheral manifestations. Of note, non-classical monocyte levels positively correlated with disease activity.

Russian Journal of Immunology. 2019;22(2-1):420-422
pages 420-422 views

REGULATORY T-CELLS IN ACUTE ISCHEMIC STROKE AND THEIR RELATION WITH INFLAMMATORY RESPONSE AND DEVELOPMENT OF INFECTIOUS COMPLICATIONS

Morozov S.A., Pronkina N.V., Starostina N.M., Leplina O.Y., Ostanin A.A., Shtobbe A.A., Chernykh E.R.

Abstract

The study has demonstrated a signifi cant increase in percentage of circulating CD4+CD25hi regulatory T cells and a decrease in Th1 (CD4+IFNγ+ N cells), as well as elevated leukocyte count, neutrophil-tolymphocyte ratio and CRP level during the first 24–48 hours after onset of ischemic stroke (IS). O note, patients with poststroke infection had less pronounced increase in CD4+CD25hi T-cells and the decrease in CD4+IFNγ+ T lymphocytes, while inflammatory response was significantly higher than in the opposite group. The largest difference between groups was observed when assessing the ratio of CD4+IFNγ+ and CD4+CD25hi T cells. The ratio of CD4+IFNγ+/ CD4+CD25hi in patients with infections was 3 times higher than in the group without infectious complications, correlated with the concentration of CRP (R = 0.54; p = 0.02) and the value of ratio > 1.14 allowed to predict infection with a specificity of 85.7% and a sensitivity of 68.8%. 

Russian Journal of Immunology. 2019;22(2-1):423-425
pages 423-425 views

PROGNOSTIC VALUE OF INTRACELLULAR ADHESION MOLECULES IN HEMOLYTIC-UREMIC SYNDROME

Moskalets O.V., Pankratenko T.E.

Abstract

The article presents the results of a study assessing the diagnostic and prognostic significance of determining the content of soluble intercellular adhesion molecules in children with typical and atypical hemolytic-uremic syndrome.

Russian Journal of Immunology. 2019;22(2-1):426-428
pages 426-428 views

CHARACTERISTICS OF INFLAMMATORY RESPONSE IN PATIENTS AFTER INTRAOCULAR LENS SUGERY

Moskalets O.V.

Abstract

Aim. To study prognostic value of some laboratory markers (anti-DNA antibodies, cell adhesive molecules, neopterin) in patients with exudative inflammation after inraocular lens surgery.

Materials and methods: 21 in-patients with postoperative iridicyclitis and endophthalmitis were included. The assays were taken twice: after admission and before discharging. The follow-up period was 6 months.

Results. Preliminary data show that high serum levels of sVCAM, sICAM and anti-DNA antibodies, as well as very low levels of anti-DNA antibodies seems to be associated with poor outcomes in those patients (enucleation, blindness, lens extraction).

Conclusion. Small cohort doesn’t allow make strict conclusion about prognostic value of these laboratory markers. The study should be continued. 

Russian Journal of Immunology. 2019;22(2-1):429-431
pages 429-431 views

THE VASCULAR ENDOTHElial GROWTH FACTOR IN THE TREATMENT OF CHRONIC INFLAMMATORY PERIODONTAL DISEASES

Mudrov V.P., Demyashkin G.A., Nelyubin V.N., Fomenkov I.S., Lysjuk E.Y., Vorobieva E.S., Ivanov S.Y.

Abstract

Periodontal disease is associated with progressive loss of bone and soft periodontal tissue. The use of biodegradable hydrogel with vascular endothelial growth factor (VEGF) contributing to the settlement of scaff old by fi broblasts with the subsequent formation of Mature connective tissue of the periodontal ligament is promising. Examined and treated 10 patients in the Department of maxillofacial surgery UKH № 4. Comprehensive treatment included standard drug therapy and based on carboxymethylcellulose and VEGF. As a result of treatment, the concentration of pathogenic microflora of periodontium was signifi cantly reduced. The concentration of VEGF in the periodontal pocket decreases after the treatment and normal histoarchitectonics of the gingival mucosa was found. Immunohistochemical methods showed a decrease in extracellular secretion of VEGF, as well as the construction of fi bers not only from “young” collagen type III, but also collagen type I. This indicates a decrease in the activity of inflammation and activation of the plastic processes of the fi brous component, leading to the strengthening of the periodontal structures on the background of the treatment. Thus, not only stimulation of regeneration was noted, but also a pronounced anti-inflammatory eff ect of hydrogel with a vascular endothelial growth factor having the potential to stimulate regeneration of periodontal tissues.

Russian Journal of Immunology. 2019;22(2-1):432-434
pages 432-434 views

CYTOKINE DYNAMICS AS A RESULT OF PHYTO-FLAVANOID EXPOSURE IN WOMEN WITH UTERINE MYOMA

Narzullaeva N.S., Musakhodzhaeva D.A., Abdurakhmanov M.M., Ikhtiyarova G.A.

Abstract

A study was conducted to study the effectiveness of the use of the herbal preparation Bioflazid in 28 women with a simple form of uterine fibroids. The levels of pro- (IL-6, IL-8, IL-18, and TNFα) and anti-inflammatory (IL-10) cytokines in serum were studied. Positive dynamics of both clinical parameters and levels of studied cytokines was revealed.

Russian Journal of Immunology. 2019;22(2-1):435-437
pages 435-437 views

DIFFERENTIATED APPROACHES TO TERGETED INTERFERON AND IMMUNOTHERAPY OF ATYPICAL CHRONIC ACTIVE INFECTION CAUSED BY EPSTEIN-BARR VIRUS

Nesterova I.V., Khalturina E.O., Stepanov O.G., Malinovskaya V.V.

Abstract

The problem of effective therapy of atypical chronic active EBV infection associated with chronic fatigue syndrome (AHA-VEBI) is extremely relevant at the present time. The study revealed the features of the defective functioning of the system of antiviral immune protection, as well as violations in the interferon system in patients suff ering from AHA-WEBI. The main clinical syndromes associated with AHA-WEBI were determined, immunopathogenetic features were clarified, the disease arose and progressed against the background, algorithms of complex diagnostics, including immunodiagnosis, and therapy were developed. The concept of diff erentiated, complex, etio- and immunopathogenetic therapy was developed and tested.

Russian Journal of Immunology. 2019;22(2-1):438-441
pages 438-441 views

DYSFUNCTION OF THE IMMUNE SYSTEM VALUES IN CHRONIC CERVICITIS GONOCOCCAL ETIOLOGY

Nefed’eva J.V., Ziganshin O.R.

Abstract

In work character immune disturbances at system and local levels at sick of women with gonococcal infection.

Russian Journal of Immunology. 2019;22(2-1):442-444
pages 442-444 views

IMMUNOENDOCRINE AND PSYCHOLOGICAL CRITERIA OF PREDICTING THE EFFICIENCY OF THERAPY IN PATIENTS SUFFERING FROM ADJUSTMENT DISORDER WITH ANXIETY-DEPRESSIVE SYMPTOMS

Nikitina V.B., Lobacheva O.A., Belokrylova M.F., Rudnitsky V.A., Epanchintseva E.M.

Abstract

According to the results of psychological and immunoendocrine examination of patients with adjustment disorder prior to treatment, it is possible to predict the efficiency of standard psychopharmacotherapy. A high level of state, trait anxiety and prolactin concentration, low values of the number of natural killer cells and the phagocytic index of neutrophils can be considered as predictors for an ineffective treatment of adjustment disorders.

Russian Journal of Immunology. 2019;22(2-1):445-447
pages 445-447 views

INTERFERON SYSTEM IN PATIENTS WITH CHRONIC DISEASES DURING IMMUNOMODULATING THERAPY

Оspelnikova T.P., Мorozova O.V., Mikhailovа N.A., Ershov F.I.

Abstract

The analysis of interferon (IFN) I (α, β), II (γ) and III (λ) types by RT-PCR-RV, ELISA, the determination of the biological activity of IFN in the dynamics of the treatment of chronic immuno-mediated diseases with immunomodulating drugs. Different degrees of severity, reduced biological activity of IFN type I and II in chronic urogenital infections and bronchial asthma (BA) was corrected by using IFN inducers in basic therapy. In patients with asthma, an imbalance of RNA of IFN types I and III was detected. The inclusion of cycloferon reduced by 2.5 times the frequency of BA exacerbations during the year. As a result of treatment of patients with multiple sclerosis (MS) with recombinant IFNβ-1a for more than 12 months.there was a decrease in increased expression of IFN I genes and IFN II type proteins, stabilization of the neurological state. Thus, in chronic infections and BA, the deficiency and imbalance of IFN is compensated by the introduction of IFN inducers. In contrast, a decrease in elevated levels of IFN RNA in MS is achieved with prolonged administration of the recombinant drug IFNβ-1a, acting, possibly, on the basis of reverse regulation.

Russian Journal of Immunology. 2019;22(2-1):448-450
pages 448-450 views

AUTOANTIBODIES TO THE MYELIN BASIC PROTEIN IN PATIENTS WITH SCHIZOPHRENIA HAVE PROTEOLYTIC ACTIVITY

Parshukova D.A., Buneva V.N., Kornetova E.G., Kazantseva D.V., Vasilevа A.R., Ivanova S.A., Smirnova L.P.

Abstract

This work is devoted to the study of the association of MBP-hydrolyzing IgG activity with the level of antibodies to MBP in patients with schizophrenia. In the group of patients, a high level of specifi c proteolytic activity of antibodies (AT) and a twofold increase in the level of antibodies to MBP were found. A signifi cant positive correlation (r=0,48; p=0,026) was found between the studied parameters.

Russian Journal of Immunology. 2019;22(2-1):451-454
pages 451-454 views

INFLUENCE OF FOCAL INFECTION ON THE CYTOKINE PROFILE IN PATIENTS WITH CHRONIC DERMATOSIS

Patrushev A.V., Sukharev A.V., Nikitin V.Y., Ivanov A.M., Sukhina I.A., Gumilevskaya O.P.

Abstract

The aim of the study was to assess the impact of focal infection foci (FI) on the cytokine profile of patients with psoriasis, lichen planus (LP) and atopic dermatitis (AD). The patients were divided into two groups: those with FI (psoriasis – 19 people, AD – 13 people, LP – 14 people) and those without FI (psoriasis – 17 people, AD – 11 people, LP – 13 people). The control group was also examined – 15 healthy individuals. Immunoenzyme method was used to determine the following cytokines in the blood serum of patients: IL-1β, IL-2, IL-4, IL-6, IL8, IL-10, TNF-α, INF-α, INF-γ. It was shown that on the background of the presence of FI, the content of IL-8 in blood serum significantly increased (in 89,5% of cases in patients with psoriasis, in 76,6% in LP patients and in 100% in patients with AD).

Russian Journal of Immunology. 2019;22(2-1):455-458
pages 455-458 views

FEATURES CIRCULATING PRO- AND ANTITUMOR MONOCYTES IN PATIENTS WITH TRIPLE NEGATIVE BREAST CANCER

Patysheva M.R., Stakheyeva M.N., Fedorov A.A., Tarabanovskaya N.A., Grigorieva E.S., Slonimskaya E.M., Kzhyshkowska J.G., Cherdyntseva N.V.

Abstract

Phenotypic markers of pro- and antitumor polarization of blood monocytes in patients with a triple negative breast cancer were characterized in this work. This molecular subtype differed from the luminal B and Her2+ subtypes in the content of CD163+, stabilized-1+ and HLA-DR+ circulating monocytes. The identified differences can be used as a differential diagnostic marker for a triple negative breast cancer subtype.

Russian Journal of Immunology. 2019;22(2-1):459-461
pages 459-461 views

EFFECTS OF CARBOPLATIN WITH CUCURBIT[7]URIL ON SUBPOPULATION COMPOSITION AND PROLIFERATIVE ACTIVITY OF LYMPHOCYTES IN VITRO

Pashkina E.A., Aktanova A.A., Ermakov A.A., Knauer N.Y., Mirzaeva I.V., Kovalenko E.A.

Abstract

It is known that the use of delivery systems can reduce the toxicity of platinum compounds without losing a high therapeutic effect. In this work, the immunotoxic effect of carboplatin when co-added with cucurbit[7]uril was investigated, including the effect on the subpopulation composition of lymphocytes and their proliferative activity in vitro.

Russian Journal of Immunology. 2019;22(2-1):462-464
pages 462-464 views

SPONTANEOUS AND STIMULATED AMOUNT OF REGULATORY T-CELLS AND ACTIVATED T-HELPERS IN CHILDREN WITH DIFFERENT ACTIVITY OF JUVENILE IDIOPATHIC ARTHRITIS

Pashnina I.A.

Abstract

Children of 6‒17 years old with diff erent activity of juvenile idiopathic arthritis were examined: 42 with active arthritis, 11 with remission (with therapy), 10 children with persistent remission (without therapy) and 27 apparently healthy children. It was revealed by fl ow cytometry that after 24-hours incubation (37 °C, 5% СО2) the spontaneous and phytogemagglutinin-stimulated amount of regulatory T-cells (СD3+СD4+СD25+СD127) /neg) in all groups with juvenile idiopathic arthritis was decreased in comparison with healthy children. The number of activated T-helpers (СD3+СD4+СD25+СD127+) in all groups was the same. 

Russian Journal of Immunology. 2019;22(2-1):465-467
pages 465-467 views

DEFENSINES IN PATIENTS WITH ISCHEMIC STROKE

Pivovarova L.P., Osipova I.V., Ariskina O.B., Gogoleva E.A.

Abstract

The question of the importance of inflammatory and immune mechanisms of brain damage in cerebrovascular disease remains relevant.

The aim of our study was to assess the level of inflammation and the anti-bacterial potential of neutrophilic granulocytes (NG) in patients with ischemic stroke (IS). Surveyed 17 people with IS aged 68.5 (67; 79) years and 15 volunteers aged 65.0 (62.0; 66.5) years. The neurological status of the patients was assessed using the NIHSS and Rankin scales, Rivermid Mobility Index. Blood IL-6, CRP, cortisol, fibrinogen, soluble defensins (s Def) levels at 1, 3 and 10 days after IS was determined. α-Defensins in NG (def+ NG) were evaluated usingm Abs HNР 1–3 (NCL-Defensin). To assess the phagocytic activity of NG, the phagocytic index (PI) and phagocytic number (PN) were determined. Statistical processing of the research results was carried out using STATISTIKA 6.0.

Main results. Signs of systemic inflammation were observed in patients with IS blood levels of NG, IL-6, CRPand cortisol. The relationship of the somatic status of patients, the size of the lesion of the brain tissue, the severity of IS, the degree of disability, the outcome of IS with the content of NG and def+ NG was revealed. Phagocytosis inhibition in the dead was associated with a degree of disability. A positive association between def+ NG and NG (r = 0.659, p <0.001), IL-6 (r = 0.659, p ><0.001), cortisol (r = 0.573, p ><0.001), fi brinogen (r = 0.369, p >< 0.05). The data obtained indicate the development of systemic inflammation and an imbalance of the functional activity of NG in patients with IS, which are associated with the severity of cerebral stroke and create the risk of developing infectious complications. 

Russian Journal of Immunology. 2019;22(2-1):468-470
pages 468-470 views

CORRECTION OF IMMUNE ALTERATIONS IN PATIENTS WITH COMMUNITY-ACQUIRED PNEUMONIA

Polyakov D.V., Konoplya E.N.

Abstract

The problem of suitable management of the patients with community-acquired pneumonia requires new methods for solving. The conducted examination of the patients with moderate severity communityacquired bacterial pneumonia has showed that assigment of standard therapy did not allow to normalize most of the parameters refl ecting condition of the system complement and cytokines. In the group of patients, where traditional therapy was followed by addition of Derinat, Mexicor and Essentiale Forte H, was a significant decrease of more markers of immune infl ammation. The additional use of immunomodulatory, antioxidant and membrane-protective therapy is immunologically reasonable.

Russian Journal of Immunology. 2019;22(2-1):471-473
pages 471-473 views

ANTICYTOKINE ACTIVITY OF E.COLI STRAINS, ISOLATED FROM URINE WITH UROLITHIASIS

Popova L.P., Pashkova T.M., Morozova N.V., Kyz’min M.D., Kartashova O.L.

Abstract

A high level of prevalence and severity of anticytokine activity in E. coli isolated from the urine of ICD patients with postoperative inflammatory complications was established, an algorithm was developed for predicting the risk of their development using the data obtained.

Russian Journal of Immunology. 2019;22(2-1):474-476
pages 474-476 views

STRUCTURAL AND FUNCTIONAL CHARACTERISTIC OF CELLULAR ARM OF THE IMMUNE RESPONSE IN CHILDREN WITH BRONCHIAL ASTHMA

Prosekova E.V., Sitdikova T.S., Turyanskya A.I.

Abstract

We have studied the structural and functional parameters of cellular component of immune system in 120 children with verified diagnosis of bronchial asthma (BA) and 30 healthy children of the same age. To analyze cellular parameters, we used flow cytofluorometer COULTER EPICS XL by Beckman Coulter Inc. with statistical processing by Statistica 10 program with critical significance level p<0.05. As compared to the healthy children’s parameters, the children with BA showed higher level of Th17, decreased absolute number of lymphocytes, B- and T-lymphocytes, with the increase in the share of Tlymphocytes with the early and late activation markers. The studies performed identifi ed in children with BA the changes in the pattern of lymphocyte subpopulations and functional activity of T-lymphocytes which determine the immune response imbalance, activation of antigen recognition regulation, triggering and realization of immune response, lymphocyte proliferation and differentiation.

Russian Journal of Immunology. 2019;22(2-1):477-479
pages 477-479 views

THEINNATE IMMUNITY CELLS IN THE PATHOGENESIS OF TRAUMATIC BRAIN INJURY

Radkov I.V., Plekhova N.G., Zinoviev S.V., Shumatov V.B.

Abstract

The content of innate immunity cells expressing CD45, CD14, CD16, CD11b and F4 / 80 receptors brain in experimental modeling of traumatic brain injury (TBI) was studed. It was established that the number of CD16/11b positive macrophages of the M1 subpopulation increased in microgy on the 2nd day after TBI and signifi cantly enlarged up to 8 days. In the corpus callosum and the ipsilateral area of the striatum, CD16/11b expressed cells also peaked 8 days after TBI and correlated with an increase in the positive response to the presence of the endothelial antigen SMI71. Signifi cant structural transformations of the nervous tissue and the output of peripheral immune cells, coupled with impaired permeability by the blood-brain barrier, were shown. The disturbance of regulatory changes neuroimmune connections in TBI was proved.

Russian Journal of Immunology. 2019;22(2-1):480-481
pages 480-481 views

FEATURES OF CYTOKINE STATUS OF YOUNG MEN, PATIENTS WITH OSTEOARTHRITIS

Raymuev K.V., Malyshev M.E., Mazurov V.I., Bumay A.O., Habirova T.G.

Abstract

In young men with osteoarthritis, hyperproduction of proinflammatory cytokines is observed, along with a decrease in IL-2 production at the earliest stage of inflammation.

Russian Journal of Immunology. 2019;22(2-1):483-485
pages 483-485 views

HUMAN RECOMBINANT ANTI-MULLERIAN HORMONE: A SELF-ACTIVATING DRUG

Rak A.Y., Trofimov A.V., Ischenko A.M.

Abstract

Here, the autoproteolytic activity of human recombinant anti-Mullerian hormone (rAMH), a potential antineoplastic drug, was investigated. It was shown that the hormone is not only able to activate itself by the limited proteolysis, but also specifically interacts with the proteolytic inhibitor aprotinin. The involvement of the rAMH specific proteolysis site in interaction with a specifi c receptor type II (MISRII) was found. The data obtained may be useful to clarify some aspects of the native AMH biochemistry and pharmacodynamics of the recombinant hormone.

Russian Journal of Immunology. 2019;22(2-1):486-488
pages 486-488 views

EVALUATION OF VASCULAR ENDOTHELIAL FACTORS IN WOMEN WITH SELECTIVE DELAY OF FETUS GROWTH DURING MONOCHORIAL PREGNANCY

Remizova I.I., Chistyakova G.N., Aitov A.E.

Abstract

The urgency of the problem of monochorial multiple pregnancy is due to the significant frequency of development of selective intrauterine growth-restricted (sIUGR) of the fetus.

Objective: To evaluate the features of angiogenesis in women with fetal sIUGR during monochorial pregnancy. 46 pregnant women with monochorial diamniotic twins were examined: 21 women with fetal sIUGR and 25 women without gestational complications. It has been established that the implementation of fetal sIUGR in monochorial pregnancy is caused by an imbalance of the main regulators of angiogenesis, endothelial dysfunction and a decrease in the number of endothelial progenitor cells, which leads to a violation of the stabilization and integrity of the vascular endothelium. 

Russian Journal of Immunology. 2019;22(2-1):489-491
pages 489-491 views

METABOLISM OF CIRCULATING PHAGOCYTES IN PATIENTS WITH LUPUS ERYTHEMATOSUS

Romanov V.A., Erygina E.N., Romanova N.V., Kapreljanc E.Y.

Abstract

Inpatient with Systemic Lupus Erythematosus and Discoid Lupus Erythematosus oppression lisosomal activity of neutrophils and monocytes against a backdrop of increasing oxidative stress of cells.

Russian Journal of Immunology. 2019;22(2-1):492-493
pages 492-493 views

METABOLISM OF CIRCULATING PHAGOCYTES IN PATIENTS WITH SCLERODERMA

Romanov V.A., Erygina E.N., Romanova N.V., Kapreljanc E.Y.

Abstract

Systemic sclerosis, unlike the local sclerosis, stated the oppression of digestive ability of neutrophils and monocytes against a backdrop of increasing not oxidative metabolism phagocytes.

Russian Journal of Immunology. 2019;22(2-1):494-495
pages 494-495 views

SECRETION OF TUMOR NECROSIS FACTOR ALPHA BY BLOOD EOSINOPHILS IN COLON CANCER WITH TISSUE EOSINOPHILIA

Romanova E.V., Kolobovnikova Y.V., Urazova O.I., Novitskiy V.V.

Abstract

We analyzed in vitro secretion of TNFα by peripheral blood eosinophil granulocytes in patients with colon cancer according to the presence of tumor-associated tissue eosinophilia. In colon cancer with tissue eosinophilia, an increase in basal and r-IL-5-induced in vitro secretion of TNFα by blood eosinophils was detected. It was shown that in patients with colon cancer, regardless of the presence of tissue eosinophilia, the levels of basal and r-IL-5-induced secretion of TNFα by eosinophil granulocytes in vitro were comparable.

Russian Journal of Immunology. 2019;22(2-1):496-498
pages 496-498 views

APPLICATION OF GENOTYPING CYP3A5 POLYMORPHISM FOR CORRECTING DOSE OF TACROLIMUS IN KIDNEY TRANSPLANTATION

Ruzibakiyeva M.R., Aripova T.U., Hadzhibaev F.A., Sharipov V.K., Azizova Z.S.

Abstract

Currently, according to standard immunosuppression protocols after organ transplantation, tacrolimus is used as a part of immunosuppressive therapy. Along with obtaining the desired immunosuppressive effect, the problem of the development of unwanted adverse reactions to this drug is highly relevant. Pharmacogenetic testing of genotyping of the polymorphism of CYP3A5 G6986A, allows a personalized approach to the selection of the dosing regimen of tacrolimus, may increase the number of patients in whom the concentration of tacrolimus will be within the therapeutic range and will not exceed the acceptable toxicity level.

Russian Journal of Immunology. 2019;22(2-1):499-502
pages 499-502 views

MODULATING EFFECT OF EUKARYOTIC DNA ON THE EXPRESSION OF FUNCTIONALLY IMPORTANT RECEPTOR OF NEUTROPHILIC GRANULOCYTES

Rusinova T.V., Chudilova G.A., Kovaleva S.V., Tarakanov V.A., Barova N.K., Nesterova I.V.

Abstract

In article were studied phenotypic features of 2 neutrophilic granulocytes (NG) subsets expressing CD64, CD32, CD16, CD11b in children with systemic purulent-inflammatory diseases. We evaluated sodium salt of eukaryotic DNA effect on transformed phenotype of these NG subsets in vitro. Immunomodulatory effects of DNA have been established, which are manifested in a significant decrease in the number of NGs of the pro-inflammatory subset CD64+CD32+CD16+CD11b+NG, the decrease in the number of NG subset of CD64+CD32CD16+CD11b+ NG defective in CD32 and the increase the antiinflammatory subset CD64+CD32+CD16+CD11b+NG. 

Russian Journal of Immunology. 2019;22(2-1):503-505
pages 503-505 views

DETERMINATION OF THE LEVEL OF CYTOKINES IN BRONCHOALVEOLAR LAVAGE FLUID IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONAY DISEASE

Ryabova L.V., Nikonova S.E.

Abstract

19 patients were diagnosed with stage III–IV COPD, the smoking experience was 39.1±1.94 years. In this group of patients, a study of cytokines IL-1β, IL-6, IL-8, IL-10, TNF-α in bronchoalveolar lavage fluid was carried out by the method of enzyme-linked immunosorbent assay. Disequilibrium of cytokins was found, which was appeared by statistically significant raising of proinfl ammatory cytokines IL-1β, IL-6, IL-8, and statistically significant lowering for anti-inflammatory cytokines IL-10, so that proves presence of chronic persistent infl ammations in the bronchial tree.

Russian Journal of Immunology. 2019;22(2-1):506-508
pages 506-508 views

DETERMINATION OF THE LEVEL OF CYTOKINES IN BRONCHOALVEOLAR LAVAGE FLUID IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONAY DISEASE

Ryabova L.V., Nikonova S.E.

Abstract

19 patients were diagnosed with stage III–IV COPD, the smoking experience was 39.1±1.94 years. In this group of patients, a study of cytokines IL-1β, IL-6, IL-8, IL-10, TNF-α in bronchoalveolar lavage fluid was carried out by the method of enzyme-linked immunosorbent assay. Disequilibrium of cytokins was found, which was appeared by statistically significant raising of proinfl ammatory cytokines IL-1β, IL-6, IL-8, and statistically significant lowering for anti-inflammatory cytokines IL-10, so that proves presence of chronic persistent inflammations in the bronchial tree.

Russian Journal of Immunology. 2019;22(2-1):506-508
pages 506-508 views

ASSESSMENT OF CORRELATION BETWEEN CYTOKINE STATUS AND RED CELL DISTRIBUTION WIDTH IN PATIENTS WITH ATHEROSCLEROSIS OF LOWER LIMBS AFFECTED BY DIABETES MELLITUS TYPE 2

Savchuk K.S., Ryabova L.V., Nikushkina K.V., Nikonova T.I.

Abstract

Research’s objective is to make an analysis of correlations between index of systemic inflammation and red cell distribution width in patients with diabetes mellitus type 2 aff ected by atherosclerosis of lower limbs. 40 patients with diabetes mellitus were examined. Using Spearmen rank correlation, statistically signifi cant correlations between IL-1β and red cell distribution width were discovered (r=0,358; p=0,027). Herewith there is no signifi cant correlation between IL-6, IL-8, IL-10, TNF-α and red cell distribution width.

Russian Journal of Immunology. 2019;22(2-1):509-511
pages 509-511 views

TUMOR STEM CELLS AND THEIR MICRO-ENVIRONMENT: THE ROLE IN THE DEVELOPMENT OF THE TUMOR

Sagakyants A.B., Novikova I.A., Ulyanova E.P., Zolotareva E.I., Shaposhnikov A.V., Dzhenkova E.A.

Abstract

Cancer stem cells (CSC) are a special functional group of cells in a heterogeneous tumor population, causing both the maintenance and growth of a tumor, metastasis processes, and resistance to various chemotherapeutic drugs. The questions of a comprehensive study of the dynamics of the structural and functional organization of CSC niches, the role of its individual components in maintaining these cells and prognosis of their future behavior remain topical. This paper is devoted to the consideration of the biological properties of cancer stem cells, their interaction with some components of their microenvironment and the role of this interaction in maintaining the properties of CSC.

Russian Journal of Immunology. 2019;22(2-1):512-514
pages 512-514 views

IN HIV-INFECTED PATIENTS, DISCRORDANT RESPONSE OF CD4+ T-CELLS TO ANTIRETROVIRAL THERAPY IS ASSOCIATED WITH ENERGY METABOLISM VIOLATION

Saidakova E.V., Shmagel K.V., Korolevskaya L.B., Shmagel N.G.

Abstract

We evaluated the ability of memory CD4+ T-cells that were isolated from the blood of HIV-infected subjects with a discordant response to antiretroviral therapy to proliferate. It was shown that in those patients, frequency of dividing CD4+CD45RA– T-cells was increased against the background of CD4+ T-cell deficiency. However, those lymphocytes were not able to complete the cell cycle and productively divide. Furthermore, in cycling memory CD4+ T-cells violation of mitochondria and energy metabolism were detected. 

Russian Journal of Immunology. 2019;22(2-1):515-517
pages 515-517 views

LEADING MECHANISMS OF IMMUNOPATHOGENESIS IN VARIOUS TYPES OF INFLAMMATION (INFECTIOUS, AUTOIMMUNE, ATOPIC)

Salmasi J.M., Poryadin G.V., Kazimirsky A.N.

Abstract

The purpose of this study was to identify the leading mechanisms of immunopathogenesis of inflammation diseases of different genesis. Using the indirect immune fluorescence method, the number of lymphocytes carrying surface receptors CD3, CD4, CD8, CD16, CD56, CD20, CD72, mIgM, mIgG, CD23, CD25, CD71, HLA-DR, CD54, CD95, CD30, CD38, CD178. Lymphocytes of patients with aseptic inflammation showed an increase in the number of CD25, CD71, and HLA-DR lymphocytes – markers of activation. But the complete absence of lymphocyte activation was found in severe cases of infectious inflammatory process in patients with osteomyelitis. In autoimmune inflammation, the lymphocytes activation process has a high intensity and is accompanied by a weakening of the expression of the activation receptor apoptosis CD95. Atopic diseases (atopic dermatitis and atopic bronchial asthma) are characterized by an intense lymphocytes activation process, which is accompanies with a significant decrease in the number of CD95+ lymphocytes, but different by another lymphocytes subpopulations. 

Russian Journal of Immunology. 2019;22(2-1):518-520
pages 518-520 views

FEATURES OF THE PRODUCTION OF INTERLEUKIN-5 IN ATHEROSCLEROSIS OF THE BRACHIOCEPHALIC ARTERIES

Saranchina Y.V., Kilinа O.Y., Dutova S.V., Pol’shcha N.G., Kulakova T.S., Chanarin N.V., Shandakov P.I.

Abstract

The article presents the results of interleukin-5 (IL-5) determination in patients with atherosclerosis (AS) of brachiocephalic arteries. It was found that the content of IL-5 in the serum of AS patients was signifi cantly lower than in the control group. Spontaneous and PHA-induced IL-5 production determined in blood leukocyte culture was signifi cantly lower in the group with as compared to the control group. Reduction of reserve capacity for IL-5 production by blood leukocytes was noted in both groups. Comparative analysis of IL-5 content in blood serum of AS patients and in atherosclerotic plaque homogenate did not reveal statistically signifi cant diff erences.

Russian Journal of Immunology. 2019;22(2-1):521-523
pages 521-523 views

ANTIMICROBIAL PEPTIDES IN THE TREATMENT OF MUSCULAR ORAL CAVITY

Sarkisyan N.G., Kataev N.N., Tuzankina I.A., Melikyan S.G.

Abstract

Antimicrobial peptides are the most promising drugs in the replacement of antibiotic therapy. The results of the analysis of the physicochemical properties of Atsegram spray, in particular, surface tension (σ, Erg / cm2) and conductivity (æ, μS / cm) show the relationship with the mechanism of the antibacterial action of the drug and its eff ectiveness in the treatment of periodontitis. Spray application revealed an improvement in the hygienic condition of the oral cavity by a factor of 1.4, a decrease in the PMA index by 1.8 times, a decrease in the bleeding index of the gingival margin by a factor of 2 a month after treatment compared with the same indicators of the comparison group undergoing standard treatment.

Russian Journal of Immunology. 2019;22(2-1):524-526
pages 524-526 views

TRITERPENOID MILIACINE AS A PROTECTOR OF CHROMOSOMAL ABERRATIONS INDUCED BY CYCLOPHOSPHAMIDE IN MICE BONE MARROW CELLS

Sarycheva Y.A., Tokareva A.A., Panfilova T.V., Zheleznova A.D., Frolov B.A.

Abstract

A study of the antimutagenic eff ect of miliacin, assessed by indicators of chromosomal aberrations in mice bone marrow cells under the conditions of cyclophosphamide use, was carried out. Triterpenoid protected the mutagenic action of cyclophosphamide by the signifi cant reduction of the relative content of aberrant cells and the number of aberrations, which makes it possible to evaluate it as a promising antimutagenic agent.

Russian Journal of Immunology. 2019;22(2-1):527-529
pages 527-529 views

EFFECTS OF GENETIC FACTORS TO INHALED CORTICOSTEROID RESPONSE IN CHILDREN WITH ASTHMA: A LITERATURE REVIEW

Serezhkina A.V., Bogomazov A.D., Polonikov A.V.

Abstract

Numerous studies have studied the Association between pharmacogenetic eff ects and response to inhaled glucocorticosteroids (ICS) in patients with asthma. In fact, several single nucleotide polymorphisms of a number of candidate genes have been identifi ed that may aff ect the clinical response to ICS in children with atopic asthma (BA). The results obtained to date indicate various potential genetic factors associated with the reaction to IGCs, which can be used to predict the individual therapeutic response of children with atopic asthma to ICS.

Russian Journal of Immunology. 2019;22(2-1):530-531
pages 530-531 views

EFFECTS OF INTERFERONOTHERAPY IN THE TREATMENT OF ATOPIC DERMATITIS WITH ACCOMPANYING SECONDARY IMMUNE INSUFFICIENCY SYNDROME

Sizyakina L.P., Penechko E.M., Andreeva I.I.

Abstract

The low effectiveness of standard basic therapy, the presence of clinical and laboratory signs of secondary immune deficiency in children with atopic dermatitis to determine the need of the appointment of immunocorrective therapy in these patients. It was shown that in children of the younger and older age groups, who used IFN-α drugs together with the standard therapy, the results of the treatment showed better results than in the subgroups that are only on basic therapy.

Russian Journal of Immunology. 2019;22(2-1):533-535
pages 533-535 views

FEATURES OF FOOD HYPERSENSITIVITY IN CHILDREN WITH AUTISM SPECTRUM DISORDER

Skirnevskaia A.V., Cherevko N.A., Khudiakova M.I., Kondakov S.E.

Abstract

According to world statistics, the incidence of autism spectrum disorders (68–100 cases per 1000 births) continues to increase in the children’s population, with boys predominating over girls (6–8: 1) [4]. Despite numerous studies on this problem, the pathogenesis of autism remains controversial. The purpose of this work is to study the clinical and immunological interactions of IgG mediated food hypersensitivity, cytokine balance and psychological parameters in children with autism spectrum disorders living in Tomsk. As a result, it was noted that children with autism are statistically significantly more often hypersensitive to cereals and dairy. Revealed changes in the concentration of a number of interleukins in the serum. A direct correlation was found between the concentration of IgG to antigens of the fungi of the genus Candida, food AG of dairy and grain and the concentration of IFNγ in serum.

Russian Journal of Immunology. 2019;22(2-1):536-538
pages 536-538 views

INDICATORS OF MUKOSAL IMMUNITY IN OFTEN ILL CHILDREN OF THE TREATMENT BY THERAPEUTIC VACCINE «IMMUNOVAC VP-4»

Slatinova O.V., Serova T.A., Bisheva I.V., Foshina E.P.

Abstract

Summary, immunoglobulin profile and specific antibodies to antigens of S. aureus and Kl. pneumoniae in saliva of often children with chronic hotbeds of bacterial infection ENT-organs was estimated. As a result, treatment of the drug «Immunovac VP-4» observed a statistically significant increase of concentration of IgG and sIgA and specific bacterial antibodies and-isotype as well as increase to normal values Ig A.

Russian Journal of Immunology. 2019;22(2-1):539-541
pages 539-541 views

SEVERAL EFFECTS OF TIMOGEN NEW ANALOGUE

Smakhtin M.Y., Chulanovа A.A., Koroshvili V.T., Smakhtina A.M.

Abstract

It has been established that in cases of skin wounds the timogen analogue, modified by D-alanin ‒ H2N-L-Glu-L-Trp-D-Ala-COOH, does not affect the values of the absorptive phase of blood neutrophil phagocytosis, superoxide dismutase activity and the number of lymphocytes in skin, comparing with timogen and similar with it. Peptides were injected ten days intraperitoneally in the same molar dosages 1 μg/kg for timogen and 1.2 μg/kg for it’s analogue. This peptide has more pronounced action, comparing with timogen, on stimulation of oxygen dependent neutrophil activity (NST-test) and on increase in the number of fi broblasts in skin.

Russian Journal of Immunology. 2019;22(2-1):542-544
pages 542-544 views

ESTIMATION OF IMMUNOLOGICAL INDICATORS IN RATS BORN FROM PASSIVALLY SMOKED MALE. COMMUNICATIONS1

Smolagin A.I., Mikhailova I.V., Ermolina E.V., Pushkareva L.A., Isengulova A.A., Kuzmicheva N.A., Miroshnichenko I.V.

Abstract

The results of changes in the immunological parameters of rat pups born to females, which during pregnancy were exposed to passive smoking and the effects of immunomodulators KK1 and neurokinin A, are presented.

Russian Journal of Immunology. 2019;22(2-1):548-550
pages 548-550 views

APPLICATION OF BODY-ORIENTED PSYCHO-CORRECTION IN THE BRONCHIAL ASTHMA COMPLEX THERAPY

Smyk A.V.

Abstract

Asthma is a chronic relapsing disease. Psychological factors have a signifi cant influence on the development and course of asthma, which is why the disease is considered to be a psychosomatic disease. An important role in the pathogenesis of this disease is given alexithymia, and therefore the purpose of the study was to assess the effectiveness of psychological correction of alexithymia in the complex therapy of asthma. There was a reduction in the level of alexithymia to the standard ratios and improve lung function in patients undergoing drug therapy with kinesiology psychocorrection. The most pronounced eff ect on these indicators registered in patients with a dominant left hemisphere of the brain. The next three months in these patients achieved complete asthma control. Our results demonstrate the positive therapeutic effect of kinesiology psychocorrection in patients with asthma that determines the feasibility of its inclusion in the complex therapy of said disease.

Russian Journal of Immunology. 2019;22(2-1):551-553
pages 551-553 views

CHARACTERISTICS OF THE INTERFERON SYSTEM AND CELLULAR IMMUNITY OF THE CHILDREN WITH FIRST DIAGNOSED ACTIVE PULMONORY TUBERCULOSIS

Sokolova T.F., Mordyk A.V., Shcheglova E.L., Sokol N.N., Aroyan A.R.

Abstract

The article reveals the features of changes in the system of interferon and cellular immunity, structural and functional organization of the immune system by the number of correlations between its elements among the children with pulmonary tuberculosis. The article gives an analysis of secretion of IFNα and IFNγ, subpopulation composition of blood lymphocytes, communication in the immune system. It is stated that hypo secretion of IFNγ by lymphocytes in vitro with directed induction of cells is registered against the background of a decrease in the number of CD3+, CD4+, CD8+, CD16+, CD20+, HLA-DR+ lymphocytes, an increase in the number of connections between elements of the immune system with a decrease in its mobility.

Russian Journal of Immunology. 2019;22(2-1):554-556
pages 554-556 views

IMMUNOGENICITY AND SAFETY OF BIOTECHNOLOGICAL MEDICINES

Soldatov A.A., Avdeeva Z.I., Bondarev V.P., Merkylov V.A.

Abstract

The efficacy and safety of the use of biotechnological (biotherapeutic, genetic engineering) preparations are due to their targeted mechanism of action and low of toxicity, in contrast to traditional chemicals drugs. These features of biotherapeutic preparations also aff ect their safety profile. When treating with biotherapeutic preparations, the most major adverse reaction and the first developmental time is the so-called infusion reaction. The basis of the development of the reaction to the infusion of a biotherapeutic preparation may be one of the following reactions: cytokine release syndrome (CRS), type I hypersensitivity reaction (IgE-dependent allergic reaction) or IgG-mediated reaction. These reactions have a similar clinical picture, so it is difficult to clearly determine which mechanisms led to the development of the reaction to the infusion. The mechanisms of development of the reaction to the infusion are still poorly studied.

Russian Journal of Immunology. 2019;22(2-1):557-559
pages 557-559 views

THE RELATIONSHIP OF INFLAMMATION, ENDOTHELIAL DYSFUNCTION AND CELLULAR LINK HEMOSTASIS IN PATIENTS DURING THE RECOVERY PERIOD OF STROKE

Soloveva E.Y., Baranova O.A., Chekanov A.V., Tyutyumova E.A., Fedin A.I.

Abstract

A study of induced platelet aggregation, the level of markers of immune inflammation, endothelial dysfunction in patients during the recovery period of stroke. It has been established that both in the early and in the late post-stroke period, signs of chronic immune inflammation associated with endothelial dysfunction persist. The risk groups for recurrent stroke in patients during the recovery period were identified.

Russian Journal of Immunology. 2019;22(2-1):560-562
pages 560-562 views

DIFFERENTIATION OF CD8+ LYMPHOCYTES AND THEIR CYTOLYTIC ACTIVITY IN WOMEN WITH THREATED PRETERM LABOR

Sotnikova N.Y., Borzova N.Y., Radjabova N.R.

Abstract

The aim of this work was to determine the features of diff erentiation of effector cytotoxic T lymphocytes (CTL) in women with threated preterm labor. Under observation were 100 pregnant women of 24–34 weeks of pregnant: the main group consisted of 44 women with threated preterm labor, control group – of 56 women with uncomplicated course of gestation. The relative content of CD8 cells, CD8 naïve cells (Tn), CD8 memory cells: Central (Tcm), preterminal-differentiated (Tem) and terminal differentiated (Temra) was studied in perephiral bloded. The content of CD8 cells producing Perforin was assessed in the total population of lymphocytes (CD8 Perf), as well as in populations of CD8 Tcm, Tem, Temra cells. The results of the study showed that women with threated preterm labor was characterized by a significant increase in the relative content of CD8 and CD8 Perf+ lymphocytes. The content of naive cells was significantly increased in the population of CD8 CTLs in women with exercise. In addition, there was a significant increase compared to that in control group the content of Perforin-producing cells in the fractions of Tcm, Tem and Temra cell memory. 

Russian Journal of Immunology. 2019;22(2-1):563-565
pages 563-565 views

EXPERIMENTAL MICE SEPSIS MODELS: ADVANTAGES AND PITFALLS

Starkina O.V., Ilyukina N.A., Vassilev T.L.

Abstract

Sepsis is studied in vivo by using experimental mice models. The golden standard is the model which utilizes peritonitis induction by cecal ligation and puncture. Lipopolysacharide injection is often used for induction of septic process as well. Currently, the researches discuss disadvantages of these models and poor modeling capability of mice sepsis compared to septic processes which take place in humans. The throughout discussion is present in the article by Cavaillon J. M. “New Approaches to Treat Sepsis: Animal Models Do Not Work” [1]. In this review the author questioned the sepsis animal models used nowadays, but he didn’t mentioned the experimental model described by Gonnert F. A. et al. in their work «Characteristics of Clinical Sepsis Refl ected in a Reliable and Reproducible Rodent Sepsis Model». Our review briefl y discusses the advantages of this model compared to the other animal sepsis models.

Russian Journal of Immunology. 2019;22(2-1):566-568
pages 566-568 views

CONTRIBUTION OF PERIPHERAL BLOOD MONOCYTES IN THE FUNCTIONAL STRATEGY OF THE IMMUNE SYSTEM IN BREAST CANCER

Stakheyeva M.N., Patysheva M.R., Tarabanovskaya N.A., Kzhyshkowska J.G., Cherdyntseva N.V.

Abstract

The phenotypic and expression characteristics of peripheral blood monocytes were evaluated in relationship to the antitumor and tumor-stimulating state of the immune system. It has been shown that the favorable state of the immune system associated with prolonged clinical remission is characterized by a higher level of functional activation of peripheral blood monocytes, which is manifested in a more pronounced expression of the TNFα and YLK-39 genes and a higher proportion of TLR2+ monocytes compared with the corresponding indicators in patients with unfavorable status. 

Russian Journal of Immunology. 2019;22(2-1):569-571
pages 569-571 views

EVALUATION OF SINGLE NUCLEOTIDE POLYMORPHISMS -819C/T, -592C/A IL-10 IN PATIENTS WITH RHEUMATOID ARTHRITIS AS A GENETIC RISK FACTOR

Stashkevich D.S., Suslova T.A., Burmistrova A.L.

Abstract

The frequency distribution of the occurrence of alleles, genotypes and combinations of genotypes formed by SNPs -819, -592 IL-10 was assessed in this research. It was established that in the group of patients with RA, the distribution corresponds to that in European populations. No association of genetic polymorphism of SNPs -819, -592 IL-10 with a predisposition to RA in the Russians of the Chelyabinsk region was found.

Russian Journal of Immunology. 2019;22(2-1):572-574
pages 572-574 views

ANTI-TUMOR AND IMMUNOMODULATING EFFECTS OF RECOMBINANT HUMAN TUMOR NECROSIS FACTOR ALPHA IN A DELIVERY SYSTEMIN B16-F10 MELANOMA MICE

Sysoeva G.M., Ryabchikova E.I., Gamaley S.G., Lebedev L.R., Volosnikova E.A., Danilenko E.D.

Abstract

It was established that recombinant human tumor necrosis factor alpha in a delivery system has pronounced antitumor and immunomodulating effects in murine B16-F10 melanoma model. The preparation significantly inhibited the growth of experimental tumor (TGI 60%), increased tumor infiltration by immune system cells (CD3+, CD11b+) and increased the activity of immunocompetent cells mediating antitumor response.

Russian Journal of Immunology. 2019;22(2-1):575-577
pages 575-577 views

PREVALENCE AND REGIONAL PECULIARITIES OF POLINNOSIS

Tarabrina O.V., Yudina S.M., Korshikova M.Y., Rusanova T.S.

Abstract

The article analyzes the spectrum of sensitization and presents the results of a study of the cytokine profi le, indicators of cellular and humoral immunity in residents with pollinosis of the Kursk region.

Russian Journal of Immunology. 2019;22(2-1):578-580
pages 578-580 views

LEVEL OF MATRIX METAL PROTEINAS AND THEIR TISSUE INHIBITORS IN WOMEN WITH NON-CARE OF PREGNANCY

Tulupova M.S., Hamoshin M.B., Nevezhkina T.A., Sidorova O.N.

Abstract

Surveyed 28 patients with sexually transmitted infections (STIs), or papillomavirus infection (PVI). The patients were divided into the main group (FG) and the control group (GC) (n = 10). The main group was divided into 2 groups: Group I (n = 8) ‒ STIs with MWI, Group II (n = 10) ‒ STIs without MWI. A simultaneous increase in MMP-9 and TIMP-1 in the serum of women with miscarriage was established, which indicates the role of the processes of damage to the extracellular matrix in its pathogenesis. Diff erences in the ratio of MMP-9 to TIMP-1 depending on the presence of HPV were revealed. Women with STIs without HPV are dominated by products of TIMP that can have a profibrinogenic eff ect.

Russian Journal of Immunology. 2019;22(2-1):581-583
pages 581-583 views

FEATURES OF CYTOKINES, ADIPOKINES, MATRIX MATELOPROTEINASE-9 AND ITS INHIBITORSIMBALANCE, DEPENDING ON THE CLINICAL MANIFESTATIONS OF ATHEROSCLEROSIS

Turmova E.P., Markelova E.V., Kiku P.F.

Abstract

140 patients with coronary atherosclerosis (CA), ischemic heart disease (IHD) and 120 people with obliterating atherosclerosis of lower extremities (OALE) were examined. The levels of IL-1β, IL-6, IFNγ, TNFα, IL-10, TGF-β1, TGF-β2, IL-2, IL-17, soluble cytokine receptors: IL-2R, IL- 6R, TNFα RI and TNFα RII, metalloproteinase-9 and its inhibitors (MMP-9, TIMP-1, MMP-9/TIMP-1, MMP-9/TIMP-2 complexes), adipokines (leptin and adiponectin) in serum. In patients with OALE, increase of IFNγ, TNFα, TN-α RII, IL-6 and TIMP-1 (p<0.05) was registered. By the factor analysis method, it was determined, that the IFNγ, IL-2, TNFα and IL-6 have leading pathogenetic significance in OALE. In patients with IHD, pathogenetic signifi cance and increase of the IL-1β, IL-17 and increase of the leptin was revealed.

Russian Journal of Immunology. 2019;22(2-1):584-586
pages 584-586 views

PECULIARITIES OF THE CYTOKINE PROFILE IN CHILDREN WITH BRONCHIAL ASTHMA IN COMBINATION WITH ALLERGIC RHINITIS

Turyanskya A.I., Sabynych V.A.

Abstract

We have studied the content of pro-inflammatory and anti-inflammatory cytokines (IL-4, IL-10, IL-13) and level of IFNγ ‒ production in blood serum of 110 children with bronchial asthma aged 3‒11 years old. The control group consisted of 30 healthy children of the same age. The said cytokines were analyzed in blood serum using sandwich ELISA technique and chemical reagents by R&D Diagnostics Inc (USA). Both during remission and exacerbation, levels of IL-4, IL-10, IL-13 in children with BA was found to be signifi cantly higher, and the level of IFNγ – considerably lower. The identifi ed tendency is also typical for children with combined bronchial asthma and allergic rhinitis.

Russian Journal of Immunology. 2019;22(2-1):587-589
pages 587-589 views

EARLY CYTOKINE PREDICTORS OF ACUTE REJECTION OF KIDNEY TRANSPLANT

Fedorenko T.V., Kolesnikova N.V., Pashkova I.A.

Abstract

The study of the protective role of blood cytokines evaluated by multiplex analysis before kidney transplantation in recipients with chronic kidney disease showed that the predictors of acute kidney transplant rejection in recipients can be considered high plasma levels of IL-17A, IL-1RA, IL-33, CCL2, as well as BDNF and ccl1 defi ciency. The early signs of a favorable post-transplant period are complete absence or extremely low levels of IL-1β, IL-6, IL-17A, decreased levels of LIF, IL-33, CXCL12), as well as high levels of CXCL8, CXCL10, IFNγ and CCL5.

Russian Journal of Immunology. 2019;22(2-1):590-591
pages 590-591 views

THE EFFECT OF ALLOIMMUNIZATION BY HUMAN LYMPHOCYTES ON THE PROLIFERATIVE ACTIVITY OF WOMAN CELLS WITH A HIGH FREQUENCY OF HLA SHARING

Fedorova A.V., Khonina N.A.

Abstract

The level of the proliferative response of female cells to partner antigens in women with a high degree of HLA sharing in couples is significantly lower than in women with no HLA similarity. The alloimmunization of the partner’s lymphocytes is accompanied by an increase in the proliferative response in all groups of women regardless of HLA sharing. However, woman with a high sharing of HLA antigens have a lower level of cell proliferation than woman with no sharing of HLA antigens.

Russian Journal of Immunology. 2019;22(2-1):593-595
pages 593-595 views

ALTERNATIVE WAYS TO ACTIVATE THE CHEMOTAXIS OF ACUTE MYELOID LEUKEMIA CELLS

Filina A.B., Svitich O.A., Golenkov A.K., Klinushkina E.F., Zverev V.V.

Abstract

One of the main factors of innate immunity involved in tumor progression and metastasis are TLRs, ligands of TLRs, chemokines and their receptors. The relationship between the above factors and the oncological process was studied not revrntly, but the last decade has been actively studied by scientists from all over the world. Despite the great interest in this issue, a more detailed study so far concerns only a few chemokines, such as CXCL12, CCL8 and their receptors, as well as TLRs and their ligands. Thus, in our work, we wanted to explore possible options for mediated chemotaxis of leukemia cells, as well as the eff ect of heterologous receptor activation on each other.

Russian Journal of Immunology. 2019;22(2-1):596-598
pages 596-598 views

LEVELS OF IL-6 AND IFN-γ IN CHILDREN WITH SCHIZOPHRENIA AND AUTHISM SPECTRUM DISORDERS WITH DIFFERENT PHENOTYPES OF SOCIAL BEHAVIOR

Filippova Y.Y., Burmistrova A.L.

Abstract

The paper presents the results of a study of the levels of important regulatory mediators of neurogenesis and neuroplasticity: IL-6 and IFN-γ in the blood plasma of children with schizophrenia and autism spectrum disorders with diff erent phenotypes of social behavior. Children with schizophrenia have been found to have signifi cantly high levels of the cytokines, both in comparison with typically developing children and with children with autism. The heterogeneous group of children with autism, a feature of which is the partial loss of previously acquired social skills – «Regression», is characterized by intermediate IL-6 and IFN-γ values and, as we assume, can include children with schizophrenia.

Russian Journal of Immunology. 2019;22(2-1):599-601
pages 599-601 views

INFLUENCE OF MILIACIN TRITERPENOID ON ANTILYSOCIMATIC ACTIVITY OF MICROORGANISMS

Filippova Y.V., Bondarenko T.A., Panfilova T.V., Zheleznova A.D., Sarycheva Y.A., Tokareva A.A.

Abstract

The inhibitory effect of triterpenoid miliacin in a dose of 50 μg / ml on the persistent trait of microorganisms – antilysocyme activity was established. The revealed property of militia along with its immunomodulating activity can provide a previously identified protective effect in experimental Salmonella infection.

Russian Journal of Immunology. 2019;22(2-1):602-604
pages 602-604 views

THE INTEGRATED METHODOLOGY OF CONTRASTING HARDENING FOR THE CHILDREN AGED 4–6

Fisher T.A., Kalenova L.F., Kolyvanova S.S.

Abstract

The complex method of contrasting hardening for children of preschool age is described by clinical, immunological and psychoemotional parameters with an indication of the sequence of stages of contrast procedures.

Russian Journal of Immunology. 2019;22(2-1):605-607
pages 605-607 views

BENEFITS OF COMBINATE VACCINI THERAPY IN CHILDREN WITH RECCURENT INFECTIONES IN IMMUNOLOGIST OPINION

Foshina E.P., Slatinova O.V., Serova T.A., Konanyhina S.U., Afinigenova V.P.

Abstract

Combinate therapy with oral bacterial lysates and pneumococcal vaccine in children with recurrent infections provides more increased immunomodulating infl uence to factors of adaptive, primal and mucosal immunity.

Russian Journal of Immunology. 2019;22(2-1):608-610
pages 608-610 views

STANDARD TREATMENT IN THE CORRECTION OF IMMUNE DISTURBANCES IN ACUTE SEROUS AND PURULENT PYELONEPHRITIS

Kholimenko I.M., Shatohin M.N., Maltsev V.N., Kotsar A.G., Emelyanova E.S.

Abstract

Objective: to study the eff ectiveness of the basic treatment of acute pyelonephritis in terms of immune status.

Materials and methods. The immune status of 62 patients with various forms of acute pyelonephritis.

Results. and its discussion. When serous and purulent forms of acute pyelonephritis are observed: increased levels of cytokines with a decrease in anti-infl ammatory with purulent pyelonephritis and their imbalance ‒ with serous form, an imbalance in the complement system, increased production of immunoglobulins with a simultaneous decrease in CIC, reduced activity and intensity of phagocytosis in the background of increased metabolic activity.

Conclusion. With serous and purulent forms of pyelonephritis, there are diff erences in the direction and severity of changes in immune parameters that do not normalize after treatment, which requires the development of additional methods of immunocorrection and immunorehabilitation. 

Russian Journal of Immunology. 2019;22(2-1):611-613
pages 611-613 views

EXPRESSION AND FUNCTIONAL ACTIVITY OF TLR2 AND TLR4 IN PATIENTS WITH ALLERGIC BRONCHIAL ASTHMA

Khoreva M.V., Latisheva T.V., Ogurtsova A.D., Gracheva L.A., Zakharov M.V., Switich O.A., Gankovskaya L.V.

Abstract

TLR2 and TLR4 expression and functional activity in peripheral blood mononuclear cell of patients with controlled allergic bronchial asthma were increased. TLR2 and TLR4 expression and functional activity in peripheral blood mononuclear cell were higher in patients with moderate asthma than in patients with mild asthma. The identifi ed changes may increase the susceptibility of patients to infectious agents and lead to complications.

Russian Journal of Immunology. 2019;22(2-1):614-616
pages 614-616 views

HLA-DRB1* ASSOCIATIONS WITH SPORADIC SEPTAL CONGENITAL HEART DISEASES WITHOUT CHROMOSOMAL DISEASES

Tsepokina A.V., Deev N.S., Shmulevich S.A., Shabaldin A.V., Korostelev A.A., Ponasenko A.V.

Abstract

Associations of HLA-DRB1* alleles were determined with sporadic septal congenital heart defects in children. 97 children (44 boys and 53 girls) with an isolated ventricular septal defect (VSD) without chromosomal diseases and anomalies were examined. Surveyed 132 healthy children (control group). The frequency of occurrence of 14 alleles of the HLA-DRB1 gene (01, 04, 07, 08, 09, 10, 11, 12, 13, 14, 15, 16, 17, 18). Mathematical processing was performed using the STATISTICA 8.0 application package (StatSoftInc., USA). It was revealed that in children with sporadic CHD without chromosomal diseases, HLA-DRB1*15 (p = 0.03) was signifi cantly more frequent in the genotype compared with the control group. The formation of sporadic septal congenital heart defects without chromosomal diseases is positively associated with HLA-DRB1*15.

Russian Journal of Immunology. 2019;22(2-1):617-619
pages 617-619 views

INTERRELATION OF INFLAMMATORY AND IMMUNE CHANGES IN CHRONIC ENDOMETRITIS

Tsiguleva O.A., Barsukova N.I., Obukhova L.E.

Abstract

Chronicity of inflammation in chronic endometritis is associated with an inadequate response of the immune system. The accumulation of special complexes in neutrophils – infl ammasm is accompanied by the release of pro-inflammatory cytokines, which deepen infl ammation. Only complex therapy: antibiotics, nonsteroidal anti-infl ammatory drugs, immunomodulators, enzyme therapy for 2‒3 months can lead to remission in chronic endometritis.

Russian Journal of Immunology. 2019;22(2-1):620-622
pages 620-622 views

EFFECT OF POTENTIAL ANTHELMINTIC DRUGS ON IL-1β EXPRESSION IN EXPERIMENTAL OPISTHORCHIASIS

Tsyganov M.A., Lvova M.N., Dushkin A.V., Modvinov V.A., Avgustinovich D.F.

Abstract

Interleukin-1 beta (IL-1β) is an indicator of liver infl ammatory processes in opisthorchiasis. Administration of curcumin and new complex of praziquantel with disodium glycyrrhizin ate reduced the parasitic load in O. felineus-infected animals. Moreover, the complex prevented the development of pathomorphological changes and reduced the IL-1β expression in the liver.

Russian Journal of Immunology. 2019;22(2-1):623-625
pages 623-625 views

INFLUENCE OF CYTOKINES ON BIOFILM FORMATION OF INTESTINAL MICROSYMBIONTS

Chainikova I.N., Ivanova E.V., Bondarenko T.A., Perunova N.B., Chelpachenko O.E., Nikiforov I.A.

Abstract

The multidirectional effect of recombinant cytokines (TNF-α, IL-10) on the biofilm formation of intestinal microsymbionts was established, which depended on the concentration of cytokines, the type and strain of microorganisms.

Russian Journal of Immunology. 2019;22(2-1):626-628
pages 626-628 views

CHANGES OF IMMUNE STATUS OF PATIENTS WITH DIFFERENT TYPES OF GLIAL TUMORS

Chemodakova K.A., Martynov B.V., Sukhina I.A., Nikitin V.Y., Ivanov A.M.

Abstract

The immune status of 58 patients with gliomaswas evaluated. Prior to surgical treatment in patients with low-grade gliomas was observed higher (p<0.05) the absolute number of total T-lymphocytes, Thelper cells and NK-cells compared to patients with high grade glioma. Postoperatively group grade II was observed explicit reduction in the number of CD3+, CD3+CD4+, CD3–CD56+ and increase in the population CD3+CD8+ cytotoxic T-lymphocytes. Reducing of the number of CD3–CD56+ lymphocytes in patients with diff use astrocytomas and anaplastic gliomas after surgical treatment, maybe, related to the migration of these cells into the area of surgical intervention. At the same time in patients with grade III, IV gliomas was revealed an increase of the absolute number of killer T-cells. In the group of grade IV also was recorded increase in the percentage of T-reg, having a suppressor function, which may indicate a breach of anti-tumor immune response and cause suppression of the cytotoxic response in patients with highgrade gliomas. Key words: glioma, low grade, high grade, immunophenotyping, immune status, T-lymphocytes, NK-cells>˂0.05) the absolute number of total T-lymphocytes, Thelper cells and NK-cells compared to patients with high grade glioma. Postoperatively group grade II was observed explicit reduction in the number of CD3+, CD3+CD4+, CD3CD56+ and increase in the population CD3+CD8+ cytotoxic T-lymphocytes. Reducing of the number of CD3CD56+ lymphocytes in patients with diffuse astrocytomas and anaplastic gliomas after surgical treatment, maybe, related to the migration of these cells into the area of surgical intervention. At the same time in patients with grade III, IV gliomas was revealed an increase of the absolute number of killer T-cells. In the group of grade IV also was recorded increase in the percentage of T-reg, having a suppressor function, which may indicate a breach of anti-tumor immune response and cause suppression of the cytotoxic response in patients with highgrade gliomas. 

Russian Journal of Immunology. 2019;22(2-1):629-631
pages 629-631 views

ENDOTELIOPROTECTIVE EFFECT OF INTRAVENOUS IMMUNOGLOBULINS IN OBSTETRIC PATHOLOGY. METHODOLOGICAL APPROACHES

Chepanov S.V., Shlachtenko T.N., Kornyushina E.A., Chudotvorov K.N., Orlova E.S., Selkov S.A.

Abstract

Intravenous immunoglobulins are investigated as cytoprotectors that can eff ectively protect endothelial cells from diff erent cytopathic factors contained in blood serum. We developed a method to evaluate the endothelioprotective eff ect of intravenous immunoglobulins.

Russian Journal of Immunology. 2019;22(2-1):632-634
pages 632-634 views

CONDITION OF INDICATORS OF INTERACTION OF THE INTER-CELLULAR MATRIX IN MEN WITH CHRONIC CHLAMYDIAL URETHRITIS BEFORE AND AFTER THE TREATMENT

Chepurnova N.S., Kostyushko A.V., Tulupova M.S.

Abstract

The data on the level of matrix metalloproteinase-9 and its tissue inhibitors of the 1st and 2nd types in the serum of venous blood in men with chronic chlamydial urethritis before and after treatment are presented. An increase in the level of MMP-9 against the background of normal values of TIMP-1 and low levels of TIMP-2 indicates the destructive potential of the infectious process. An increase in the effectiveness of combination therapy using recombinant interferon-gamma has been observed.

Russian Journal of Immunology. 2019;22(2-1):635-637
pages 635-637 views

INDICATORS OF FUNCTIONAL ACTIVITY OF NERVOUS AND IMMUNE SYSTEMS IN MICE WITH MUTATIONS IN THE DISC1 GENE

Chizhova N.D., Lipina T.V., Amstislavskaya T.G.

Abstract

Schizophrenia is a multifactorial disease, the pathogenesis of which against the background of immune dysfunction consists of a complex interaction of the genetic characteristics of the organism and environmental factors. To better match the available experimental model of schizophrenia (mouse DISC1- L100P) to the genetic diversity of psychopathology that is observed in humans, we have obtained and studied animals that combine two diff erent mutant alleles of the DISC1 gene (Disrupted-in-Schizophrenia-1). The purpose of this work was to study emotional, social and cognitive behavior in mice carrying two mutations in the DISC1 gene – L100P and Q31L and their comparison to the spectrum of pro- and anti-infl ammatory cytokines in the brain structures of DISC1-L100P mice. Males DISC1-L100P/Q31L mice, in contrast to females, showed a more pronounced defi cit of prepulse inhibition to the 72 dB signal. All studied groups showed the absence of a depressive-like behavior in the tail suspention test. Since DISC1-L100P mice are characterized by changes in the cytokine profi le in the brain structures involved in the pathogenetic mechanisms of schizophrenia, the experimental model of DISC1-L100P/Q31L mice seems promising in studying the contribution of epigenetic eff ects, including maternal immune activation, to schizophrenia pathogenesis.

Russian Journal of Immunology. 2019;22(2-1):638-640
pages 638-640 views

ASSESSMENT OF THE FUNCTIONAL ACTIVITY OF ENDOTHELIUM IN WOMEN WITH HEAVY PREECLAMPSIA DEPENDING MANIFESTATIONS OF THE DISEASE

Chistyakova G.N., Remizovа I.I., Aitov A.E.

Abstract

The functional activity of the endothelium and the level of pro- and anti-inflammatory mediators in women with severe pre-eclampsia (PE) were evaluated depending on the onset of the disease. It was established that the implementation of severe pre-eclampsia regardless of the timing of the manifestation (before or after 34 weeks of gestation) is accompanied by an increase in the level of endothelin-1, TNFα and C-reactive protein. In women with early PE, against the background of the revealed changes, an increase in the level of stable NO2 and IL-10 metabolites is noted, and from late PE, there is a homocysteine content, which makes it possible to use these indicators to assess the severity of preeclampsia with different periods

Russian Journal of Immunology. 2019;22(2-1):641-643
pages 641-643 views

THE IMMUNOREGULATORY AND HEPATOTROPIC ACTION OF GLY-HIS-LYS AND TIMOGEN

Chulanovа A.A., Smakhtin M.Y., Sunaykin K.I., Smakhtina A.M.

Abstract

Gly-His-Lys activated hepatocyte reparative regeneration and decreased immune function in case of liver pathology, as with enhanced immune function (tetrachloromethane hepatopathy), as with decreased immune function (hydrazine hepatopathy). Gly-His-Lys was the most potent hepatocyte mitotic activity activator in comparison with timogen, which also activated reparative liver regeneration and had the immunoregulatory activity.

Russian Journal of Immunology. 2019;22(2-1):644-646
pages 644-646 views

CONTENTS OF IL-18 AND MSR-1 IN CHILDREN WITH CONGENITAL CLEARANCE OF THE UPPER LIP AND SKY

Sharopov S.G., Inoyatov A.S.

Abstract

A study was conducted to study the levels of pro-and anti-inflammatory cytokines (IL-2, IL-6, IL-18, MCP-1 and IL-4) in 46 children with cleft lip and palate, who were in the children’s republican hospital in Bukhara. All sick children underwent a comprehensive examination by specialists (surgeon, pediatrician, orthopedist, otolaryngologist, neuropsychiatrist) to identify abnormalities of a somatic nature and associated developmental defects. Studies on the level of cytokines showed that the synthesis of proinflammatory cytokines ‒ IL-6, IL-18, MCP-1 and anti-inflammatory cytokine IL-10 in children was significantly increased, while the level of IL-2 was slightly lower than the control values. Noted changes in the state of cytokine status can be qualified as a secondary immunodeficiency state, requiring immunomodulatory measures.

Russian Journal of Immunology. 2019;22(2-1):647-649
pages 647-649 views

ANALYSIS OF PHENOTYPIC AND FUNCTIONAL CHARACTERISTICS OF SUPPRESSOR POPULATIONS IN BRONCHIAL ASTHMA

Shevchenko J.A., Kuznetsova M.S., Lopatnikova Y.A., Demina D.V., Sennikova J.A., Vorobyova O.P.

Abstract

In patients with atopic allergic diseases, the number or functional characteristics of T-regulatory cells may be insufficient. The determination of the phenotypic characteristics of T-regulatory cells also makes a significant contribution to such a large variety of data obtained. The main difficulties in finding correlations between suppressor populations and the development of pathogenetic processes: the absence of a well-defined set of markers for T-regulatory cells, dependence on the stage of the disease, the duration of taking glucocorticosteroids and other drugs. No less important in studying the involvement of T-regulatory cells in the development of pathological reactions is not only the analysis of the content of T-regulatory cells, but also the assessment of their functional activity, the level of expression of membrane-bound functional molecules and the production of soluble factors by them.

Russian Journal of Immunology. 2019;22(2-1):650-652
pages 650-652 views

THE INFLUENCE OF IL-7 AND IL-15 ON T-REGULATORY CELLS OF DONORS AND PATIENTS WITH RHEUMATOID ARTHRITIS IN VITRO

Shevyrev D.V., Kozlov V.A., Omelchenko V.O.

Abstract

The aim of this study was to assess the infl uence of homeostatic factors IL-7 and IL-15, that induced homeostatic proliferation (HP), on the phenotypic characteristics of T-regulatory cells (Treg) from healthy donors and patients with rheumatoid arthritis (RA) in vitro. Analysis of Treg phenotype was performed by fl ow cytometry, using Foxp3, CD127, CD4 and CD25. Isolated from the blood cells were cultured with and without stimulation during 7 days. IL-7, IL-15 and anti-CD3 antibodies supplemented IL-2 were chosen as activators. Purifi ed Treg cells were labeled with CFSE dye to assess proliferation. We estimated that HP factors eff ectively maintain the number and phenotype (CD25+FoxP3+) of Treg in vitro. Also, in patients group we showed an increasing of CD4+CD25+FoxP3+ cells under all stimulation condition that may indicate the induction of Treg from conventional T cells. Herewith under IL-7 and IL-15 Treg cells from RA patients had a low proliferative activity than Treg from donors. The reduced proliferative activity of Treg in the group of RA-patients under IL-7 and IL-15 perhaps make a signifi - cant contribution to the development of the disease due to the delay of Treg pool reconstitution in the lymphopenia conditions on the initial stage. Furthermore, increased induction of CD4+CD25+FoxP3+ cells in PBMC cultures in patients group associated with pathogenesis of RA, since induced Treg have transient and unstable expression of FoxP3. 

Russian Journal of Immunology. 2019;22(2-1):653-656
pages 653-656 views

AUTOIMMUNE COMORBID DISORDERS – THE OPPORTUNITIES OF RATIONAL TREATMENT

Shirinsky V.S.

Abstract

In this review of our own data we discuss the problems of the treatment of comorbid conditions using osteoarthritis (OA) and rheumatoid arthritis as examples. We provide a rationale for the use of drugs targeting central molecular nodules which regulate inflammation, immune reactions, lipid homeostasis, and herbal medicines directed on various targets. The results of clinical studies evaluating efficacy, safety and pharmacodynamics of these drugs are presented. The use of fenofibrate and simvastatin in rheumatoid arthritis was effective and well tolerated and was associated with anti-inflammatory and antiatherogenic effects. The use of fenofibrate in patients with erosive OA was associated with the reduction of pain, decreased markers of inflammatoion and had lipid modulating and immune modulating effects. Clinical efficacy and pleiotropic effects of fenofibrate were also observed in other OA phenotype – diabetes-associated OA. The results of clinical trials evaluating herbs Comarum Palustrae and Curcuma longa in OA with metabolic syndrome showed their efficacy, safety and pleotropic effects. The use of drugs targeting molecular hubs and herbal treatments are promising and pathogenetically based approaches to the treatment of autoimmune arthropathies with high risk of comorbidity.

Russian Journal of Immunology. 2019;22(2-1):657-659
pages 657-659 views

THE EVOLUTION OF VIEWS ON THE PATHOGENESIS OF AUTOIMMUNE DISEASES IN THE ERA OF MICROBIOME

Shirinsky I.V.

Abstract

The modern treatment of autoimmune diseases (AIDs) is based on the use of drugs reducing inflammation and inhibiting the immune responses. This strategy leads to the short-term reduction of disease activity but does not change long-term outcomes. Thus, analyses of several large cohorts of patients with rheumatoid arthritis (RA) showed that active use of synthetic and biological disease modifying antirheumatic drugs in the last 20 years did not reduce RA-related disability and all-cause mortality. The discovery of human microbiome allows to reevaluate the nature of autoimmune diseases and suggests that dysbiosis and chronic infection with previously poorly-known/unknown microorganisms are major factor for AIDs development and persistence. In conclusion, in order to develop eff ective treatment of AIDs we need to rethink our general views on the AIDs pathogenesis in the light of new data of human microbiome.

Russian Journal of Immunology. 2019;22(2-1):660-662
pages 660-662 views

PLEIOTROPIC EFFECTS OF DNA METHYLATION MODULATORS ON RHEUMATOID ARTHRITIS SYNOVIAL FIBROBLASTS IN VITRO

Snyder M.A., Kalinovskaya N.Y.

Abstract

To evaluate pleiotropic eff ects of DNA methylation modulators, we used cultures of fibroblast-like synoviocytes (FLS) obtained during total arthroplasty from nine rheumatoid arthritis (RA) patients. It was shown that addition of hydralazine to cultures lead to significant two-fold decrease in of global DNA methylation, whereas addition of donors of methyl group S-adenosyl L-methyonine (SAMe) and genistein resulted in reduction of 5-methylcytosine in FLS by 35%. There was a spontaneous production of IL-6, IL-17, IL-18, IL-10, and osteoprotegerin (OPG) by FLS, the stimulation with IL1β lead to significant increase of these cytokines synthesis. The production of pro-infl ammatory cytokines was reduced only after addition of SAMe to cultures in some doses. Demethylating agent hydralazine had no eff ect on cytokine synthesis, while genistein decreased production of IL-6 and IL-17 in some concentrations. The production of IL-10 was not changed by the modulators of DNA methylation. Addition of SAMe and genistein to cultures lead to significant reduction of OPG production while hydralazine did not have any eff ect on OPG synthesis. All three DNA methylation modulators significantly reduced migration and invasion of FLS in Boyden chambers. In conclusion, cytokine synthesis, migration and invasion of FLS are modulated not only by DNA methylation, but probably via other epigenetic mechanisms. The model of FLS cultures can be used for preclinical assessment of DNA methylation inhibitors.

Russian Journal of Immunology. 2019;22(2-1):663-665
pages 663-665 views

CLINICO-IMMUNOLOGICAL EVALUATION OF THE EFFECTIVENESS OF INTRAVENOUS IMMUNOGLOBULINS AS AN ADJUVANT THERAPY FOR SURGICAL SEPSIS

Shumatov V.B., Lazanovich V.A., Krasnikov V.E.

Abstract

We observed 46 patients with sepsis, which were divided into 2 groups. I group (25 patients) received traditional treatment, II group (21 patients) in addition to conventional therapy received enriched IVIG (IgM+IgA). Comparative assessment of the combination of intravenous immunoglobulin’s and traditional therapy indicated the clinical effectiveness of IVIG therapy, which results in reducing the severity of systemic inflammation, the severity of the disease and the mortality in patients with surgical sepsis.

Russian Journal of Immunology. 2019;22(2-1):666-668
pages 666-668 views

MOLECULAR TARGETS OF THE ACTIONS OF THE INNOVATIVE ANTICONVULSANT META-CHLORO-BENZHYDRYLUREA (M-CL-BHU) IN NEUROIMMUNOLOGY OF DEPENDENCE

Shushpanova T.V., Novozheeva T.P., Perevozchikova T.V., Shushpanova O.V., Knyazeva E.M.

Abstract

Metabolites of the anticonvulsant m-CL-BHU in the process of detoxification on cytochrome P-450 are conjugated with endogenous macromolecules and form a complete stimulus for the immune system. Specific activation occurs at the time of the decrease in the activity of liver microsomal monooxygenases. This protection function is valuable in maintaining “immuno-chemical homeostasis” in the body.

Russian Journal of Immunology. 2019;22(2-1):669-672
pages 669-672 views

EMBRIOGENESIS OF NEURORECEPTOR AND NEUROIMMUNE SYSTEMS OF A HUMAN WITH NEONATAL INFLUENCE OF ALCOHOL ON FETUS

Shushpanova T.V., Solonsky A.V., Kudrenko A.S., Bohan N.A.

Abstract

Alcohol and tissue hypoxia in the early stages of embryonic development has a modifying effect on the formation of the neuroimmune and neurotransmitter systems of the developing brain: microglia, the GABA receptor system, their functional and mediator activity.

Russian Journal of Immunology. 2019;22(2-1):673-676
pages 673-676 views


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