Vol 22, No 2-2 (2019)

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Full Issue

ORIGINAL ARTICLES

INFLUENCE OF THE LEVELS OF THE PRO-INFLAMMATORY CYTOKINES ON THE FORMATION OF EXTRACELLULAR NEUTROPHILIC TRAPS IN DISSEMINATED OVARIAN CANCER

Abakumova T.V., Gening T.P., Dolgova D.R., Antoneeva I.I., Gening S.O.

Abstract

The eff ect of IL-8 and IL-18 o the ability of circulating neutrophils to form extracellular traps (NET), contributing to the proliferation and metastasis of tumor cells, is shown. The aim of the study was to evaluate the eff ect of IL-8 and IL-18 in serum on the formation of NETs in advanced ovarian cancer. We have found that an increase in the levels of pro-infl ammatory cytokines IL-8 and IL-18 in the dynamics of progression of OC is accompanied by a decrease in the number of NETs and an increase in the index of NET formed by circulating neutrophils, which may indicate a decrease in the killer function of the latter.
Russian Journal of Immunology. 2019;22(2-2):704-706
pages 704-706 views

CO-EXPRESSION OF RECEPTORS TO THE ALPHA TUMOR NECROSIS FACTOR ALPHA IN T CELL SUBPOPULATIONS IN RHEUMATOID ARTHRITIS

Alshevskaya A.A., Belomestnova I.A., Zhukova J.V., Chumasova O.A., Shkaruba N.S., Sizikov A.E., Sennikova J.A., Vorobyova O.P.

Abstract

The tumor necrosis factor alpha (TNFα) play key role as the main pro-infl ammatory cytokine in immune processes in rheumatoid arthritis (RA). However, the activity and functions of TNFα can be regulated not only by soluble receptors (which act primarily as neutralizers of action), but also by the amount, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). Analysis of the co-expression parameters of TNFR1 and TNFR2 in RA patients compared with healthy donors showed that changes in the amount and co-expression of type 1 and type 2 receptors for TNF-alpha on the surface of immunocompetent cells are associated with indicators of disease activity.
Russian Journal of Immunology. 2019;22(2-2):707-709
pages 707-709 views

EFFECTS OF PROLINE-RICH PEPTIDES ON THE FUNCTIONAL ACTIVITY OF HUMAN LEUKOCYTES IN VITRO

Artamonov A.Y., Sukhareva M.S., Kopeykin P.M., Sukhachev A.N., Filatenkova T.A., Orlov D.A., Shamova O.V.

Abstract

The effects of peptides with a high content of proline (Proline-Rich Peptides, PRPs) of human saliva (fragments of Basic salivary proline-rich protein 1 – P-H (37–51), P-F (43–61), IB6 (98–116), p1932), as well as of several animal’s neutrophilic PRPs (bactenecins ChBac3.4, ChBac5, mini-ChBac7.5Na), towards cells involved in the functioning of the immune system have been explored. In the presence of each of the investigated PRPs the intensity of the respiratory burst of human blood phagocytes in vitro, challenged by Escherichia coli, was reduced (the effect was more pronounced in the case of salivary PRPs). Administration of ChBac3.4, mini-ChBac7.5Nα, р1932 to human peripheral blood mononuclear cells, stimulated with lipopolysaccharide, caused a decrease of LPS-mediated release of cytokines IL1β, TNFα, without affecting a level of IL10 (for other PRPs the effects were not significant). Thus, the results confirm the anti-inflammatory action of PRPs and point to the suggestion that cationic prolinerich proteins and peptides of saliva may be involved in regulation of inflammation in the oral cavity.
Russian Journal of Immunology. 2019;22(2-2):710-712
pages 710-712 views

Muc2 KNOCKOUT MICE AS A MODEL FOR TESTING APPROACHES TO ALTER GUT MICROBIOTA

Achasova K.M., Litvinova E.A.

Abstract

Gut is inhabited by numerous of bacteria interacting with the immune system of the host. Disturbance of the gut homeostasis leads to various pathological states, which are to be treated by changing of microbiota. The study demonstrate the effects of microbiota alteration on viability of mice with deficient barrier function in the gut (Muc2-/-). The susceptibility of Muc2-/- mice to changing of microbiota suggesting the mice as an appropriate model for testing approaches to alter gut microbiota.
Russian Journal of Immunology. 2019;22(2-2):713-715
pages 713-715 views

ACTIVATION OF INTERFERON SYSTEM GENE TRANSRIPTION BY YEAST DOUBLE-STRANDED RNA

Bateneva A.V., Gamaley S.G., Lebedev L.R., Danilenko E.D.

Abstract

In experiments on mice, the level of expression of antiviral-response-mediating proteins was studied in peritoneal macrophages, in reaction to the intraperitoneal administration of double-stranded RNA (dsRNA) from the yeast Saccharomyces cerevisiae. Dose (0.5 and 1.5 mg/kg) and time (5 and 24 hours) dependencies of the eff ect of dsRNA on the activity of genes encoding Toll-like receptor 3 (TLR3), IFN-alpha, IFN-beta, 2’,5’-oligoadenylatesynthetase (OAS) and proteinkinase R were determined. The obtained results indicate the increase in the level of transcriptional activity in the cells, most pronounced for TLR3, IFN-alpha and OAS genes. The maximum eff ect was observed 5 hours after the administration of dsRNA in the eff ective antiviral dose (0.5 mg / kg).
Russian Journal of Immunology. 2019;22(2-2):716-718
pages 716-718 views

INVESTIGATION OF NEUTROPHIL MIGRATION VIA LYMPHATIC VESSELS IN THE COURSE OF A. FUMIGATUS-INDUCED INFLAMMATION

Bolkhovitina E.L., Fedorina A.S., Bogorodskiy A.O., Smirnova A.O., Chursanova E.N., Sapozhnikov A.M., Borshchevskiy V.I., Shevchenko M.A.

Abstract

Neutrophils are the main eff ector cells that are mediated immune response to exogenous pathogens including opportunistic fungi Aspergillus fumigatus. Neutrophils infi ltrate infl amed tissues and massively die during the fi ght against pathogen. However, in some cases neutrophils survive and migrate to lymphatic vessels to neutralize residual pathogens and activate adaptive immune response. In the present study we characterized the ability of neutrophils to migrate via lymphatic vessels during A. fumigatus conidia-induced infl ammation. As a result, new data about neutrophils migration kinetics to lymphatic vessels and the role of neutrophils in control of fungal spore dissemination in the host organism were obtained.
Russian Journal of Immunology. 2019;22(2-2):719-721
pages 719-721 views

ANALYSIS OF LEVEL EXPRESSION OF THE HUMAN GENE TIM-1 USING REAL-TIME QUANTITATIVE PCR

Bondarenko T.Y., Svyatchenko V.A., Ternovoi V.A.

Abstract

The expression level of a human T-cell immunoglobulin-mucin domain (TIM-1) gene is diagnostically signifi cant in human diseases. The purpose of this work was to develop a real-time PCR method for analysis the expression levels of the human TIM-1 gene. The design of primers and fl uorescent probe on the conservative parts of the TIM-1 gene was carried out. The fragment of the TIM-1 gene was cloned into a plasmid and further it was used as a positive control. The optimization of the PCR conditions was performed using the HEK293 cell culture as a model. The expression levels of the TIM-1 gene were determined in the saliva of ten healthy volunteers and they did not diff er signifi cantly. The system being developed may be an alternative to closed commercial systems. We plan to use it in the research of diff erent pathologies in the future.
Russian Journal of Immunology. 2019;22(2-2):722-724
pages 722-724 views

EXPRESSION OF PLEXIN B1 BY HUMAN T LYMPHOCYTES: ROLE IN THE CONTROL OF CELL ACTIVATION

Valieva Y.V., Kuklina E.M.

Abstract

The paper presents new data on T-lymphocyte expression of plexin B1, a high-affinity receptor for class IV semaphorin Sema4D, which expression was previously attributed exclusively to non-immune cells. The biological significance of this phenomenon, as well as its role in the regulation of T-lymphocyte functions are discussed, primarily of cell migration.
Russian Journal of Immunology. 2019;22(2-2):725-727
pages 725-727 views

GENERATION AND CHARACTERIZATION OF RECOMBINANT ANTIBODY AGAINST THE COMPLEMENT C5a ANAPHYLATOXIN

Vasilishina A.A., Demyanov A.V., Monahova V.S., Zhakhov A.V., Trofimov A.V., Denisenko E.S., Nekrasova K.A., Rodin S.V., Simbirtsev A.S., Ischenko A.M.

Abstract

Monoclonal mouse antibodies were raised against the human C5a anaphylatoxin. In these antibodies, the light- and heavy-chain constant regions were replaced by human IgG constant regions to produce chimeric mouse–human antibodies.
Russian Journal of Immunology. 2019;22(2-2):728-730
pages 728-730 views

CYTOKINE LEVEL IN BLOOD SERUM IN PATIENTS WITH ACQUIRED ANGIOEDEMA

Vlasova A.O.

Abstract

Cytokines are biological markers whose overproduction can lead to an inflammatory reaction and cause the development of pathological reactions. In the present work, the serum levels of IL-17A, IL-17F and IL-13 were studied in 36 patients with acquired angioedema during the period of clinical manifestations. The control group consisted of 10 practically healthy donors. This study showed a high level of IL-17A, IL-17F in patients with acquired angioedema during the period of clinical manifestations, while a comparative analysis showed no differences in the content of serum IL-13 in the control group and the control group. Thus, the determination of the level of IL-17A, IL-17 F in serum can be used as a laboratory diagnostic marker of acquired angioedema.
Russian Journal of Immunology. 2019;22(2-2):731-732
pages 731-732 views

THE ROLE OF DRP1 PROTEIN, RESPONSIBLE FOR MITOCHONDRIAL FRAGMENTATION, IN THE OXIDATIVE BURST AND NETOSIS OF HUMAN NEUTROPHILS

Vorobjeva N.V., Kulakov V.V.

Abstract

Neutrophil mitochondria are in a state of dynamic equilibrium, which is supported by two opposite processes: fusion and fission. Numerous studies have shown that the morphology of mitochondria is closely related to their functional activity. However, it was found that proteins involved in the process of mitochondrial dynamics can modulate other functions of mitochondria too. As was shown in our work on the model of human neutrophils, the dynamin-related protein 1 (DRP1) responsible for mitochondrial fission, is involved in NETosis, realizing its classic function. At the same time, the suppression of the oxidative burst via the DRP1 was due to its non-classical function associated with its action on the mitochondrial pore.
Russian Journal of Immunology. 2019;22(2-2):733-735
pages 733-735 views

IMMUNE DISORDERS AND ARTERIAL PRESSURE PATIENTS WITH ARTERIAL HYPERTENSION

Gavrilyuk E.V., Konoplya A.I., Mikhin V.P., Prokofieva Y.V., Serikova L.N.

Abstract

The study included 68 patients with arterial hypertension 1, 2 and 3 degrees, in whom the concentration of cytokines and components of the complement system was determined in the blood plasma before and after 4 weeks of systemic antihypertensive therapy. In patients with arterial hypertension prior to the onset of systemic antihypertensive therapy, changes in the level of pro-inflammatory and antiinflammatory cytokines and activation of the complement system were found in proportion to the level of blood pressure. In patients with arterial hypertension who have reached the target values of blood pressure against the background of ongoing pharmacotherapy, the severity of immune changes is significantly lower than in patients whose blood pressure has not reached the target level.
Russian Journal of Immunology. 2019;22(2-2):736-738
pages 736-738 views

IMMUNE MODULATING EFFECTS OF MYELOPEPTIDES MP-5 AND MP-6 UNDER THE STRESS CONDITIONS

Gein O.N., Gavrilova T.V., Kadochnikova Y.A., Chereshneva M.V., Chereshnev V.A.

Abstract

The myelopeptides MP-5 and MP-6 are capable of correcting changes in the production of reactive oxygen species and cytokines (IL-1β and IL-10) by peritoneal macrophages under stress.
Russian Journal of Immunology. 2019;22(2-2):739-741
pages 739-741 views

β-ENDORPHIN REGULATION OF SECRETORY ACTIVITY OF PERITONEAL CAVITY CELLS IN MICE UNDER STRESS CONDITION

Gein S.V.

Abstract

It was found that the 2-h immobilization stress reduced the secretion of reactive oxygen species (ROS) and IL-1β by cells of the peritoneal cavity of mice. The introduction to mice of β-endorphin at doses of 100 and 0.0005 μg / kg before immobilization modulated the eff ects of stress.
Russian Journal of Immunology. 2019;22(2-2):742-744
pages 742-744 views

A MONOCHROMATIC INCOHERENT LIGHT OF THE450 NM OPTICAL RANGE AFFECTS THE CONTENT OF CYTOKINES IN THE MAXILLARY WASHES IN RHINOLOGIC PATIENTS

Gizinger O.A., Korkmazov A.M., Korkmazov M.Y., Karandashov V.I., Zavgorodniy E.S.

Abstract

The data on the content of cytokines IL-8, IL-10, IL-1, IL-6 in washes from the mucous membrane of the nasal cavity 24 hours after surgery in patients operated on for the curvature of the nasal septum are presented. The results of the study showed that in rhinosurgical patients on the first day after surgery, an imbalance of cytokines isrecorded, consisting in increasing the amount of IL-8, IL-1α, IL-1β, IL-10, and the content of IL-6 decreases. The use of monochromatic light with a wavelength of 450 ± 10 nm helps to reduce the inflammatory response, edema, restore the balance of cytokines.
Russian Journal of Immunology. 2019;22(2-2):745-747
pages 745-747 views

CHARACTERISTICS OF REMODELLING REGULATORY IMMUNE MECHANISMS IN CARTILAGE AND BONE TISSUES AT INCIPIENT AND LATE PRESENTATIONS OF PRIMARY AND POST-TRAUMATIC OSTEOARTHROSIS

Gladkova E.V.

Abstract

The interrelations of lymphocyte subpopulations pattern in peripheral blood and level of cartilage (Urine CartiLaps (CTX II) and bone (Serum CrossLaps) tissue metabolic markers in biological media exemplifi ed by 74 patients with incipient (0‒I) and late (III‒IV) stages of primary and post-traumatic osteoarthrosis as well as 20 healthy persons aged 45.9±11.4 have been studied. The activation of bone tissue destruction and cartilage extra-cellular matrix disorganization has been discovered at 0-I stages of primary OA, III‒IV stages of primary and post-traumatic osteoarthrosis. The evidences of immunodepression for T-cell immunity have been revealed represented by decrease of (CD3+CD4+) and (CD3+CD8+) lymphocytes. The presence (р<0.05) of mean strength (R = –0.66) negative interrelations between (CD3+CD4+) lymphocytes and Urine CartiLaps as well as (R = –0.70) between (CD3+CD8+) cells and (Serum CrossLaps) at late stages of primary and post-traumatic osteoarthrosis has been proven. In virtue of the obtained results the conclusion about incipient stages of primary osteoarthrosis being characterized with more signifi cant processes of cartilage extra-cellular matrix disorganization and bone tissue destruction has been drawn. III‒IV stages of both primary and post-traumatic osteoarthrosis are characterized with profound loss of type I and II collagen and accumulation of their degradation impurities in biological media accompanied by evidences of cell-immunity depression represented by decrease of T-lymphocytes count due to (CD3+CD4+) and (CD3+CD8+) pools. This proves the generality of pathogenetic remodelling regulatory mechanisms in bone and cartilage skeletal tissues at late presentations of the disease.
Russian Journal of Immunology. 2019;22(2-2):748-750
pages 748-750 views

CONTRIBUTION OF DIFFERENT TYPES OF MELATONINE RECEPTORS TO HORMONE-DEPENDENT REGULATION OF T LYMPHOCYTES PRODUCING IL-17 (TH17) DIFFERENTIATION

Glebezdina N.S., Nekrasova I.V., Olina A.A., Sadykova G.K.

Abstract

The involvement of different types of melatonin receptors in hormone-dependent regulation of the differentiation of T helpers producing IL-17 (Th17) was studied. It has been shown that in low physiological concentrations melatonin realized effects through membrane receptors, whereas the action of the hormone’s pharmacological concentrations was mediated by both membrane and nuclear receptors and was dose dependent.
Russian Journal of Immunology. 2019;22(2-2):751-753
pages 751-753 views

COMPLEMENT IN PATHOLOGIES, POSSIBILITY OF CORRECTION USING A NOVEL HUMANIZED ANTIBODY THAT CAN BLOCK ALTERNATIVE PATHWAY OF COMPLEMENT SYSTEM ACTIVATION

Gorbunov N.P., Zhakhov A.V., Trofimov A.V., Nekrasova K.A., Rodin S.V., Atanesyan E.A., Karabanova E.A., Zakharov M.S., Simbirtsev A.S., Ischenko A.M.

Abstract

Two monoclonal antibodies were developed and were shown to block the complement system alternative pathway (AP) activation at the stage of assembly and activation of C3 convertase. The humanized antibody hC34 specific to a binding site on α’2 determinant of С3с fragment inhibited in vitro the human complement activation by 90% at a dose of 3 μg/ml of blood serum. The antibody 3А8 specific to rat complement C3 component and similar to antibody hC34 was demonstrated to be pharmacologically active in model of closed craniocerebral injury (data of functional and histological studies). The results of this study indicate the humanized antibody hc34 to be promising for treatment of central nervous system pathologies and of other diseases associated with complement hyperactivation.
Russian Journal of Immunology. 2019;22(2-2):754-756
pages 754-756 views

THE STATE OF LOCAL IMMUNITY IN ADOLESCENTS WITH CHRONIC GINGIVITIS

Dzech O.Y., Lazarev A.I.

Abstract

Adolescents with chronic catarrhal gingivitis aged from 14 to 17 years without general somatic pathology affecting the etiopathogenesis of inflammatory periodontal diseases were observed. Of the total number of patients examined, 19 were at the stage of ortho-dontic treatment. The control group consisted of 40 clinically healthy patients. The state of the local immune status of the oral cavity was assessed by the functional activity of phagocytes, the content of mixed saliva IgA, IgM, IgG, sIgA, IL-4, IL-8 by solid-phase enzyme immunoassay. In adolescents with chronic gingivitis, there are clearly pronounced shifts in local immunity, which are represented by an increase in the of IgG, sIgA, IL-4, IL-8, the functional reserve of neutrophils, index stimulation of neutrophils compared to the control group, as well as a decrease in the level of IgM and the concentration of oxygen-dependent enzymes in the cytoplasm of phagocytes in the nbt-spontaneous test.
Russian Journal of Immunology. 2019;22(2-2):757-759
pages 757-759 views

MAST CELLS IN THE SYSTEM OF UTERUS REGULATION IN RATS

Dindyaev S.V., Romashin F.A., Kasatkin D.V.

Abstract

The dynamics of the density of the spatial distribution of mast cells of the body of the womb and the content of catecholamines, serotonin and histamine during sexual cycle, pregnancy and postpartum period in rats were examined by fl uorescence-histochemical methods. The linear correlation of serotonin&catecholamines content in mast cells was kept at high level (during sexual cycle, pregnancy) or was quickly recovered (during postpartum involution) by changing of the uterus functional state. The participation of mast cells in the restoration of uterine sympathetic innervation after birth was considered. The leading role of mast cells in paracrine regulation of uterine tissue morphogenesis was assumed.
Russian Journal of Immunology. 2019;22(2-2):760-762
pages 760-762 views

THREE-LOCUS TLR10–TLR1–TLR6 HAPLOTYPES DISTRIBUTION IN TATARS OF THE CHELYABINSK REGION IN COMPARISON WITH THE POPULATIONS OF EURASIA

Evdokimov A.V.

Abstract

This article describes the cluster TLR10-TLR1-TLR6 haplotypes distribution features observed in Tatars of the Chelyabinsk Region. For the fi rst time in Tatars population the frequencies of three-locus haplotypes – 721A>C in TLR10 gene, 1805T>G in TLR1 gene and 745C>T in TLR6 gene – were obtained. The most similar haplotype frequencies among the world Eurasian populations were identified in Toscans and Iberians, while other Eurasian populations differed significantly from Tatars. The data obtained may be useful in the study of predictors of auto-inflammatory and infectious diseases.
Russian Journal of Immunology. 2019;22(2-2):763-765
pages 763-765 views

INFLUENCE OF 1, 3, 4-TIADIAZINE GROUP COMPOUNDS ON LEUKOCYTE FRACTIONS OF TYPE2 DIABETIC RATS

Emelyanov V.V., Brilliant S.A., Gette I.F., Danilova I.G., Sidorova L.P., Tseitler T.A.

Abstract

The injections of two compounds of the substituted 1, 3, 4-thiadiazines class (L-17 and L-14) were accompanied by normalization of the total number and fractions of peripheral blood leukocytes in rats with type 2 diabetes, obtained by administration of nicotinamide and streptozotocin. L-17 is more effective than L-14 in reducing hyperglycemia.
Russian Journal of Immunology. 2019;22(2-2):766-768
pages 766-768 views

IMBALANCE IN THE CONTENT OF CYTOKINES AND GROWTH FACTORS IN THE INTRAOCULAR FLUID IN PRIMARY OPEN-ANGLE GLAUCOMA

Ermakova O.V., Orlov N.B., Trunov A.N., Konenkov V.I., Chernykh V.V.

Abstract

Was shown increase in the concentrations of cytokines and growth factors (IL-4, IL-6, IL-8, IL-17, TGF-β -1, 2, 3, macrophage infl ammatory protein – 1beta) in the intraocular fluid of patients withdevelopedstage of the primary open-angle glaucoma. This indicates that in the pathogenesis of primary open-angle glaucoma, the activity of the local chronic inflammatory process is determined.
Russian Journal of Immunology. 2019;22(2-2):769-771
pages 769-771 views

THE VARIANTS OF DEATH INNATE IMMUNITY CELLS IN CHRONIC OBSTRUCTIVE LUNG DISEASE OBSTRUCTIVE LUNG

Zinoviev S.V., Plekhova N.G., Kondrashova N.M.

Abstract

A morphological and cytochemical study of the oxidative metabolism of cells bronchoalveolar lavage (BAL) patients with varying degrees of severity chronic obstructive pulmonary disease (COPD) was studed. It was established that along with cell necrosis, enucleation of granulocytes and macrophages is determined, which refers to the morphological feature of programmed death by the formation of extracellular traps – NET-osis. Proven correlation between indicators of netosis, changes in the oxidative metabolism of cells and the degrees of severity of COPD.
Russian Journal of Immunology. 2019;22(2-2):772-774
pages 772-774 views

DEVELOPMENT OF MODELS OF HUMAN MALIGNANT TUMORS FOR CELLULAR IMMUNOTHERAPY

Zlatnik E.Y., Maksimov A.Y., Goncharova A.S., Karnaukhov N.S.

Abstract

Development of ectopic and orthotopic models of human malignant tumors in nude mice with consequent morphologic and immunologic studies was carried out. Morphologic compliancy of tumors having grown in mice to the initial ones was shown. Divergent immunologic changes were found in animals with ectopic and orthotopic tumors.
Russian Journal of Immunology. 2019;22(2-2):775-777
pages 775-777 views

IMMUNOBIOLOGICAL PROPERTIES OF SYNTHETIC ANALOGUES OF GRANULOTSITARNO-MAKROFAGAL KOLONIESTIMULATING FACTOR (GM-CSF)

Zurochka A.V., Zurochka V.A., Dobrynina M.A., Fomina L.O., Zabkov O.I., Gritsenko V.A.

Abstract

It was shown that peptide of the GM-CSF active center is the pluripotent colony-stimulating factor possessing the expressed antibacterial, antiviral, immunostimulating, reparation, radio tire-tread action of in vitro and in vivo. Similar properties the cosmetic preparation ACEGRAM created on its basis possesses.
Russian Journal of Immunology. 2019;22(2-2):778-780
pages 778-780 views

CINETIDINE – CYTOKINES, ANTIBACTERIAL AND ANTIVIRAL ACTIVITY

Zurochka V.A., Zurochka A.V., Fomina L.O., Dobrynina M.A., Zabkov O.I., Gritsenko V.A.

Abstract

Purpose. To analyze the nature of the influence of the synthetic peptide of the active center of granulocyte-macrophage colony stimulating factor (GM-KSF) – ZP2 on the growth in the liquid nutrient medium and biofilm formation of Museum and clinical strains of bacteria, antiviral activity in vitro and in vivo.

Results. It was shown that the peptide of the active center GM-KSF has a pronounced antibacterial effect on a wide range of gram-positive and gram-negative bacteria, reducing the growth of biofilm formation and biomass of bacteria, and the created cosmetic drug ACEGRAM has a similar effect. At the same time sredstva has a strong antiviral dacvim against DNA- and RNA-containing viruses in vitro and in vivo.

Conclusion. Created with synthetic peptide cosmetics Acegram spray and gel have strong antibacterial and antiviral properties in vivo and can be recommended for the treatment of lesions of skin and mucous bacteria and viruses and the peptide that generated the funds is in their mechanisms of action and properties of classical kinocidinum. 

Russian Journal of Immunology. 2019;22(2-2):781-783
pages 781-783 views

STING ACTIVATION IN T CELLS LEADS TO UPR AND CELL DEATH

Ilyukha V.V., Larkin B., Prohorova A.P., Poltorak A.N.

Abstract

Despite well-studied role of the adapter STING in the cell response to cytosolic DNA, its contribution to the activation of programmed cell death was not considered at the proper level. In this study was shown that activation of STING in T cells leads to production of typeI interferons, upregulation expression levels of the interferon-stimulating gene group (ISG), as well as cell death. It is also shown that cell death is mediated by UPR (Unfolded Protein Response).
Russian Journal of Immunology. 2019;22(2-2):784-785
pages 784-785 views

THE FEATURES OF IL-4 AND IL-10 PRODUCTION IN UPPER GASTROINTESTINAL INFLAMMATORY DISEASES

Iraklionova N.S., Belan E.B., Turkina S.V., Rudobaba E.L.

Abstract

The peculiarities of the IL-4 and IL-10 production have been studied in patients with upper gastrointestinal inflammatory diseases. It has been revealed that the serum level of these cytokines depends on presence or absence of allergic diseases and H.pyloriinfection.
Russian Journal of Immunology. 2019;22(2-2):786-788
pages 786-788 views

THE ROLE OF NGAL FOR PREDICTING RENAL DAMAGE IN PEDIATRIC PRACTICE

Kalmetyeva L.R., Ibrahimova L.G., Prolygina D.D.

Abstract

As it is known, serum creatinine and glomerular filtration rate are considered to be markers of kidney damage. In chronic kidney disease, these markers reveal a late stage of kidney damage, when drug therapy is not always effective and the process of kidney damage is irreversible. In recent years, special attention has been paid to the search for predictors of acute and relapses of chronic renal damage, which could lead to the detection of premorbid pathological changes in the kidneys, to determine the nature and differentiate the defeat of various parts of the nephron, to establish the stage of the process, to assess the severity of inflammation and the intensity of fi brogenesis. One of these early markers is NGAL are investigated.
Russian Journal of Immunology. 2019;22(2-2):789-791
pages 789-791 views

THE EFFECT OF CHICKEN YOLK IMMUNOGLOBULIN Y ON INCREASE IN EDURANCE OF MICE

Kaplin V.S., Sysoeva G.M., Gridunova L.V., Kuleshova N.I., Lebedev L.R.

Abstract

The evaluation of adaptogenic properties of lyophilized immunoglobulins Y isolated from chicken yolks was performed with biological testing methods (IgY). In animal experiments, in the forced swimming test (FST), a prior five- and ten-fold oral administration of yolk chicken IgY in a dose of 2 mg per mouse led to a relevant increase in animal endurance.
Russian Journal of Immunology. 2019;22(2-2):792-794
pages 792-794 views

PROPERTIES OF HUMAN NATURAL KILLER CELL SUBPOPULATIONS EXPRESSING MARKERS OF ADAPTIVE CELLS

Kobyzeva P.A., Streltsova M.A., Erokhina S.A., Kovalenko E.I.

Abstract

Natural killer cells are attributed to lymphocytes of innate immunity for their ability of spontaneous antitumor activity without prior activation. However, recent studies have shown that some viral infections, such as human cytomegalovirus, can cause an increase in the number of NK cells with the phenotype CD57+NKG2C+ compared to healthy donors. It is assumed that cells of this subpopulation have some properties of adaptivity and immunological memory to HCMV infection. This paper is devoted to the study of properties of human NK-cell subpopulations selected for the markers CD57 and NKG2C, when stimulated with IL-2 and membrane-bound IL-21.
Russian Journal of Immunology. 2019;22(2-2):795-797
pages 795-797 views

MICROBICIDAL EFFECT OF SECRETORY PRODUCTS OF NEUTROPHILS IN RELATION TO REPRESENTATIVES OF RESIDENT AND CONDITIONALY PATHOGENIC MICROFLORA OF A HUMAN BODY

Kolbina E.V., Shishkova Y.S., Mezentseva E.A., Kolbin I.A.

Abstract

A study of 50 samples of intact and activated neutrophil supernatants from S. aureus (strain 209), E. сoli (strain M-17), Lactobacterium spp., Bifi dobacterium spp. has been conducted. It has been determined that in vitro induction of neutrophils by representatives of the resident and conditionally pathogenic microflora of the human body leads to secretion of biologically active products of different composition. Thus, the interaction of neutrophils with Lactobacterium spp. and, to a greater extent, Bifidobacterium spp. significantly increases the level of nitric oxide in supernatants, while the content of peroxidase and catalase does not change. This is accompanied by a significant increase in the microbicide of neutrophil secretion products in relation to S. aureus and E. coli, without affecting the viability of Lactobacterium spp. and Bifidobacterium spp.
Russian Journal of Immunology. 2019;22(2-2):798-800
pages 798-800 views

THE STUDY OF THE FUNCTIONAL CHARACTERISTICS OF NEUTROPHILIC GRANULOCYTES WHEN EXPOSED TO IRON-CONTAINING NANOPARTICLES FERRIHYDRITE

Kolenchukova O.A., Stolyar S.V., Ladynina L.P.

Abstract

The study is devoted to determining the effect of magnetic iron-containing nanoparticles on the functional state of blood neutrophils. The object of the study was neutrophil granulocytes (n=34). 3 groups were formed: control (without incubation); in the second group – exposed to synthetic magnetic ironcontaining ferrihydrite nanoparticles; in the third group – synthetic magnetic iron-containing ferrihydrite nanoparticles, in the third group – synthetic magnetic iron-containing ferrihydrite nanoparticles coated with natural polysaccharide – arabinogalactane. The concentration of nanoparticles ferrihydrite was 5 mg/ml and 20 mg/ml. Explored the early and late chemiluminescent response. It was found that the functional activity of cells under the influence of different experimental types of iron-containing nanoparticles varies depending on the concentration, with an increase in the number of acting nanoparticles, a unidirectional reaction with a decrease in cell activity is observed.
Russian Journal of Immunology. 2019;22(2-2):801-803
pages 801-803 views

BIOLUMINESCENT CHEMILUMINESCENT MARKERS FOR ASSESSMENT OF PHYSICAL STATUS OF ATHLETES FOR DETERMINATION PERFORMANCE LEVEL

Kolenchukova O.A., Gvozdev I.I., Birukova E.N., Sutormin O.S., Stepanova L.V., Smirnova S.V.

Abstract

The aim of this study was to determine the activity bioluminescent chemiluminescent markers of saliva, serum and blood phagocytes as markers of the physical status of athletes. 2 groups of athletes, with the prevailing aerobic (skiers and ice-skaters, n=16) and anaerobic (sliders and bobsled racers, n=16) types of exercise, aged from 16 to 30 years old, were selected. Studies were carried out under the PWC170 testing. Saliva and blood samples were collected before and under W1, W2, PWC170 types of loads. The determination of activity of pro- and antioxidants was registered using the chemiluminescent method. The determination of the physiologic status was registered using the bioluminescent method. The imbalance in the activity of pro-oxy- and antioxidants was detected for athletes with prevailing aerobic loads. For these athletes were measured increased production of pro-oxidants and decreased the production of antioxidants. In addition, irreversible changes, inhibition of the activity of antioxidant mechanisms, and increased production of reactive oxygen intermediates were received. The opposite situation is observed in athletes with a predominant anaerobic load. Results of bioluminescent bioassay show signifi cant differences between athletes with prevailing aerobic and anaerobic types of loads, as well as with diff erent sports qualifications.
Russian Journal of Immunology. 2019;22(2-2):804-806
pages 804-806 views

THE MODEL OF PSORIASIFORM DERMATITIS IN MICE INDUCED BY INTRADERMAL ADMINISTRATION OF RECOMBINANT HUMAN IL-36γ

Kolobov A.A., Sazonova T.A., Alexandrov G.V., Petrov A.V.

Abstract

We developed and characterized the model of psoriasiform dermatitis in mice induced by induced by intradermal administration of recombinant human IL-36γ. Induced skin lesions resemble human psoriasis in means of both macroscopic (PASI) and microscopic features (acanthosis, parakeratosis, dermal leucocyte infiltration). This model can be used for anti-psoriatic drugs efficiency research.
Russian Journal of Immunology. 2019;22(2-2):807-809
pages 807-809 views

IMMUNE DISORDERS IN PATIENTS WITH COMMUNITYACQUIRED PNEUMONIA

Konoplya E.N., Polyakov D.V., Losenok S.A.

Abstract

Community-acquired pneumonia remains one of the most common infectious diseases. Changing epidemiological situation makes it necessary to revise the existing approaches to its treatment.Immune and metabolic disorders are studied before and after standard medical therapy in patients with community-acquired pneumonia. The patients included in the research had laboratory criteria which indicated presence of immune inflammation, oxidative stress, endothelial dysfunction and activation of lipid peroxidation. The performed standard treatment does not regulate the majority of modified parameters of immune and metabolic status, what stipulates the necessity of search for the methods of correction of disorders.
Russian Journal of Immunology. 2019;22(2-2):810-812
pages 810-812 views

Th2 TYPE CYTOKINES SYNTHESIS AND PRODUCTION AT SYSTEMIC AND LOCAL LEVEL IN PATIENTS WITH INFERTILITY AND ENDOMETRIOSIS

Krasilnikova A.K., Makyshkina A.I., Sotnikova N.Y., Antziferova Y.S.

Abstract

It has been established that during endometriosis, regardless of the severity of the disease, at the systemiс level IL-4 synthesis, intracellular expression of IL-4 and IL-6 were increased in the total lymphocyte and in the T-helper population, and in the peritoneal fluid the level of IL-6+ lymphocytes was increased. Changes in the synthesis, intracellular expression and the serum IL-4 content were proportional to the severity of endometriosis. In endometriosis stages I–II the intracellular production of IL-5 increased in the total lymphocyte population and in the T-helper pool. In endometriosis III–IV the IL-6 synthesis by peripheral lymphocytes increased, as well as the serum level of IL-4, the amount of IL-4+, CD4+IL-4+ lymphocytes and sCD30 in the peritoneal fluid. 

Russian Journal of Immunology. 2019;22(2-2):813-815
pages 813-815 views

PREVALENCE OF ANTINUCLEAR ANTIBODIES IN HEALTHY CHILDREN AND ADULTS

Krivolapova I.M., Pashnina I.A., Chereshnev V.A.

Abstract

Healthy donors (n=100) of 21–59 years old and healthy children (n=55) of 2–17 years old were examined. Antinuclear antibodies were determined by indirect immunofluorescence assay. It was found that 7 adults (7%) and 10 children (18%) had diagnostic titers of antinuclear antibodies detected by indirect immunofluorescence assay. In healthy children and adults homogeneous and speckled fluorescence patterns were observed more often.
Russian Journal of Immunology. 2019;22(2-2):816-818
pages 816-818 views

CYTOKINES IN THE PATHOGENESIS OF SPONTANEOUS URTICARIA

Krivonkina A.E., Yudina S.M., Bahirev A.M.

Abstract

IL-4 and TNFα cytokines are involved in the pathogenesis of spontaneous urticaria and can be considered as potential biomarkers of the disease.
Russian Journal of Immunology. 2019;22(2-2):819-821
pages 819-821 views

REACTION OF CELLULAR FACTORS IN INNATE AND ADAPTIVE IMMUNITY OF THE PIGLETS ON THE INTRODUCTION OF THE DRUG «ANANDIN»

Кryachko O.V., Khomenko R.M.

Abstract

The purpose of this study is to study the eff ect of the drug “Anandin” on some cellular factors of innate and adaptive immunity in piglets. “Anandin” is a drug from the group of immunomodulators that contains two types of compounds, these are derivatives of acridone acetic acid, as well as nitrogencontaining a-D-glucofurase. For the experiment, 30 piglets were selected 15 days before weaning (40 days old). The mass and physiological state of the animals was the same. The study involved a single administration of 10% solution “Anandin” experimental group of animals at a dose of 15 mg/kg body weight. The control group was intact. Before and after the introduction of the drug were carried out laboratory blood tests. The results showed that “Anandin” has a positive eff ect on the performance of cellular factors of immunity. The parameters studied: the activity of oxygen-dependent and oxygen-independent bactericidal systems of neutrophils (LKT and NBT-stimulated), production of lymphokines in RTML, removal of antigens (level of circulating immune complexes).
Russian Journal of Immunology. 2019;22(2-2):822-824
pages 822-824 views

FUNCTIONAL ACTIVITY OF NEUTROPHILS OF BLOOD OF RABBITS WITH SINGLE AND COMBINED STRESS IMPACT

Kryachko O.V., Lukoyanova L.A., Taran A.M.

Abstract

The purpose of this study is to study the effect of single and combined stress effects on the neutrophil function of the blood of rabbits in the experiment. For the experiment used 10 male rabbits, age ‒ 8 months, live weight 3,40±0,73 kg. In the first series of experiments, transport stress was modeled by transporting animals in a car. We caused immobilization stress in the second series of experiments by fixing the rabbit with its legs in the supine position. In the third series of experiments, a combination of effects was performed (immobilization, then, after three days, transportation). Phagocytic activity of blood neutrophils, phagocytic index, digestion index (killing) were determined. It was shown that the combination of stress effects negatively affects not only the phagocytic activity of neutrophils directly during their layering, but also the restoration of the functional systems of blood phagocytes in the post-stress period compared to single stresses.
Russian Journal of Immunology. 2019;22(2-2):825-827
pages 825-827 views

CHANGES IN THE INDICES OF SYSTEMIC IMMUNITY DURING COSMETIC PROCEDURES AFFECTING THE QUALITY OF THE SKIN

Kudrevich Y.V., Kuznetsova E.K., Ziganshin O.R.

Abstract

In the article changes in the indices of systemic immunity before and after cosmetic procedures are considered, the comparative characteristic of changes in the number of cells and their functional capabilities for various types of cosmetology procedures, such as laser exposure, biorevitalization with monotherapy and with their complex action, сhanges in the quantitative content of different clusters of immune cells and their phagocytic activity were noted. An evaluation of the changes in immunoglobulins in the blood before and after cosmetic procedures aimed at improving the quality of the skin.
Russian Journal of Immunology. 2019;22(2-2):828-830
pages 828-830 views

COMPARATIVE CHARACTERISTICS OF THE INDICES OF SYSTEMIC IMMUNITY AND SKIN IMMUNITY BEFORE AND AFTER THE PROCEDURE OF BIOREVITALIZATION

Kudrevich Y.V., Kuznetsova E.K., Ziganshin O.R., Sychugov G.V., Zayats T.A., Emelina A.I.

Abstract

The study involved healthy women, the average age of whom was 45.0±3.41. All patients received a single procedure for the biorevitalization of the facial skin. Some indicators of systemic and tissue immunity were evaluated. When analyzing the number of immune cells in the skin before and after the procedure, the amount of CD3+, CD4+, CD8+ was doubled. The amount of CD20+ remained practically unchanged. When assessing immunoglobulins M, G in the skin before and after the procedure, a decrease in these indicators was observed. IgA practically did not change. The amount of immunoglobulins in the skin changed as well as in the blood. It decreased. When assessing the parameters of cellular immunity of blood, it was found out that the cellular composition of the blood does not practically change. Significant changes occurred only in the absolute and relative amount of CD3CD56+. These indicators signifi cantly increased.  

Russian Journal of Immunology. 2019;22(2-2):831-833
pages 831-833 views

INFLUENCE OF GLUTAMYL-TRYPTOPHAN ON CYTOKINE SECRETION, WOUND HEALING, AND ANTIOXIDANT SYSTEM IN VITRO AND IN VIVO

Kudriavtceva T.A., Starikova E.A., Voronkina I.V., Smirnov V.S.

Abstract

Dipeptide glutamyl-tryptophan influences on metabolic processes in different cells, stimulates the functional activity of immune cells, restores disorders in the antioxidant system, improves tissue regeneration, accelerates wound healing, activates the functions of connective tissue cells, endotheliocytes, macrophages and leukocytes in the outbreak damage.
Russian Journal of Immunology. 2019;22(2-2):834-836
pages 834-836 views

PHENOTYPING OF ANTIGEN-SPECIFIC CYTOTOXIC T-LYMPHOCYTES BY THE METHOD OF MULTICOLOR FLOW CYTOMETRY

Kuznetsova M.S., Lopatnikova J.A., Sennikov S.V.

Abstract

Modern methods of multicolor flow cytometry in combination with MHC multimer technologies allow not only to identify and isolate cytotoxic T-lymphocyte (CTL) populations, but also to conduct phenotypic research on the level of differentiation, functional features of these cells, and even to investigate the distribution of memory T-cell subsets inside populations specific to particular epitopes of tumor antigens. In the present study, we assessed the level of differentiation and the content of memory T-cell subpopulations in cytotoxic T-lymphocyte populations specific for HER2/neu antigen epitopes (HER2) using the MHC-multimers antigen-specific CD8+ T-cell staining method and multicolor flow cytometry. The resulting HER2-specific T cells to a large extent (about 40‒50%) have the TSCM phenotype, a population capable of the most pronounced antitumor immune response due to a combination of effector properties with self-sustainability. 

Russian Journal of Immunology. 2019;22(2-2):837-839
pages 837-839 views

RECEPTOR FUNCTIONS OF SEMA4D IN THE CONTROL OF T-LYMPHOCYTE PROLIFERATION

Kuklina E.M.

Abstract

Сlass IV semaphoring Sema4D is widely represented in the immune system, both in membrane and in soluble form, and controls immune processes, realizing its effects through specific receptors ‒ these are generally accepted ideas. This paper presents data indicating the presence of an alternative way of Sema4D involvingin immunoregulation: this way suggests the functioning of membrane semaphorin as a receptor, which conducts a signal into the cell it is expressed on. Possible signaling mechanisms from membrane Sema4D are discussed.
Russian Journal of Immunology. 2019;22(2-2):840-842
pages 840-842 views

EFFECTS OF BAY 11-7082 ON THE FORMATION OF IMMUNOSUPRESSOR DENDRITIC CELLS IN VITRO

Kurilin V.V., Khantakova Y.N., Tereshchenko V.P., Kulikova E.V., Melentiev V.S., Obleukhova I.A.

Abstract

Dendritic cells (DCs) regulate immune responses by modulating T and B cells in the direction of effector or tolerogenic responses. In this study, the effect of BAY 11-7082 on the formation of immunosuppressive DC of C57Bl/6 mice in vitro using recombinant IL-10 or TGF-β was evaluated. It was shown that the addition of BAY 11-7082 to the culture of immature DCs did not significantly affect the expression of the maturation molecules of dendritic cells (CD80, CD86). When using BAY 11-7082 in combination with IL-10 or TGF-β, reduced the expression of these molecules. The joint cultivation of DC with splenocytes in the presence of BAY 11-7082 and IL-10/TGF-β promoted the differentiation of regulatory T-cells (CD4+CD25hiFoxP3+) and did not contribute to the differentiation of CD4+ T-cells producing IL-10.
Russian Journal of Immunology. 2019;22(2-2):843-845
pages 843-845 views

COMPARATIVE CHARACTERISTICS OF THE SUBPOPULATION COMPOSITION OF LYMPHOCYTES AND HEAT SHOCK PROTEIN IN PATIENTS WITH VIBRATION DISEASE

Kurchevenko S.I., Bodienkova G.M., Lakhman O.L.

Abstract

Comparative analysis of the subpopulations of lymphocytes in patients with vibration disease (VB) from the effects of local vibration revealed the features of the immune response, characterized by increasing the absolute amount of CD3+-, CD4+-, CD8+- lymphocytes and decreased the percentage of CD20+-, CD25+-lymphocytes. In patients with VB from the effects of combined vibration, an increase in the absolute number of CD3+-, CD4+ -, CD8+ -, CD16+ -, CD20+-, CD25+-, CD95+-lymphocytes was observed, which indicates the activation of lymphocyte apoptosis under conditions of pronounced stimulation of the immune system. A decrease in the level of HSP70 in the serum of patients with VB from exposure to local vibration indicates its accumulation inside the cell and the protection of the processes of biosynthesis and the structural integrity of proteins in the damaged cell due to B-lymphocytes. 

Russian Journal of Immunology. 2019;22(2-2):846-848
pages 846-848 views

THE ROLE OF MACROPHAGES IN REGENERATION OF MUSCLE TISSUE INDUCED BY ALLOGENIC BIOMATERIAL

Lebedeva A.I., Muslimov S.A., Afanasiev S.A., Kondratieva D.S.

Abstract

Allogeneic biomaterials (AB) after implantation in a defect in the calf muscles of rats and the uterine horn of the rat contribute to the regeneration of tissues due to migration of M1 macrophages expressing Pro-inflammatory factors, TNFα, IL-1. They inhibit the migration of M2 macrophages, fibrosis factors, promote migration and differentiation of myocytes. IN ischemic damaged myocardium after AB implantation, on the contrary, cd68 macrophage deficiency was found, but high expression by Timp2 cells compared to the control, which could contribute to the reduction of scar area. After the introduction of AB in muscle tissues revealed resident macrophages with phenotype IL-1+/CD68+/PCNA+/Hale+/ vimentin+/FGF1- /TGF-β1

Russian Journal of Immunology. 2019;22(2-2):849-851
pages 849-851 views

EXPERIMENTAL MODEL FOR THE STUDY OF A INDEPENDENT FROM THE LYMPHOCYTES OF POLARIZATION INTESTINAL MACROPHAGES IN THE MICE

Litvinova E.A., Achasova K.M.

Abstract

Acute inflammatory begins to activate of M1-macrophage by classical pathway. In the chronic form of the disease, the body triggers an adaptive alternative pathway to activate of M2-macrophages. For example, the number of intestinal M2-macrophages increases in infl ammatory bowel disease (IBD) of a chronic form. We have got a model of mice that do not have mature lymphocytes and these animals development of IBD due to the absence of a intestinal mucosal barrier. We obtained a good model that reveals the role of macrophages and lymphocytes in the development of IBD, and can also be used to understand the effect of pharmacological drugs that cause polarization of macrophages.
Russian Journal of Immunology. 2019;22(2-2):852-854
pages 852-854 views

STEARYLAMINE ACTIVATES RAPID AND ROS-INDEPENDENT FORMATION OF NEUTROPHIL EXTRACELLULAR TRAPS (NETOSIS)

Lotosh N.Y., Selischeva A.A., Vorobjeva N.V.

Abstract

Using confocal fluorescence microscopy it was shown that stearylamine (SA) at concentration of 0.2 mg/ml causes the neutrophil extracellular traps formation. In comparison with classic NET activator, the phorbol ether of myristate acetate (PMA), SA activates this process much faster, in 30‒60 min, while under PMA stimulation it occurs in 2‒3 hours. Using luminol-dependent chemiluminescence, we showed that SA does not activate NADPH oxidase. SA dose-dependently suppresses the oxidative burst stimulated with PMA, zymosan, or latex. The direct evidence that NADPH oxidase is not activated under the SA action was obtained by using neutrophils isolated from the blood of patients with chronic granulomatous disease (CGD). It is obvious from our results, that SA-induced NET formation does not require the participation of reactive oxygen species and proceeds according to a nonclassical mechanism.
Russian Journal of Immunology. 2019;22(2-2):855-857
pages 855-857 views

SYNERGISTIC INTERACTIONS OF NOD1 AND TLR4 RECEPTORS OF INNATE IMMUNITY

Murugina N.E., Budikhina A.S., Maximchik P.V., Dagil Y.A., Balyasova L.S., Murugin V.V., Chkadua G.Z., Pinegin B.V., Pashenkov M.V.

Abstract

We performed a simultaneous analysis of cytokine expression and metabolic reprogramming of macrophages upon combined stimulation of NOD1 and TLR4 receptors of innate immunity. NOD1 and TLR4 agonists boosted main parameters of glycolysis (extracellular acidification rate, glucose consumption, lactate release). However, changes of these parameters upon combined stimulation were not greater than those induced by stimulation of each individual receptor. At the same time, combined stimulation synergistically induced pro-inflammatory cytokine production and mRNA expression at relatively late time points (4–9 hours) after addition of agonists. In all, metabolic reprogramming may support synergistic induction of cytokines upon combined NOD1 and TLR4 stimulation; however, the origin of this synergy is in the synergistic induction of cytokine gene expression.
Russian Journal of Immunology. 2019;22(2-2):858-860
pages 858-860 views

THE STATE OF CONNECTIVE TISSUE ELEMENTS OF THE LIVER WITH DIFFUSE TOXIC DAMAGE AND ITS CORRECTION

Medvedeva S.Y., Shafigullina Z.A., Danilova I.G.

Abstract

An experimental model of diffuse toxic rat liver damage with carbon tetrachloride (CCl4) and its correction by aminophthalhydrazide (APH) was used to evaluate the response of mast cells and the degree of fibrosis. Structural disorders and suppression of regenerative processes in the liver under conditions of chemical trauma were accompanied by increased migration of mast cells to the portal stroma and an increased content of secretory granules in them. The use of APH contributes to the normalization of the area, occupied by collagen in the liver tissue, and reduces the number of mast cells, but maintains a high content of secretory granules in them.
Russian Journal of Immunology. 2019;22(2-2):861-863
pages 861-863 views

SOME FEATURES OF MAST CELL REACTION ON CHRONIC LOCAL INFLAMMATION

Mukhlinina E.A., Yakovleva O.V.

Abstract

Chronic productive inflammation causes the development of general regenerative response, in which mast cells are directly involved. Staging of their reaction is consistent with the classical inflammation development pattern. The processes of mediators’ accumulation and the replenishment of the mast cell tissue pool replace the initial secretion activation.
Russian Journal of Immunology. 2019;22(2-2):864-866
pages 864-866 views

INFLUENCY OF INFLUENCES OF REGULATORY PEPTIDES AND RECOMBINANT IFNα ON THE PHENOTYPE OF THE SUBSET CD66b+CD33+CD16+CD11b+NEUTROFIL GRANULOCYTES IN VITRO

Nesterova I.V., Chudilova G.A., Kovaleva S.V., Lomtatidze L.V., Rusinova T.V., Tarakanov V.A., Barova N.K., Pavlenko V.N.

Abstract

A model of the bacterial process under the influence of fMLP was reproduced in the in vitro system, which made it possible to reveal the features of the transformation of CD66b+CD33+CD16+CD11b+neutrofi l granulocytes (NG). Multidirectional effects of mono-effects of peptides and rIFNα2b on the expression of the studied markers of NG in healthy children were identified. The modulating effect of rIFNα2b on the transformed fMLP phenotype of NG is associated with polarization into an anti-inflammatory phenotype. 

Russian Journal of Immunology. 2019;22(2-2):867-869
pages 867-869 views

THE INFLUENCE OF IMMUNOLOGICAL REACTIVITY TO THE MYCOBACTERIAL INFECTION PROCESS IN PATIENTS WITH PULMONARY TUBERCULOSIS

Nikitina I.Y., Serduk Y.V., Nenasheva T.A., Shorochova V.A., Bagdasarian T.R., Lyadova I.V.

Abstract

The ratio between the inflammatory and regulatory cytokines involved in immune response during infection M. tuberculosis (Mtb) determines the course of pulmonary tuberculosis (TB). We analyzed the production levels of 44 cytokines associated with innate and adaptive immunity by multiplex analysis in antigen-induced plasma (Ag-plasma) and serum of TB patients (TBP) and healthy donors who had contacted with TBP («TBC»). In TBP, the production levels of IL-2, IFN-γ, IP-10 in Ag-plasma were higher than in TBC (p<0.005). TBP with initially increased production levels of 12 cytokines in Ag-plasma were characterized by severe TB and slow responsiveness to anti-TB therapy compared to patients with initially low production levels of these cytokines. In conclusion, the production levels of IL-2, IFN-γ, and IP-10 were increased in patients with active TB. The immune reactivity evaluation of TBP with recently diagnosed TB can predict the TB severity and patients’ responsiveness to anti-TB therapy.
Russian Journal of Immunology. 2019;22(2-2):870-872
pages 870-872 views

SILK-BASED BIOMATERIALS FOR MODULATION OF IMMUNE RESPONSE

Nosenko M.A., Arkhipova A.Y., Atretkhany K.N., Drutskaya M.S., Nedospasov S.A., Moisenovich M.M.

Abstract

Biomaterials have recently been recognized for their potential use in regenerative medicine and tissue engineering, since they not only represent a scaff old for cell populations, but also can infl uence functionality of diff erent cell types. In particular silk-based biomaterials (i. e. fibroin or spidroin) are of high interest, because they show prominent mechanistic features and have proregenerative potential. In our work we showed that composite fibroin/gelatin scaff olds induce moderate infl ammation both in vitro and in vivo and this eff ect depends on the configuration of the matrices. In particular, culturing of Mouse Embryonic Fibroblasts (MEF) on three-dimensional (3D), but not two-dimensional (2D) fibroin/gelatin scaff olds resulted in overexpression of adhesion molecules ICAM-1 and VCAM-1 as well as proinfl ammatory cytokines TNF and IL-6. Moreover subcutaneous injection of fibroin/gelatin microparticles in mice lead to accumulation of infl ammatory myeloid cells and acceleration of skin wound healing, while implantation of 3D fibroin/gelatin scaff olds under the kidney capsule promoted lymphocytes clusterization, resulting in generation of primitive lymphoid tissue. Altogether our data suggest immunomodulatory features of fibroin/gelatin scaff olds, which can be useful in tissue engineering and regenerative medicine.
Russian Journal of Immunology. 2019;22(2-2):873-875
pages 873-875 views

TUMOR NECROSIS FACTOR IN REGENERATION OF DEEP SKIN WOUNDS IN MICE

Nosenko M.A., Ambaryan S.G., Nedospasov S.A., Drutskaya M.S.

Abstract

Mechanisms of skin wound healing have been extensively studied in order to propose novel approaches for treatment of chronic and poorly healing wounds, including those associated with autoimmune pathologies, as well as for fibrosis prevention. Recent studies indicate the key role of proinfl ammatory cytokines, such as TNF, IL-6 and IL-1, in regulating skin regeneration. In our work we investigated the impact of tumor necrosis factor (TNF) on healing of deep skin wounds in mice by employing reverse genetics approach. We found, that conditional knock-out of TNF gene specifically in macrophages results in delayed wound healing. However inactivation of TNF in all cell types does not aff ect wound regeneration dynamics. Moreover, genetic knock-out of TNF receptor I (TNFRI) results in accelerated wound healing. Thus, the results of our study suggest that TNF from diff erent cell types plays a dual role in skin regeneration probably refl ecting that both cellular source of TNF as well as receptor type on target cells are important.
Russian Journal of Immunology. 2019;22(2-2):876-878
pages 876-878 views

CLINICAL AND IMMUNOLOGICAL EVALUATION OF THE EFFECT OF USING MILIACIL AND EHF-THERAPY IN THE TREATMENT OF PURULENT WOUNDS IN PATIENTS WITH DIABETES

Nuzova O.B., Studenikin A.V., Filippova Y.V., Stadnikov A.A.

Abstract

The purpose of the study is to evaluate the eff ectiveness of purulent wounds treatment with the local combined use of Miliacile and EHF-therapy in patients with diabetes mellitus, based on determining the cytokine content. In 35 patients of the main group, Miliacile and EHF therapy were used in the local treatment of wounds, while in 35 patients of the control group, only Miliacile was used and the levels of TNFα, IFNγ, IL-4, IL-10 were determined in the blood serum of these patients upon admission to hospital, on the 7th day and at the end of treatment. As a result of treatment, there was observed a more pronounced increase in the level of cytokines (IFNγ, IL-4) and their decrease (IL-10, TNFα) in the patients of the main group as compared with patients of the control group. The eff ectiveness of purulent wounds treatment in patients with diabetes mellitus with the local use of Miliacile and EHF-therapy based on the determination of cytokine levels was determined.
Russian Journal of Immunology. 2019;22(2-2):879-881
pages 879-881 views

INFERTILITY OF PIPE GENESIS: IMMUNE AND METABOLIC DISORDERS AT THE SYSTEM AND LOCAL LEVEL

Omasharifa Po B.G., Konoplya A.A.

Abstract

The study included patients aged 18‒40 years with a burdened obstetric and gynecological history (chronic pelvic infl ammatory diseases, history of infertility, spontaneous abortions, miscarriage, abortions). In patients with tubal infertility, there is a violation of a number of indicators of immune status, both at the system level and at the local level, «oxidative stress» develops, which is manifested in the violation of lipid peroxidation processes. The revealed violations of the immune and metabolic statuses are not fully corrected by the standard treatment regimen, which requires the inclusion of additional immunotropic drugs in the treatment regimen of these patients.
Russian Journal of Immunology. 2019;22(2-2):882-884
pages 882-884 views

THE INFLUENCE OF VITAMIN D3 AS PART OF A NEW DOSAGE FORM FOR IgG, IgM, IL-8 IN BLOOD SERUM IN EXPERIMENTAL ULCERATIVE COLITIS

Osikov M.V., Simonyan E.V., Boyko M.S.

Abstract

The use of anti-inflammatory drugs for the basic therapy of ulcerative colitis (UC) (5-aminosalicylic acid (5-ASA), glucocorticoids) is limited to a wide range of side effects. The aim of the work is to study the effect of rectal suppositories of the original composition with vitamin D3 on the serum concentration of IgG, IgM and IL-8 in experimental UC. The work was performed on 70 white rats of the Wistar line, UC was modeled by two-stage administration of 3% oxazolone, vitamin D3 in the rectal suppositories of the original polyethylene glycol composition was used every 12 hours, in the comparison group rectal suppositories with 5-ASC were used. Serum concentrations of IL-8, IgG and IgM were determined using specific test systems for rats on an immunoassay analyzer on days 2, 4 and 6 of the experiment. It was established that in experimental oxazolone-induced ulcerative colitis in the serum of rats, the concentration of IL-8, IgG and IgM increases on days 2, 4 and 6 of the experiment. The use of vitamin D3 in the composition of rectal suppositories of the original composition daily with an interval of 12 hours with experimental UC leads to a decrease in the concentration of IL-8 for 6 days, and the concentration of IgG and IgM – on 4 and 6 days of observation.
Russian Journal of Immunology. 2019;22(2-2):885-887
pages 885-887 views

DYNAMICS OF DENSITY OF SPATIAL DISTRIBUTION OF MAST CELLS OF THE THYROID GLAND IN CONDITIONS OF INTERRUPTION OF LACTATION

Paraskun A.A., Vinogradov S.Y.

Abstract

It was established that under conditions of interruption of lactation in rats on the 2nd day of research, the total number of fat cells is maximum. Then (3‒7 days) the density of spatial distribution of tissue basophils decreases with a minimum of 7 days of observation. After 14 days, the number of mast cells increases, followed by a decrease of test days 21. These changes occur synchronously with the dynamics of the parameters of the morphofunctional activity of the gland.
Russian Journal of Immunology. 2019;22(2-2):888-890
pages 888-890 views

METABOLIC REPROGRAMMING OF MACROPHAGES ACTIVATED BY A NOD1 RECEPTOR AGONIST

Pashenkov M.V., Murugina N.E., Budikhina A.S., Maximchik P.V., Dagil Y.A., Balyasova L.S., Murugin V.V., Chkadua G.Z., Pinegin B.V.

Abstract

We provide the fi rst characterization of glucose and energy metabolism rearrangements in human macrophages upon their activation with a NOD1 receptor agonist (N-acetyl-D-muramyl-L-alanylD-isoglutamyl-meso-diaminopimelic acid, or M-triDAP) in comparison with the eff ects of a TLR4 agonist, lipopolysaccharide (LPS). We demonstrate possibilities of modulation of cytokine production by macrophages using glycolysis inhibitors.
Russian Journal of Immunology. 2019;22(2-2):891-893
pages 891-893 views

MORPHOLOGICAL AND FUNCTIONAL DIVERSITY OF MACROPHAGES OF DIFFERENT LOCALIZATION IN ANIMALS IN NORMAL AND IN CONDITIONS OF DIABETES MELLITUS TYPE I

Pozdina V.A., Danilova I.G.

Abstract

The study investigated the morphological and functional characteristics of macrophages of different localization, isolated from intact animals and animals with a model of diabetes mellitus type I. The study was performed on rat macrophage cultures isolated from the following areas: lungs, spleen, liver, and peritoneal cavity. The following morphometric parameters were determined: S cells, cytoplasm and nuclei, nuclear-cytoplasmic ratio (NCO). The phenotype was determined by the expression of CD163 and CD80. The macrophage cytokine activity was assessed by the level of IL-1α, IL-10 and TNFα. The morphological characteristics of macrophages from various organs were demonstrated and their functional differences were revealed.
Russian Journal of Immunology. 2019;22(2-2):894-896
pages 894-896 views

CONTENT OF ENDOTHELIAL PROGENITOR CELLS IN UMBILICAL CORD BLOOD IN PREECLAMPSIA

Popova I.G., Nazarov S.B., Kuzmenko G.N., Kroshkina N.V., Sitnikova O.G., Klycheva M.M.

Abstract

The content of endothelial progenitor cells in umbilical cord blood was determined in newborns born to mothers with preeclampsia and without preeclampsia. Phenotyping of umbilical cord blood endothelial cells was performed by flow cytometry, circulating endothelial cells (CES) were defined as: CD45, CD133+, VEGFR2+, CD34, endothelial progenitor cells (EPCS) were defined as: CD45, CD133+, VEGFR2+, CD34+. There was a decrease in both the total number of circulating endothelial cells and endothelial progenitor cells in premature infants compared to full-term ones. In newborns born to mothers with preeclampsia, only the number of ECP was significantly higher, compared with newborns from mothers without preeclampsia, and in the group of premature and full-term newborns. 

Russian Journal of Immunology. 2019;22(2-2):897-899
pages 897-899 views

LEVELS OF PERIPHERAL BLOOD CYTOKINES AND THE RISK FOR METABOLIC SYNDROME IN PATIENTS WITH STAGE II ESSENTIAL HYPERTENSION

Radaeva O.A., Simbirtsev A.S.

Abstract

The aim of this study was to investigate a correlation between the levels of IL-1β, IL-1α, IL-1ra, IL-18, IL-18BP, IL-37, IL-6, sIL-6r, LIF, sLIFr, IGF-1, IGFBP-1, TNF-α/sTNF-RI, sVCAM-1, IL-17, IL-2, IL-4, IL-10, TGF-β1, IL-8, CX3CL1, CXCL10, INFγ, M-CSF, IL-34, VEGF-A, ЕРО in the peripheral blood of patients with stage II EH and the rate of metabolic syndrome (MS) occurring in a 5-year follow-up period. Twenty-eight cytokines were measured using ELISA in the peripheral blood samples of 200 patients with stage II EH. The patients were followed up for 5 years to keep track of MS. The multivariate Cox regression analysis was applied to the following parameters: IL-1β>20,3 pg/ ml; LIF > 9,13 пг/мл; M-CSF>4538 pg/ml; IGF-1<116000 pg/ml. The analysis revealed that M-CSF> 453 pg/ml (р < 0.001) and IGF-1<116000 pg/ml (р < 0.001) correlated with an increase in the risk for MS in stage II EH.
Russian Journal of Immunology. 2019;22(2-2):900-902
pages 900-902 views

EFFECT OF MACROPHAGE CONDITION MEDIA ON PHENOTYPE AND PROLIFERATION OF HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y

Rashchupkin I.M., Tikhonova M.A., Tyrinova T.V., Sakhno L.V., Shevela E.Y.

Abstract

The study investigated the effect of conditioned media of M1 and M2 macrophages on the phenotype and proliferation of the human neuroblastoma cell line SH-SY5Y. It has been established that conditioned media of M2 macrophages, generated in the presence of GM-CSF under defi ciency of growth factors, have a pronounced stimulating effect on the SH-SY5Y cell proliferation. The expression of the low-affinity nerve growth factor receptor, CD271, correlates with cell morphology: in cultures with a predominance of N-type cells, the number of CD271+ cells is signifi cantly higher compared to cultures dominated by S-type cells.
Russian Journal of Immunology. 2019;22(2-2):903-905
pages 903-905 views

THE LEVEL OF PROINFLAMMATORY CYTOKINES IN PATIENTS WITH POSTOPERATIVE HYPOTHYRHYOSIS COMPLICATED BY HYDROPERICARD

Rybchinskaya I.I., Ivanov K.M., Ermolina E.V., Shkatova N.G.

Abstract

In 46 women out of 136 (mean age 48.0 ± 2.3 years) who were operated on for a nodular goiter, a pericardial effusion and elevated serum levels of IL-6 and TNF-α were detected before the operation. After the operation, as the volume of hydropericardium increased, an increase in the serum cytokine content was observed with a direct correlation (r = 0.39; р<0.05) between TNF-α and the volume of effusion in the pericardial cavity.
Russian Journal of Immunology. 2019;22(2-2):906-908
pages 906-908 views

PROTEIN EXTRACTS FROM BEANS OF SOY AND PEANUT: FRACTIONAL COMPOSITION AND ALLERGENIC PROPERTIES

Samoylikov P.V., Berzhets V.M.

Abstract

Soybeans and peanuts are the strongest food allergens among all leguminous plants. Today, allergens of these legumes are not sufficiently accessible in clinical practice for a number of reasons. In this regard, the aim of the work was to obtain protein extracts from soybeans and peanuts and to evaluate their biochemicaland allergenic properties. The preparation of a solution of protein-polysaccharide complexes was performed by the method of water-salt extraction, the determination of the protein content was carried out by the method of Bradford, the study of the protein composition of the obtained extracts was carried out by the polyacrylamide gel electrophoresis. Allergenic properties were investigated by the method of inhibition of sIgE binding. Proteins were described in the obtained protein extracts. They belong to cupin, prolamin, profi lin protein fractions and Bet v 1-like proteins. A study of individual protein fractions and further comparison of their allergenic properties will significantly complement the component diagnostics, which is actively being introduced into clinical practice.
Russian Journal of Immunology. 2019;22(2-2):909-911
pages 909-911 views

EVALUATION OF THE EFFECT OF ENDONUCLEASE ON EXTRACELLULAR NEUTROPHILIC TRAPS

Samuseva I.V., Savochkina A.Y., Nikushkina K.V., Yemelyanov I.V., Nikonova T.I., Zotova M.A.

Abstract

The paper presents the results of the analysis of the dynamics of the formation of extracellular DNA networks by neutrophils isolated from peripheral blood and their fragmentation. During the experiment, it was found that deoxyribonuclease I (DNase I) cleaves DNA – the basis of extracellular traps. The ability to secrete this enzyme component in some pathogenic bacteria (S. pyogenes, S. aureus or S. pneumoniae) probably explains their ability to release from extracellular structures.
Russian Journal of Immunology. 2019;22(2-2):912-914
pages 912-914 views

POPULATION COMPOSITION OF NEUTROPHILS IN THE TUBERCULOUS PROCESS

Serdyuk Y.V., Nenasheva T.A., Nikitina I.Y., Karpina N.L., Lyadova I.V.

Abstract

Neutrophils are the first line of defense against infectious diseases. The contribution of neutrophils to the pathogenesis of tuberculosis is controversial and not fully understood. In this study, we analyzed the heterogeneity of neutrophil populations in patients with tuberculosis and control subjects. In patients with tuberculosis, there is an increase in the number of low-density neutrophils compared with control subjects. We have found that the low-density neutrophil population is heterogeneous and contains a subpopulation of activated cells.
Russian Journal of Immunology. 2019;22(2-2):915-917
pages 915-917 views

THE ROLE OF B1 AND B2 LYMPHOCYTES IN THE FORMATION OF DIFFERENT VARIANTS OF RHEUMATOID ARTHRITIS

Sizyakina L.P., Kharitonova M.V.

Abstract

The results of the phenotypic characteristics of B1 and B2 lymphocytes of patients with seropositive (51 people) and seronegative (41 people) variants of the course of articular form of rheumatoid arthritis are presented. An increase in the content of B1 lymphocytes in both seronegative and seropositive variants was shown. A distinctive feature of B2 lymphocytes in the seropositive compared with the seronegative option is to enhance the activation processes and decrease the intensity of apoptosis with the progression of the autoimmune process.
Russian Journal of Immunology. 2019;22(2-2):918-920
pages 918-920 views

INFLUENCE OF DIFFERENT INTERFERON-GAMMA (IFN-γ) CONCENTRATIONS ON CELLS OF THE IMMUNE SYSTEM IN VITRO

Sitkovskaya A.O., Sagakyants A.B., Filippova S.Y., Mezhevova I.V., Bondarenko E.S., Statsenko K.G., Popova M.M.

Abstract

The work was aimed at determining the possibility of activation of lymphocytes and natural killer cells with the addition of various concentrations of IFN-γ in vitro. The results showed that IFN- γ promotes maturation of T eff ector cell-mediated immunity system.
Russian Journal of Immunology. 2019;22(2-2):921-923
pages 921-923 views

ONCOLYTIC REOVIRUS R-92: EFFECT IN THE GENERATION OF MATURE DENDRITIC CELLS (RESEARCH IN VITRO)

Sitkovskaya A.O., Mezhevova I.V., Bondarenko E.S., Kolpakov S.A., Kolpakova E.P., Karmirgodiev A.A., Panchenko P.S.

Abstract

Study was conducted of the effect of reovirus strain R-92 on the maturation of dendritic cells (DC). Work was carried out on PBMCs from the peripheral blood of healthy donors. The results showed that the presence of reovirus strain P-92 at the stage of differentiation of immature DC into a mature state leads to increased expression of key markers of mature DC.
Russian Journal of Immunology. 2019;22(2-2):924-926
pages 924-926 views

CONDITION OF JAK-STAT SYSTEM IN PURULENT-INFLAMMATORY DISEASES OF SOFT TISSUES

Snimschikova I.A., Khabina N.A., Plotnikova M.O., Afonina I.A., Chestnihina A.D.

Abstract

Characterization of impaired JAK-STAT-signal transduction and SOCS regulation in cytokine-dependent activation in purulent-inflammatory diseases of soft tissues.
Russian Journal of Immunology. 2019;22(2-2):927-929
pages 927-929 views

MANIFESTATION OF REGENERATORY POTENCIAL OF EXTRA-ISLET INSULIN-PRODUCING PANCREATIC CELLS AT THE EXPERIMENTAL DIABETES TYPE2 AND AT ADMINISTRATION OF 3-AMINOPHTALHYDRASIDE

Sokolova K.V., Gette I.F., Emelyanov V.V., Abidov M.T.

Abstract

Restoration of quantity, size of cells and their functional activity reflect the possibility of tissue to regenerate. 3-aminophtalhydrazide (APH), which effects to the macrophage activation: classic vs. alternative, (М1→М2), increases quantity and sizes of insulin-producing cells, localized in pancreatic ducts. Besides, APH leads to increase of optical density of insulin both in beta-cells and insulin-producing cells, located in acinar part of the pancreas and in ducts.
Russian Journal of Immunology. 2019;22(2-2):930-932
pages 930-932 views

THE STUDY OF ABILITY OF DENDRITIC CELLS LOADED WITH AUTOLOGICAL TUMOR LIZATE OF PATIENT SWITHHIGH-GRADE GLIOMA TO ACTIVATE ANTIGEN-SPECIFIC T-CELL RESPONSE IN VITRO

Soloveva E.D., Tyrinova T.V., Kalinovsky A.V., Mishinov S.V., Chernov S.V., Stupak V.V.

Abstract

In the present work we demonstrated that IFNα-induced dendritic cells (IFN-DCs) loaded with a lysate of autologous tumor cells of high-grade glioma patients (DCLYS) more eff ectively stimulated the proliferative response of autologous T-cells compared with unloaded IFN-DCs (DCLPS) and did not induce apoptosis of CD4+ and CD8+ T cells. Moreover, DCLYS activated cytotoxic CD8+ T cells (in the degranulation test) in cultures of autologous mononuclear cells of studied patients. 

Russian Journal of Immunology. 2019;22(2-2):933-935
pages 933-935 views

LYMPHOCYTE SUBPOPULATION PROFILE IN PATIENTS WITH METABOLIC SYNDROME AND IT’S CHANGE UNDER THE INFLUENCE OF ADIPOKINS IN EXPERIMENTAL CONDITIONS

Sumerkina V.A., Telesheva L.F., Golovneva E.S., Baturina I.L., Naimushina Y.V.

Abstract

An important role in the regulation of immune interactions in metabolic syndrome (MS) belongs to leptin and adiponectin, their levels in peripheral blood are well known, while the specific effects of these mediators on human lymphocytes are not well understood. In this work, we evaluated the subpopulations of lymphocytes in patients with metabolic syndrome in vivo, and studied the expression of activation markers of peripheral blood lymphocytes after incubation with leptin and adiponectin at concentrations close to those of metabolic syndrome.
Russian Journal of Immunology. 2019;22(2-2):936-938
pages 936-938 views

MODEL OF INFLUENCE HYPOSCIAS ON THE CELLULAR COMPONENT AND SYNTHESIS COMPONENTS EXTRACELLULAR MATRIX IN CULTURE FIBROBLASTS

Suhovej U.G., Kostolomova E.G., Unger I.G., Akuneeva T.V., Aptekar’ I.A.

Abstract

It is established, that cultivation fibroblasts in conditions hypoxia leads to decrease in their morphological heterogeneity, increase proliferative to activity and viability of cells. Causes oppression of synthesis of components extracellular matrix (collagen and elastin) and decrease expression a receptor hyaluronic acids (CD44). Duration hypoxia causes a degree of decrease.
Russian Journal of Immunology. 2019;22(2-2):939-941
pages 939-941 views

EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTORS 1 (VEGFR-1) AND THEIR ROLE IN THE REGULATION OF T CELL PROLIFERATION

Tashkina E.A., Leplina O.Y., Batorov E.V., Ostanin A.A., Pasman N.M.

Abstract

The expression of vascular endothelial growth factor receptors-1 (VEGFR-1) on CD4+ and CD8+ donor T lymphocytes in mononuclear cell (MNC) cultures, as well as the eff ect of placental growth factor (PlGF) on T cell proliferation have been investigated. Freshly isolated T cells had low expression of VEGFR-1, which increased during spontaneous cultivation of T cells and most strongly upon activation of T lymphocytes with aCD3 antibodies. The maximum increase in VEGFR-1 – positive CD4+ and CD8+ T cells is observed at 48 hours. PlGF in a wide dose range inhibits the proliferation of aCD3- stimulated T cells, suppressing CD4+ and CD8+ T lymphocyte division to the same extent. 

Russian Journal of Immunology. 2019;22(2-2):942-944
pages 942-944 views

DENDRITIC CELLS ELECTROPORATION WITH DNA-PLASMID ENCODING IL-10

Tereshchenko V.P., Khantakova J.N., Kurilin V.V., Silkov A.N., Maksyutov R.A.

Abstract

Dendritic cells (DC) are widely used to produce vaccines for the treatment of various types of diseases, such as malignant neoplasms, autoimmune disorders, rejection and GVHD. To control the functional properties of DCs, they are transfected with various immunoregulatory proteins, including IL-10. In this paper, the optimization of the method of electrical transfection (electroporation) of DCs with a DNA plasmid encoding IL-10 was performed. The effi ciency of DC electroporation (EP) reaches 45‒72% with cell death below 7%. The active expression of IL-10 in transfected DC was shown within 3 days after EP. According to the results of the work, EP was recognized as an eff ective method for obtaining transfected DCs for the production of cellular vaccines.
Russian Journal of Immunology. 2019;22(2-2):945-947
pages 945-947 views

INTERNET DEPENDENCE IN ADOLESCENTS OF CENTRAL SIBERIA

Tereshchenko S.Y., Smolnikova M.V.

Abstract

Pathological Internet addiction belongs to the group of multifactorial polygenic diseases, where in each case there is a unique combined effect of the innate features of the functioning of higher nervous activity, some of which are genetically determined with the influence of many environmental factors. In this regard, one of the most important fundamental tasks is the identifi cation of specific genes and neurotransmitter associations responsible for the propensity to form Internet addiction in adolescents, which will lead to a search for new therapeutic targets and ways of early prevention with an assessment of the degree of genetic risk.
Russian Journal of Immunology. 2019;22(2-2):948-950
pages 948-950 views

THE DETERMINATION OF MMP-2, TIMP-2 LEVELS AND ITS RATIO IN BLOOD SERUM IN PATIENTS WITH AKI BEFORE END AFTER CORONARY ARTERY BYPASS GRAFTING

Fisenko V.G., Fomina O.G., Boyarskaya P.N., Paschenko A.L., Chagina E.A.

Abstract

The levels of MMP-2, TIMP-2 and its ratio in blood serum were studied in order to identify predictors of severe AKI for initiating early renal replacement therapy after CABG. The study engaged 60 patients, from 45 to 74 years old, with CHD before and after CABG without diabetes mellitus. Statistically signifi cant differences of MMP-2, TIMP-2 and its ratio in the blood serum were revealed. Determining the ratio of two structural kidney damage markers (MMP-2, TIMP-2) in patients on the 2nd day after CABG allows to detect AKI. The dynamics of the MMP-2 / TIMP-2 ratio in the two compared groups with AKI was different. In the 1st group, the value of ratio signifi cantly decreased on the first day after CABG, significantly increased on the 2nd and reached high rates on the 7th day. In the 2nd group, there was a wave dynamics of MMP-2 / TIMP-2 ratio: increase on the first day, a signifi cant decrease on the 2nd and a twofold increase on the 7th day. Diagnostic and prognostic significance of the MMP-2 level was found in patients with CKD at all steps of monitoring.
Russian Journal of Immunology. 2019;22(2-2):951-953
pages 951-953 views

IMMUNE-ENDOCRINE AND PSYCHOLOGICAL MECHANISMS OF ADAPTATION AGAIN A FORMED COLLECTIVE

Fisher T.A., Petrov S.A., Fisher I.V.

Abstract

The article provides the rationale for new methodological approaches to the study of adaptation mechanisms in terms of functional conjugacy of psycho-immune-endocrine parameters in the context of the stages of a specific immune response.
Russian Journal of Immunology. 2019;22(2-2):954-956
pages 954-956 views

EFFECT OF TRANSPLANTATION OF MESENCHIMAL STEM CELLS (MSC) AND PRODUCED MICROVESICLES (MV) ON THE MAINTENANCE OF MEMORY T-CELLS AND T-REGULATORY CELLS IN THE MODEL ACUTE RENAL FAILURE IN MICE

Khabalova T.S., Kaschenko E.A., Seledtsova G.V.

Abstract

MV has been shown to have a positive effect on the restoration of renal excretory function at a level comparable to the effect of MSCs. The use of MSC and MB in the treatment of acute renal failure resulted in almost the same result: on the 4th day the level of urea was 9.3 μg/ml and 9.37 μg/ml, respectively, which is 25% lower than in the glycerol control. By the 8th day, the level of urea was 7.9 μg/ ml after the introduction of MSC and 7.6 μg/ml after the introduction of MB, which is 21% lower than in the glycerol control. It has been shown that the MW produced by MSCs stimulate the proliferation of both splenocytes and cells of the kidney tissue in a dose-dependent manner. To study the resistance to apoptosis, splenocytes were used both from intact mice and from glycerol-induced ARF. The addition of MB causes a decrease in the level of apoptosis in both intact mice (21.73%) and mice with ARF (13.28%). Accordingly, inversely, the percentage of cells in the S / M phase of the cell cycle increased.
Russian Journal of Immunology. 2019;22(2-2):957-959
pages 957-959 views

EFFECT OF TRANSPLANTATION OF MESENCHIMAL STEM CELLS (MSC) AND PRODUCED MICROVESICLES (MV) ON THE MAINTENANCE OF MEMORY T-CELLS AND T-REGULATORY CELLS IN THE MODEL ACUTE RENAL FAILURE IN MICE

Khabalova T.S., Kaschenko E.A., Seledtsova G.V.

Abstract

The aim of the work was to study the effect of mesenchymal stem cells (MSCs) and microvesicles (MV) produced by them on the parameters of cellular immunity in mice with induced ARF. The experiments were carried out on C57Bl6 linear mice aged 3‒4 months. Acute renal failure (ARF) was induced by a single injection of 50% glycerol. Mesenchymal stem cells were obtained from the bone marrow of healthy animals, cultured under standard conditions. The study included such parameters of cellular immunity as memory T-cells and T-regulatory cells. L929 cells and microvesicles derived from them (L929-МV) were used as controls. A tendency towards a spontaneous decrease in the content of CD4+CD44+CD62L+, CD8+CD44+CD62L+ T-cells of memory and regulatory CD4+CD25+FoxP3+ cells was recorded. 11 days of the experiment. The effects of the application of cells and microvesicles of them differed in the severity of the impact on the cells of the immune system. L929-MV had a more pronounced effect on the 11th day of the experiment. In the course of the experiment, a tendency was observed for the growth of regulatory CD4+CD25+FoxP3+ T cells using both MSC and L929, and microvesicles derived from them. The nature of the cells used and the microvesicles obtained from them did not have a significant effect on the final result of the experiments. 

Russian Journal of Immunology. 2019;22(2-2):960-962
pages 960-962 views

CYTOKINE PROFILE OF SERUM AND TONSILS IN CHILDREN WITH CHRONIC TONSILLITIS IN THE BACKGROUND OF HERPESVIRUS INFECTIONS

Kharaeva Z.F., Azamatova E.K., Balaeva J.T., Tlakadugova M.T., Shomakhova Z.A.

Abstract

The study included children with chronic tonsillitis in the background cytomegalovirus or virus Epshtein-Barr infection. IL-1β, IL-6, TNF, IL-10 in blood serum and washes from tonsils were studied as laboratory parameters of local and general cytokine status. Imbalance of pro-inflammatory and antiinfl ammatory cytokines in the group of patients with chronic tonsillitis was revealed. Concomitant herpes virus infection exacerbates tendencies, leading to more meaningful local violation of the anti-microbial protection.
Russian Journal of Immunology. 2019;22(2-2):963-965
pages 963-965 views

CONTENT OF GALECTINS-2, -9 AND SUBPOPULATIONS OF BLOOD MONOCYTES IN PATHOLOGY OF LUNG INFECTIOUS AND NON-INFECTIOUS GENESIS

Chumakovа S.P., Vins M.V., Urazovа O.I., Bukreeva E.B., Bulanova A.A., Churinа E.G., Sitnikovа A.V., Novitsky V.V.

Abstract

15 patients with infiltrative pulmonary tuberculosis (TB), 16 patients with chronic obstructive pulmonary disease (COPD) and 12 healthy donors were examined. Amount of CD14hiCD16, CD14hiCD16lo, CD14loCD16lo and CD14loCD16 monocytes in the blood was evaluated by flow-cytometry, and galectin-2 and -9 concentration was measured by ELISA. Increase in the number of CD14hiCD16lo monocytes, differentiated from the CD14hiCD16 cells, and decrease in the content of galectin-2 and CD14lo CD16 monocytes in the blood were characteristic of TB. In COPD, an increase in the concentration of galectin-9 and the proportion of CD14hiCD16 monocytes against the background of deficiency of other their subpopulations in the blood was noted. 

Russian Journal of Immunology. 2019;22(2-2):966-968
pages 966-968 views

THE ROLE OF THE ENDOGENOUS OPIOID SYSTEM IN THE REGULATION OF ANTIBODY GENESIS DURING CHRONIC COLD STRESS

Sharav’eva I.L., Gein S.V.

Abstract

It was established that under conditions of chronic cold stress in mice, a decrease in the absolute and relative amount of antibody-forming cells in the spleen was observed. The blockade of opiate receptors led to the abolition of oppression.
Russian Journal of Immunology. 2019;22(2-2):969-971
pages 969-971 views

EFFECTS OF ACUTE COLD STESS ON IFNγ AND IL-12 PRODUCTION BY MICE SPLENOCYTES

Sharav’eva I.L., Tendryakova S.P.

Abstract

The effect of cold stress on the production of reactive oxygen species (ROS) and IFN-γ and IL-12 was studied 6 hours after the end of stress. It was established that acute cold stress (–20 °C for 60 min) led to increased spontaneous and zymosan-stimulated production of ROS by mouse peritoneal cavity cells 6 hours after the end of stress exposure. In similar experimental conditions, the levels of interferon-γ in spontaneous splenocytes mice increased, while in stimulated mice decreased.
Russian Journal of Immunology. 2019;22(2-2):972-974
pages 972-974 views

IMMUNE STATUS IN ACUTE OBSTRUCTIVE AND NONOBSTRUCTIVE PYELONEPHRITIS

Shatokhin M.N., Mavrin M.Y., Ragulina V.A., Khorlyakov K.V.

Abstract

The patients with a diagnosis of acute non-obstructive (ANOP) and obstructive pyelonephritis (AOP) (31 and 30 patients respectively) were under observation during treatment at the urological center of the National Healthcare Institution Scientifi c Clinical Center of Russian Railways. The levels of cytokines, complement components and their inhibitors in plasma and the first portion of morning urine, phagocytic and oxygen-dependent activity of circulating blood neutrophils were determined. At the systemic level, in patients with ANOP, to a greater extent in AOP, an increase in the concentration of pro-inflammatory cytokines (TNFα, IL-6, IL-8 and IFNγ), IFNα, IL-4 and IL-10, components of the C3 and C5a compliment was established, a decrease in the level of IL-1RA and a regulator of the complement factor H system, a decrease in the activity and intensity of phagocytosis of peripheral blood neutrophils with an increase in the activity of oxygen-dependent systems were also revealed. Before treatment at the local level (in the urine) in patients with ANOP, an increase in the concentration of all studied cytokines, except IL-4, components C3 and C5, and a decrease in IL-1RA was established. In AOP, changes were more pronounced, with the exception of a reduced level of factor H and sIgA. 

Russian Journal of Immunology. 2019;22(2-2):975-977
pages 975-977 views

PHARMACOLOGICAL IMMUNOREHABILITATION PATIENTS WITH OBSTRUCTIVE LUNG DISEASE IN THE REGION OF KURSK MAGNETIC ANOMALY

Shelukhina A.N., Konoplya E.N., Gavrilyuk E.V., Gavrilyuk V.P.

Abstract

The study included 76 patients with chronic obstructive pulmonary disease aged 35 to 50 years (mean age 42,5±2,7 years), of which 38 patients resided permanently in Zheleznogorsk, and the other 38 patients in Kursk. The use of licopid in patients with chronic obstructive pulmonary disease in Kursk allowed to correct the concentration of IL-10, INFγ, whereas in residents of Zheleznogorsk it normalizes the IgM level and corrects the concentration of TNFα, IL-8, IL-10, but not to the normal level.
Russian Journal of Immunology. 2019;22(2-2):978-980
pages 978-980 views

INFLUENCE OF OPSONINS OF FRESH AND INACTIVATED UNDER 56 °C BLOOD SERUM ON ZYMOSAN-INDUCED PRODUCTION OF REACTIVE OXYGEN SPECIES BY RAT LEUCOCYTES

Shilov J.I., Godovalov A.P., Shilov D.J., Shilov S.J., Eliáš D.

Abstract

The production of reactive oxygen species by blood leukocytes and bone marrow cells in the reaction of luminol-dependent chemiluminescence under the action of non-opsonized zymosan and zymosan opsonized with fresh or inactivated autologous serum at 56 °C was investigated. It was established that an increase in the chemiluminescence levels takes place in all three variants of phagocyte activation, but it is most pronounced when using fresh serum.
Russian Journal of Immunology. 2019;22(2-2):981-983
pages 981-983 views

CHANGES IN PHAGOCYTOSIS PARAMETERS WITH A MARKER OF PHAGOSOME ACIDIFICATION pHrodo™ UNDER-ZYMOSAN-INDUCED PERITONITIS IN RATS

Shilov S.J., Shilov J.I., Tulyaev Y.A., Barkov S.Y.

Abstract

The changes in phagocytosis under 12-hour zymosan-induced peritonitis in rats were investigated using flow laser cytometry using zymosan labeled with pH-sensitive flurochrome green pHrodo™ (Thermo Fisher Scientifi c). An increase in the absolute parameters of phagocytosis was found in the study of peritoneal exudate cells, blood leukocytes and bone marrow cells in rats with peritonitis in comparison with intact animals.
Russian Journal of Immunology. 2019;22(2-2):984-986
pages 984-986 views

GLYCOARRAYS FOR STUDYING OF GLYCAN-PROTEIN INTERACTION

Shilova N.V., Khasbiullina N.R., Nokel A.Y., Ziganshina M.M., Bovin N.V.

Abstract

The capacities of glycoarray – a unique method for investigation of carbohydrate-binding proteins including antibodies and lectins – are presented. It was shown that array is very flexi ble device and it can be directional changed for solution of a wide range of diff erent tasks of both fundamental and applied nature.
Russian Journal of Immunology. 2019;22(2-2):987-989
pages 987-989 views

REGULATORY CD4+ T CELLS ISOLATED FROM NAIVE, MEMORY AND EFFECTOR LYMPHOCYTE SUBSETS KEEP THE PROPERTIES OF APPROPRIATE SUBPOPULATIONS

Shmagel K.V.

Abstract

In the blood of healthy volunteers, CD4+ T-lymphocyte subsets (naïve cells, central and eff ector memory cells, terminally diff erentiated effectors) were determined by flow cytometry. Using CD25, CD127, and FoxP3 molecules, regulatory T-lymphocytes (Tregs) were identified within each subset. Mitochondrial mass and charge, as well as the expression of CD71, CD39, GARP, LAP, and transcriptional co-activator PGC-1α regulating energy metabolism were assessed within each subset’s total cell pool and Tregs isolated from the appropriate subset. Correlation analysis showed that the Treg activation phenotype reflects the one of the parent subset. Furthermore, strong links between the energy indices of Tregs and the appropriate T-cell subset were established. 

Russian Journal of Immunology. 2019;22(2-2):990-993
pages 990-993 views

RELATIONSHIP OF IMMUNE AND METABOLIC DISORDERS IN CHRONIC ISCHEMIA OF THE BRAIN

Shulginova A.A., Ryzhikova G.N., Colomoets I.I., Ragulina V.A.

Abstract

The study included 104 patients hospitalized in the neurological department of the Kursk Regional Clinical Hospital with chronic cerebral ischemia (CCI) against the background of hypertension of the II stage, equally divided by the I and II stages of the disease. In the blood (plasma, neutrophils and erythrocytes), the parameters of the immune and metabolic status were determined with an assessment of the relationship between the studied parameters. In patients with CCI stage I and stage II it is established that there are more reliable interrelations in patients with early stage of the disease compared with those in patients with stage II, and this probably indicates a greater disadaptation of the mechanisms for maintaining immune and metabolic homeostasis in patients with CCI stage II, compared with patients with stage I disease.
Russian Journal of Immunology. 2019;22(2-2):994-996
pages 994-996 views

COMPARATIVE CHARACTERIZATION OF MMR-9 AND TIMP-1 PRODUCTION BY DIFFERENT MACROPHAGES SUBSETS

Yankovskaya A.A., Sakhno L.V., Shevela E.Y.

Abstract

The paper presents a comparative characterization of the MMP-9 and TIMP-1 production by various subsets of macrophages (Mφ) differentiated in the presence of GM-CSF and polarized into M1, M2a and M2c cells. It was shown that all the studied Mφ subtypes produced both MMP-9 and TIMP-1. At the same time, the MMP-9 / TIMP-1 ratio was signifi cantly higher in M2c Mφ compared to M1 and M2a, which may indicate the important role of M2c macrophages during tissue remodeling and restriction of fibrosis.
Russian Journal of Immunology. 2019;22(2-2):997-998
pages 997-998 views

IMMUNOGENICITY OF DNA VACCINE CONSTRUCTS ENCODING EBOLA VIRUS CYTOTOXIC AND T-HELPER EPITOPES

Bazhan S.I., Antonets D.V., Dudko S.G., Kaplina O.N., Karpenko L.I., Ilyichev A.A.

Abstract

The lack of effective vaccines against Ebola virus initiates a search for new approaches to solving this problem. One of the promising directions in development of antiviral vaccines based on designing DNA-vaccines encoding artificial polyepitope immunogens. Previously, using the original software, we designed two polyepitopic T-cell immunogens containing cytotoxic and T-helper epitopes of Ebola virus and showed that DNA vaccines based on them provide synthesis of target mRNA and proteins in a culture of eukaryotic cells. As part of this work, we have shown that engineered DNA vaccine constructs induce virus-specific responses of CD4+ and CD8+ T-lymphocytes in immunized mice. 

Russian Journal of Immunology. 2019;22(2-2):1000-1002
pages 1000-1002 views

DEVELOPMENT OF A CANDIDATE SYNTHETIC VACCINE FOR IMMUNOTHERAPY OF MELANOMA

Borobova E.A., Antonets D.V., Starostina E.V.

Abstract

Melanoma is the most dangerous skin cancer. It’s characterized by rapid growth rates and high incidences of metastasis. Melanoma cells have tumor antigens on their surfaces. Thereby, it’s possible to create an eff ective vaccine against melanoma. Earlier in the Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector» DNA-constructs, encoding artifi cial polyepitope immunogens MEL-A0201 and MEL-TCI, that consist of cytotoxic and helper T-cell epitope form six melanoma antigens, were created. This article includes the results of evaluation of the capability of designed DNA-constructs to induce antitumor immune response.
Russian Journal of Immunology. 2019;22(2-2):1003-1005
pages 1003-1005 views

SEASONAL INFLUENZA VACCINE STRAIN RECOMMENDATIONS IN RECENT YEARS

Vasiliev Y.M.

Abstract

World Health Organization (WHO) experts issue strain recommendations to ensure effective yearly influenza vaccination. Several important “firsts” had taken place in recent years. An up to a 1-month recommendation delay puts the whole system of timely procurement of enough doses of effective and safe influenza vaccines based on up-to-date strains at risk.
Russian Journal of Immunology. 2019;22(2-2):1006-1008
pages 1006-1008 views

PERSPECTIVES OF IMMUNE ADJUVANT PLATFORMS WITH ADJUSTABLE PROPERTIES FOR INFLUENZA VACCINES

Vasiliev Y.M.

Abstract

Use of adjuvants is among the most promising approaches for enhancement of vaccines for the control of infectious diseases. Animal experiments with various influenza vaccines have shown that platforms of adjuvants with adjustable properties are very promising (suspensions, oil-in-water emulsions, chitosanbased formulations) because vaccine type and administration route are taken into account.
Russian Journal of Immunology. 2019;22(2-2):1009-1011
pages 1009-1011 views

RECOMBINANT PNEUMOLYSIN INDUCES THE FORMATION OF ANTIBODIES AND PROTECTS MICE FROM PNEUMOCOCCAL INFECTION

Vorobyev D.S., Petukhova E.S., Sidorov A.V., Semenova I.B., Volokh Y.V., Leonova A.Y., Mikhailova N.A.

Abstract

The obtained recombinant pneumolysin (rPLY) Streptococcus pneumoniae in atoxic form. The immunogenic and protective activity of rPLY was studied. It was shown that in the sera of mice after double injection of rPLY, the IgG titer increased by up to 32 times as compared with the sera of intact mice. After the third administration of rPLY, the IgG titer increased by up to 15 times compared with the initial IgG level after the second immunization. The protective activity of rPLY revealed in the experience of active protection in intraperitoneal infection of S. pneumoniae mice.
Russian Journal of Immunology. 2019;22(2-2):1012-1014
pages 1012-1014 views

OBTAINING AND PRECLINICAL STUDIES OF RECOMBINANT PSEUDOMONAS VACCINE

Mikhailova N.A., Kaloshin A.A., Zimina E.M., Kalinichenko E.O., Soldatenkova A.V., Plekhanova N.G., Victorov L.V.

Abstract

Three experimental lots of Recombinant Pseudomonas Vaccine (RPV) consisting a complex of the recombinant outer membrane protein F (OprF) and the recombinant toxoid that were sorbed on aluminum hydroxide have been obtained. During preclinical studies, we confi rmed the immunogenicity of the vaccine that had not pyrogenicity, acute and chronic toxicity, allergenicity and immunotoxicity.
Russian Journal of Immunology. 2019;22(2-2):1015-1017
pages 1015-1017 views

ACTUAL PROBLEMS OF VACCINE PROPHYLAXIS OF HPV-RELATED DESEASES

Nikitina T.N., Zhuk I.E., Klimov V.I., Kozlova T.Y., Gorenkov D.V.

Abstract

Human papillomavirus (HPV) is the most common viral infection of the reproductive tract. It can cause HPV-related diseases that lead to serious health problems. Persistent high-risk (HR) human papillomavirus infection is necessary for development of cervical cancer. Vaccination is an eff ective method to prevent HPV-related diseases. The world experience in using of HPV vaccines has shown their safety and high eff ectiveness.
Russian Journal of Immunology. 2019;22(2-2):1018-1020
pages 1018-1020 views

PECULIARITIES OF IMMUNE RESPONSE TO MEASLES VACCINATION IN SERONEGATIVE ADULTS

Smerdova M.A., Andreev Y.Y., Toptygina A.P.

Abstract

100 healthy adults aged 18–30 years were examined for the presence of measles immunity. A protective level of measles antibodies was found in 32%, and in 68% of adults such antibodies were not detected. Specific cellular immunity to measles virus antigens was detected in 57.37% of the examined individuals, in 26.07% of them specific cellular immunity to measles viruses was not detected, and in 6.56% the response was regarded as equivocal. Seronegative adults were vaccinated with measles cultural live vaccine (“Vector”, Russia). After 6 weeks, protective levels of anti-measles antibodies were detected in all vaccinated patients, the average level was 2.18 (1.40‒3.68) IU/ml. Specific cellular immunity to antigens of measles viruses in 3 people was regarded as equivocal, in the rest of the vaccinated as positive.
Russian Journal of Immunology. 2019;22(2-2):1021-1023
pages 1021-1023 views

IMMUNOGENICITY OF DNA VACCINE CONSTRUCTS ENCODING INFLUENZA VIRUS ARTIFICIAL ANTIGENS

Starostina E.V., Kaplina O.N., Karpenko L.I., Dudko S.G., Bazhan S.I.

Abstract

Development of a universal influenza vaccine is an important task. One of approaches for develop a universal influenza vaccine is design of artificial immunogens, which consist of fragments from proteins of diff erent subtypes of influenza virus. Candidate universal DNA-vaccine were design in the FBSI SRC VB «Vector» earlier. The vaccine encode artificial antigens designed on the basis of conservative fragments of the hemagglutinin stem from influenza A virus of two subtypes H1N1 and H3N2, and on the basis of conservative protein M2. The article presents results of a study of the T-cell immune response in mice that were immunized with DNA-vaccine constructs encoding the designed influenza virus antigens.
Russian Journal of Immunology. 2019;22(2-2):1024-1026
pages 1024-1026 views

QUESTIONS OF SOCIALIZATION OF PATIENTS WITH PID

Zaitsevа E.V., Bolkov M.A., Voronina L.I.

Abstract

Expanding scientific and clinical knowledge of primary immunodeficiencies allows diagnosing patients with primary immunodeficiencies at earlier ages. Thanks to this, patients get the opportunity to live a full and long life. This circumstance actualizes the need to solve problems arising in the socialization of children and adolescents with PID. Is necessary to create conditions for such children to receive education, to master the skills of social communication, while minimizing the risks of psychological trauma associated with the side effects of the disease. Solving the problems of socialization of children and children with PIDs who have limited health capabilities becomes relevant.
Russian Journal of Immunology. 2019;22(2-2):1028-1031
pages 1028-1031 views

SCREENING FOR HEREDITARY DISEASES: A BRIEF LOOK AT DOMESTIC AND FOREIGN EXPERIENCE IN LEGAL REGULATION

Nechkin A.V.

Abstract

Domestic legislation is familiar with the concept of genodiagnostics, however, the most important issues related to its conduct (about permitted and prohibited to conduct varieties of genodiagnosis, guarantees of non-discrimination and confi dentiality following this procedure, about the fate of genetic samples remaining after it) remain unresolved. Within the framework of this article, the author examines examples of the normative regulation of the issues mentioned in foreign countries, suggesting that foreign experience should be used wisely.
Russian Journal of Immunology. 2019;22(2-2):1031-1033
pages 1031-1033 views

DEVELOPMENT OF TECHNOLOGIES OF GENETIC RESEARCH AND CONSULTATION ON THE IDENTIFICATION OF CONGENITAL IMMUNITY ERRORS: PROBLEMS AND THEIR SOLUTIONS

Tuzankina I.A., Voronina L.I., Zaitsevа E.V.

Abstract

The authors provide a theoretical justification for the concepts of “technology of genetic research” and “technology of counseling based on the diagnosis of severe combined immunodefi ciency.” This type of technology is considered as a combination of such elements as ethical norms, an algorithm for diagnostic actions and consulting.
Russian Journal of Immunology. 2019;22(2-2):1034-1037
pages 1034-1037 views

REGIONAL REGISTER OF PRIMARY IMMUNODEFICIENCES OF THE REPUBLIC OF BASHKORTOSTAN

Khairullina R.M., Prolygina D.D., Kalmetyeva L.R., Davletbaeva G.A., Mirsayapova I.A., Makarova G.U., Ibragimova L.G.

Abstract

Primary immunodeficiency (PID) is a diverse group of genetically determined diseases, including more than 330 diseases. Early detection of PID and selection of adequate therapy aff ects the prognosis of the disease, improves the quality of life and life expectancy of patients significantly. PID register is the main tool for solving the tasks. Registration of patients with PID in the Republic of Bashkortostan (RB) is carried out on the basis of the Republican Children Clinical Hospital (RCCH) of Ufa and at March 2019, the number of registered patients increased to 223 people. Currently, 126 children are observed in the RCCH of Ufa, 43 patients have been transferred to the adult network. Distinctive features of the regional register of the RB are the presence of patients for whom the diagnosis of PID was set at autopsy and the absence of patients with Nij megen syndrome. The solution of current problems related to the diagnosis and treatment of PID is possible only with state support and teamwork of doctors of various specialties.
Russian Journal of Immunology. 2019;22(2-2):1038-1040
pages 1038-1040 views

EXPRESSION OF IMMUNE RESPONSE GENES IN CHRONICALLY IRRADIATED PERSONS AT LATE PERIOD AFTER EXPOSURE

Akleyev A.A., Nikiforov V.S., Blinova E.A., Dolgushin I.I.

Abstract

At late period after exposure a decrease in the level of NF-kB1gene expression and an increase in the level of PAD4 gene expressionwere registered in exposed persons. Considering the dependence of some immunity parameters on the levels of NF-kB1 and PAD4geneexpression, it can be assumed that changes in the immunity of exposed personsin the remote period can be mediated by changes in transcriptional activity of genes.
Russian Journal of Immunology. 2019;22(2-2):1042-1044
pages 1042-1044 views

ASSOCIATION OF GENE POLYMORPHISMS WITH SOME INDICATORS OF ADAPTIVE IMMUNITY IN CHRONICALLY EXPOSED PERSONS

Blinova E.A., Akleyev A.A., Kotikova A.I., Yanishevskaya M.A.

Abstract

Associations between polymorphic sites rs2239815 of gene MAPK8, rs1053023 of gene STAT3, rs4143094 of gene GATA3, rs28362491 of gene NF-kB1 with the absolute and relative number of CD19+ lymphocytes, CD3+CD4+ lymphocytes and with the absolute content of CD3+ and CD3+CD8+ lymphocytes were registeredin exposed persons. 

Russian Journal of Immunology. 2019;22(2-2):1045-1047
pages 1045-1047 views

MODIFICATION HEMOGLOBIN PROFILE THE BONE MARROW AND PERIPHERAL BLOODAT RATS IN CONDITIONS OF ACUTE ASEPTIC INFLAMMATION

Brilliant S.A., Yushkov B.G., Tyumentseva N.V.

Abstract

This article presents an assessment of hemoglobin profi lethebone marrow and peripheral blood at rats in conditions acute aseptic inflammation. The produced a quantitative assessment the erythroid cells with the development of inflammation.
Russian Journal of Immunology. 2019;22(2-2):1048-1050
pages 1048-1050 views

IMMUNE REACTIVITY IN TOXIC LIVER DAMAGE OF ALCOHOL ETIOLOGY

Konoplya N.A., Dolgareva S.A., Sorokin A.V., Ryzhikova G.N.

Abstract

On 62 Wistar rats with a 30-day-long, to a greater extent with a 60-day-long ethanol introduction, the development of toxic liver damage has been established by biochemical and morphological methods. In presence of a 60-day-long chronic alcohol intoxication, compared with a 30-day-long ethanol introduction, a more pronounced suppression of adaptive cellular and humoral forms formation of the immune response to sheep erythrocytes, a decrease in the phagocytic capabilities of neutrophils with an increase in their oxygen-dependent activity have been determined.
Russian Journal of Immunology. 2019;22(2-2):1051-1053
pages 1051-1053 views

MARKERS OF INFLAMMATION AND OXIDATIVE STRESS IN THE TREATMENT OF ACUTE ISCHEMIA OF THE LOWER LIMBS

Magamedov I.D., Pivovarova L.P., Ariskina O.B., Nohrin S.P., Soroka V.V.

Abstract

Acute ischemia occurs due to obstruction of blood circulation, causing oxidative stress and tissue destruction. Restoration of blood flow ‒ reperfusion syndrome ‒ tissue antigens rush into the bloodstream, increasing the production of ROS and stimulating inflammation. The purpose of the work is to determine the relationship of oxidative stress, inflammation with the severity and outcome of the disease. 44 patients with acute ischemia of the lower limbs and basic therapy were observed. Studied the change in the process of treating blood oxidative activity by the level of chemiluminescence, total antioxidant activity plasma, C-reactive protein level as a marker of inflammation and creatine kinase, reflecting tissue damage, white blood cell count with leukogram count and neutrophilic lymphocyte ratio. Acute ischemia of the lower limbs is accompanied by destruction of tissues and an increase in ROS production, a decrease in total antioxidant activity, and an increase in systemic inflammation during the reperfusion period. With an increase in the severity of the disease, an increase in oxidative stress is observed, which exacerbates the destruction of tissues and provokes a further increase in the systemic inflammatory response.
Russian Journal of Immunology. 2019;22(2-2):1054-1056
pages 1054-1056 views

THE ROLE OF CANDIDA ALBICANS IN THE DEVELOPMENT OF FOOD HYPERSENSITIVITY AND METABOLIC DISORDERS

Novikov P.S., Cherevko N.A., Denisenko O.A., Kondakov S.E.

Abstract

Currently, Candida albicans is considered a predictor of chronic infl ammation and insulin resistance. The work investigated the contribution of fungal sensitization on the example of Candida albicans in the development of hypersensitivity to food antigens and manifestations of the metabolic syndrome.
Russian Journal of Immunology. 2019;22(2-2):1057-1059
pages 1057-1059 views

THE INFLUENCE OF DIFFERENT PHYSICAL ACTIVITY ON THE STRUCTURE OF LYMPHOID ORGANS IN RATS OF DIFFERENT SEX

Khramtsova Y.S., Tyumentseva N.V., Altabaeva E.N., Artashyan O.S.

Abstract

Assuming that the peculiarities of structural and functional changes in lymphoid organs depend on the intensity and duration of physical training, the aim of the work was to study the effect of different types of physical activity on the central and peripheral organs of immunogenesis (thymus and spleen) in rats of different sexes. It is shown that regardless of sexual differences different modes of physical training cause the same type of structural changes in the lymphoid organs, which lead to a compensatory restructuring of the immune system in response to stress.
Russian Journal of Immunology. 2019;22(2-2):1060-1062
pages 1060-1062 views

STUDYING THE ANTIMICROBIAL ACTION OF TRIMETHYLAMMONIUM HEXACHLOROPLATINATE AGAINST E. COLI

Shlepotina N.M., Kolesnikov O.L., Shishkova Y.S., Galagudin I.V., Tkachyova A.R., Sharutin V.V.

Abstract

This study represents one of the stages of the study of the antimicrobial action of trimethylammonium hexachloroplatinate on bacteria Escherichia coli.
Russian Journal of Immunology. 2019;22(2-2):1063-1065
pages 1063-1065 views


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